2021
DOI: 10.1021/acs.jafc.0c07588 View full text |Buy / Rent full text
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Abstract: Engineering an artificial microbial community for natural product production is a promising strategy. As mono-and dual-culture systems only gave non-detectable or minimal chlorogenic acid (CGA) biosynthesis, here, a polyculture of three recombinant Escherichia coli strains, acting as biosynthetic modules of caffeic acid (CA), quinic acid (QA), and CGA, was designed and used for de novo CGA biosynthesis. An influx transporter of 3-dehydroshikimic acid (DHS)/shikimic acid (SA), ShiA, was introduced into the QA m… Show more

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