Dry biorefining maximizes the potentials of simultaneous saccharification and co‐fermentation for cellulosic ethanol production

Abstract

Despite the well-recognized merits of simultaneous saccharification and cofermentation (SSCF) on relieving sugar product inhibition on cellulase activity, a practical concomitance difficulty of xylose with inhibitors in the pretreated lignocellulose feedstock prohibits the essential application of SSCF for cellulosic ethanol fermentation. To maximize the SSCF potentials for cellulosic ethanol production, a dry biorefining approach was proposed starting from dry acid 60 |

Year-Round Storage Operation of Three Major Agricultural Crop Residue Biomasses by Performing Dry Acid Pretreatment at Regional Collection Depots

“…Compared with our previous studies, the ethanol production in this study was relatively low (64.7 versus 78.7 g/L for corn stover, 63.8 versus 88.0 g/L for wheat straw, or by ethanol yield of 75.7 versus 84.7% for corn stover, 73.9 versus 82.8% for wheat straw). The major reason came from the higher ash content (7.4–10.5%) because no deashing step was conducted in this study for the purpose of achieving the realistic scenario of storage.…”

“…The overall ethanol yield from the dry corn stover, wheat straw, or rice straw was 75.7, 83.8, and 76.4% of the theoretical values, respectively. The results are based on the experimental results of year-round storage using the crop residue feedstocks without the de-ashing operation, therefore, the ethanol yield and titer were lower than those while using the de-ashed feedstocks, which is a conventional operation. , …”

“…The results are based on the experimental results of year-round storage using the crop residue feedstocks without the de-ashing operation, therefore, the ethanol yield and titer were lower than those while using the de-ashed feedstocks, which is a conventional operation. 28,30 The feedstock transportation cost accounts for 35−50% of the overall production cost of cellulosic ethanol. 49 The present year-round storage result supports a feasible low-cost transportation strategy because of the high and constant feedstock density after dry-acid pretreatment.…”

Year-Round Storage Operation of Three Major Agricultural Crop Residue Biomasses by Performing Dry Acid Pretreatment at Regional Collection Depots

“…Compared with our previous studies, the ethanol production in this study was relatively low (64.7 versus 78.7 g/L for corn stover, 63.8 versus 88.0 g/L for wheat straw, or by ethanol yield of 75.7 versus 84.7% for corn stover, 73.9 versus 82.8% for wheat straw). The major reason came from the higher ash content (7.4–10.5%) because no deashing step was conducted in this study for the purpose of achieving the realistic scenario of storage.…”

“…The overall ethanol yield from the dry corn stover, wheat straw, or rice straw was 75.7, 83.8, and 76.4% of the theoretical values, respectively. The results are based on the experimental results of year-round storage using the crop residue feedstocks without the de-ashing operation, therefore, the ethanol yield and titer were lower than those while using the de-ashed feedstocks, which is a conventional operation. , …”

“…The results are based on the experimental results of year-round storage using the crop residue feedstocks without the de-ashing operation, therefore, the ethanol yield and titer were lower than those while using the de-ashed feedstocks, which is a conventional operation. 28,30 The feedstock transportation cost accounts for 35−50% of the overall production cost of cellulosic ethanol. 49 The present year-round storage result supports a feasible low-cost transportation strategy because of the high and constant feedstock density after dry-acid pretreatment.…”

In-Depth Two-Stage Transcriptional Reprogramming and Evolutionary Engineering of Saccharomyces cerevisiae for Efficient Bioethanol Production from Xylose with Acetate

“…These results demonstrate that improved WXY74E produced 58.4 g/L of ethanol with 65.1% of the theoretical ethanol yield, which is higher than that previously reported for haploid XR-XDH strains by SSCF (Table S1). Approximately half of the ethanol was produced from the initial glucose released during prehydrolysis, and the other half was produced from xylose and glucose released during SSCF . This adapted S.…”

Lysine Production by Dry Biorefining of Wheat Straw and Cofermentation of Corynebacterium glutamicum

“…Solid-state biodetoxification was conducted on the pretreated wheat straw solids by A. resinae ZN1 until furfural and HMF were completely degraded (not detected by HPLC). − Xylose was still well retained in the biodetoxified wheat straw at 0.113 ± 0.005 g/g DM. The wheat straw hydrolysate was prepared by enzymatic hydrolysis to obtain 33.3 ± 1.7 g/L xylose and 100.2 ± 0.6 g/L glucose.…”

Year-Round Storage Operation of Three Major Agricultural Crop Residue Biomasses by Performing Dry Acid Pretreatment at Regional Collection Depots

“…Compared with our previous studies, the ethanol production in this study was relatively low (64.7 versus 78.7 g/L for corn stover, 63.8 versus 88.0 g/L for wheat straw, or by ethanol yield of 75.7 versus 84.7% for corn stover, 73.9 versus 82.8% for wheat straw). The major reason came from the higher ash content (7.4–10.5%) because no deashing step was conducted in this study for the purpose of achieving the realistic scenario of storage.…”

“…The overall ethanol yield from the dry corn stover, wheat straw, or rice straw was 75.7, 83.8, and 76.4% of the theoretical values, respectively. The results are based on the experimental results of year-round storage using the crop residue feedstocks without the de-ashing operation, therefore, the ethanol yield and titer were lower than those while using the de-ashed feedstocks, which is a conventional operation. , …”

“…The results are based on the experimental results of year-round storage using the crop residue feedstocks without the de-ashing operation, therefore, the ethanol yield and titer were lower than those while using the de-ashed feedstocks, which is a conventional operation. 28,30 The feedstock transportation cost accounts for 35−50% of the overall production cost of cellulosic ethanol. 49 The present year-round storage result supports a feasible low-cost transportation strategy because of the high and constant feedstock density after dry-acid pretreatment.…”

In-Depth Two-Stage Transcriptional Reprogramming and Evolutionary Engineering of Saccharomyces cerevisiae for Efficient Bioethanol Production from Xylose with Acetate

“…These results demonstrate that improved WXY74E produced 58.4 g/L of ethanol with 65.1% of the theoretical ethanol yield, which is higher than that previously reported for haploid XR-XDH strains by SSCF (Table S1). Approximately half of the ethanol was produced from the initial glucose released during prehydrolysis, and the other half was produced from xylose and glucose released during SSCF . This adapted S.…”

Lysine Production by Dry Biorefining of Wheat Straw and Cofermentation of Corynebacterium glutamicum

“…Solid-state biodetoxification was conducted on the pretreated wheat straw solids by A. resinae ZN1 until furfural and HMF were completely degraded (not detected by HPLC). − Xylose was still well retained in the biodetoxified wheat straw at 0.113 ± 0.005 g/g DM. The wheat straw hydrolysate was prepared by enzymatic hydrolysis to obtain 33.3 ± 1.7 g/L xylose and 100.2 ± 0.6 g/L glucose.…”

Year-Round Storage Operation of Three Major Agricultural Crop Residue Biomasses by Performing Dry Acid Pretreatment at Regional Collection Depots

“…Compared with our previous studies, the ethanol production in this study was relatively low (64.7 versus 78.7 g/L for corn stover, 63.8 versus 88.0 g/L for wheat straw, or by ethanol yield of 75.7 versus 84.7% for corn stover, 73.9 versus 82.8% for wheat straw). The major reason came from the higher ash content (7.4–10.5%) because no deashing step was conducted in this study for the purpose of achieving the realistic scenario of storage.…”

“…The overall ethanol yield from the dry corn stover, wheat straw, or rice straw was 75.7, 83.8, and 76.4% of the theoretical values, respectively. The results are based on the experimental results of year-round storage using the crop residue feedstocks without the de-ashing operation, therefore, the ethanol yield and titer were lower than those while using the de-ashed feedstocks, which is a conventional operation. , …”

“…The results are based on the experimental results of year-round storage using the crop residue feedstocks without the de-ashing operation, therefore, the ethanol yield and titer were lower than those while using the de-ashed feedstocks, which is a conventional operation. 28,30 The feedstock transportation cost accounts for 35−50% of the overall production cost of cellulosic ethanol. 49 The present year-round storage result supports a feasible low-cost transportation strategy because of the high and constant feedstock density after dry-acid pretreatment.…”

In-Depth Two-Stage Transcriptional Reprogramming and Evolutionary Engineering of Saccharomyces cerevisiae for Efficient Bioethanol Production from Xylose with Acetate

“…These results demonstrate that improved WXY74E produced 58.4 g/L of ethanol with 65.1% of the theoretical ethanol yield, which is higher than that previously reported for haploid XR-XDH strains by SSCF (Table S1). Approximately half of the ethanol was produced from the initial glucose released during prehydrolysis, and the other half was produced from xylose and glucose released during SSCF . This adapted S.…”

Lysine Production by Dry Biorefining of Wheat Straw and Cofermentation of Corynebacterium glutamicum

“…Solid-state biodetoxification was conducted on the pretreated wheat straw solids by A. resinae ZN1 until furfural and HMF were completely degraded (not detected by HPLC). − Xylose was still well retained in the biodetoxified wheat straw at 0.113 ± 0.005 g/g DM. The wheat straw hydrolysate was prepared by enzymatic hydrolysis to obtain 33.3 ± 1.7 g/L xylose and 100.2 ± 0.6 g/L glucose.…”