A comparative proteomic analysis of blood serum for developmental stages in pigs

Abstract

This study aimed to differentiate genes at developmental stages of pigs from 0 to 150 days of age, to build up a protein database and to find candidate genetic markers for growth traits. The analysis of two-dimensional electrophoresis and matrix-assisted laser-desorption/ionization mass spectrometry separated 252 protein segments. After successfully blasting the peptide sequences, the analysis confirmed 37 differentially expressed proteins that increased from birth to 150 days of age (type A), whereas the type B proteins presented the inverse pattern. The type C proteins included proteins that were expressed continuously throughout the developmental periods. A total of 319 primer sets for 33 genes were designed to find genetic variants using pooled DNA samples of Yorkshire pigs. Amplification products for all primer sets produced approximately 20 000 clones that were sequenced, and 48 candidate SNP sites were finalized for genotyping. A total of 475 animals were used for high throughput genotyping analysis. Among these, phenotype data of all 475 animals were collected for average daily gain, backfat thickness and days to 90 kg, whereas feed conversion data were collected for 300 animals and body measurement traits (starting weight, ending weight, body length, wither height and chest depth) were collected for 209 animals. Association analysis found significant statistical differences between the animals having genotypes of 13 SNPs (g.78935883C>T, g.147629986C>T, g.98266037T>C, g.214707340G>A, g.88350299C>T, g.17180956C>T, g.17181024C>T, g.2350283A>G, g.138361311C>T, g.44996379C>T, g.44996247A>C, g.107715245C>T, g.4149631C>T) for the various measured traits. The identified genetic polymorphisms, of which one was novel (g.214707340G>A), may serve as candidate molecular markers to change population means for the targeted growth traits.

Comparative Proteomic Analysis of Serum from Pigs Experimentally Infected with Trichinella spiralis, Trichinella britovi, and Trichinella pseudospiralis

“…According to literature data, swine serum is one of the most intensively studied matrices by various proteomic techniques. Two-dimensional gel electrophoresis (2-DE) has been used to create reference serum proteomic maps for both healthy adult pigs [17] and growing piglets [18,19]. Furthermore, various proteomic tools such as surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI TOF-MS); two-dimensional and two-dimensional differential gel electrophoresis (2-DE/2D-DIGE) followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS); or liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) have also been applied to evaluate serum proteomic profiles of (1) pigs with acute liver failure [20]; (2) pigs exposed to stress factors, such as insufficient space [21]; (3) pigs suffering from low-dose LPS-induced inflammation [22]; and (4) pigs experimentally or naturally infected with classical swine fever virus [23,24], food-and-mouth disease virus [25] or porcine reproductive and respiratory syndrome virus [26].…”