Activation of the Receptor NKG2D Leads to Production of Th17 Cytokines in CD4+ T Cells of Patients With Crohn's Disease

Phenotypic analysis of T cells infiltrating colon cancers: Correlations with oncogenetic status

“…3A, B). These results are consistent with our previous observations in another model 13,14 and indicate that NKG2D-positive CD4 T cells might have cytotoxic functions.…”

“…NKG2D-expressing CD4 T cells harboring increased effector functions are implicated in inflammatory bowel diseases 13,14 and may also play a role in antitumor immunity. 15,16 We analyzed the phenotype and function of these cells in the mucosa and tumor of colon cancer patients (Fig.…”

“…13,14 After isolation from the tumor, mucosal tissue, or blood, T cells were activated overnight with CD3-coated target cells. CD4 and CD8 T cells from PBL were activated, as demonstrated by upregulation of the activation markers CD69 and CD25 at their surface (Fig.…”

DNA demethylation and histone H3K9 acetylation determine the active transcription of the NKG2D gene in human CD8+T and NK cells

“…alba Fernández-sánchez, 1 aroa Baragaño Raneros, 1 Reyes carvajal palao, 1 ana B. sanz, 2 alberto Ortiz, 3,4 Francisco Ortega, 4,5 Beatriz suárez-Álvarez 1, † and carlos López-Larrea…”

Application of user-guided automated cytometric data analysis to large-scale immunoprofiling of invariant natural killer T cells

“…Functional characterization of iNKT cells requires comprehensive assessment of surface expression of homing receptors, lectins, and cell adhesion molecules, as well as cytokine production. Immunoprofiling studies have yet to assay such a comprehensive set of markers in primary iNKT cells in a sufficiently large cohort (18,(20)(21)(22)(23)(24). Here, we profiled iNKT cells in 110 subjects with 19 surface and intracellular markers.…”