Association between rumen microbiota and marbling grade in Hu sheep

Wang, Jianghui; Zhang, Yukun; Wang, Xiaojuan; Li, Fadi; Zhang, Deyin; Li, Xiaolong; Zhao, Yuan; Zhao, Liming; Xu, Dan; Cheng, Jiangbo; Li, Wenxin; Lin, Changchun; Yang, Xiaobin; Zhai, Rui; Zeng, Xiwen; Cui, Panpan; Ma, Zongwu; Liu, Jia; Zhang, Xiaoxue; Wang, Weimin

doi:10.3389/fmicb.2022.978263

Cited by 4 publications

(3 citation statements)

References 42 publications

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“…Firmicutes are associated with fat deposition in mice 72 . Similarly, in ruminants, the relative abundance of Firmicutes was higher in high-marbling sheep than in low- or middle-marbling sheep 9 . Thus, Firmicutes_A MAGs in the rumen may play a pivotal role in increasing the IMF content in beef cattle.…”

Section: Resultsmentioning

confidence: 85%

“…Rumen-related functions, such as microbial degradation and protein synthesis, are associated with these traits, as well as other factors, including genetics 7 , feeds 7 , and blood metabolites 8 . For example, VFAs produced by rumen microorganisms are associated with intramuscular fat (IMF) content in sheep 9 . Additionally, Japanese black (JB) cattle produce high marbling compared to other breeds 10 , 11 , with taxonomical and functional differences in the rumen microbiome of crossbreeds 12 .…”

Section: Introductionmentioning

confidence: 99%

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Impact of rumen microbiome on cattle carcass traits

Sato,

Fukui

et al. 2024

Sci Rep

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Rumen microbes are crucial in the anaerobic fermentation of plant polysaccharides to produce volatile fatty acids. However, limited information exists about the specific microbial species and strains in the rumen that affect carcass traits, and it is unclear whether there is a relationship between rumen metabolic functions and these traits. This study investigated the relationship between the rumen microbiome and carcass traits in beef cattle using 16S rRNA amplicon and shotgun sequencing. Metagenomic sequencing was used to compare the rumen microbiome between high-carcass weight (HW) and low-carcass weight (LW) cattle, and high-marbling (HM) and low-marbling (LM) cattle. Prokaryotic communities in the rumen of HW vs. LW and HM vs. LM were separated using 16S rRNA amplicon sequencing. Notably, shotgun metagenomic sequencing revealed that HW cattle had more methane-producing bacteria and ciliate protozoa, suggesting higher methane emissions. Additionally, variations were observed in the abundances of certain glycoside hydrolases and polysaccharide lyases involved in the ruminal degradation of plant polysaccharides between HW and LW. From our metagenome dataset, 807 non-redundant metagenome-assembled genomes (MAGs) of medium to high quality were obtained. Among these, 309 and 113 MAGs were associated with carcass weight and marbling, respectively.

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Section: Resultsmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Impact of rumen microbiome on cattle carcass traits

Sato,

Fukui

et al. 2024

Sci Rep

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“…As with propionate, acetate and butyrate both serve as energy sources for the host, and butyrate in particular is an important energy source for rumen epithelial cells (Remond et al 1995 ). Acetate and butyrate are also lipogenic, and rumen concentrations of both SCFAs have been positively correlated with fat content of lamb (Wang et al 2022 ). Christensenellaceae R-7 has also been reported to be enriched in the rumen microbiota of beef cattle fed high-fiber diets (Li et al 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Associations between the rumen microbiota and carcass merit and meat quality in beef cattle

Holman,

Gzyl,

Scott

et al. 2024

Appl Microbiol Biotechnol

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The rumen microbiota is important for energy and nutrient acquisition in cattle, and therefore its composition may also affect carcass merit and meat quality attributes. In this study, we examined the associations between archaeal and bacterial taxa in the rumen microbiota of beef cattle and 12 different attributes, including hot carcass weight (HCW), dressing percentage, ribeye area (REA), intramuscular fat content, marbling score, fat thickness, yield grade, moisture content, purge loss, and shear force. There were significant correlations between the relative abundance of certain archaeal and bacterial genera and these attributes. Notably, Selenomonas spp. were positively correlated with live weight and HCW, while also being negatively correlated with purge loss. Members of the Christensenellaceae R-7, Moryella, and Prevotella genera exhibited positive and significant correlations with various attributes, such as dressing percentage and intramuscular fat content. Ruminococcaceae UCG-001 was negatively correlated with live weight, HCW, and dressing percentage, while Acidaminococcus and Succinivibrionaceae UCG-001 were negatively correlated with intramuscular fat content, moisture content, and marbling score. Overall, our findings suggest that specific changes in the rumen microbiota could be a valuable tool to improve beef carcass merit and meat quality attributes. Additional research is required to better understand the relationship between the rumen microbiota and these attributes, with the potential to develop microbiome-targeted strategies for enhancing beef production. Key points • Certain rumen bacteria were associated with carcass merit and meat quality • Moryella was positively correlated with intramuscular fat in beef carcasses • Acidaminococcus spp. was negatively correlated with marbling and intramuscular fat

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Relationship between Rumen Microbial Differences and Phenotype Traits among Hu Sheep and Crossbred Offspring Sheep

Wang,

Zhan,

Jia

et al. 2024

Animals

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This experiment was conducted to investigate the effect of three−way hybrid sheep and Hu sheep on serum indicators, rumen fermentation, rumen enzyme activity, and microorganisms in sheep. Healthy and similar birth weights from three groups (Hu, n = 11; Charolais × Australian White × Hu, CAH, n = 11; Charolais × Dorper × Hu, CDH, n = 11) were selected to be fed by the ewes until 45 days of age. Subsequently, they were weaned intensively and underwent short−term fattening for 3 months along with selected male lambs fed intensively. During this period, they were fed and watered ad libitum. Blood and rumen fluid were collected and analyzed for serum indicators and rumen fluid microorganisms, enzyme activity, and VFA, respectively, at the end of the fattening period. Compared with Hu lamb, the offspring of the three−way hybrid lamb showed significant improvements in body weight, serum lactate dehydrogenase, and creatinine content. However, there was no significant effect on serum immunity and antioxidant indices. In addition, the rumen fluid volatile fatty acid (VFA) molar concentration and microcrystalline cellulose and lipase content were significantly lower in the three−way hybrid lamb compared to Hu lamb, but β−glucosidase, amylase, pepsin, and VFA molar ratio were not significantly affected. Subsequently, 16S rRNA sequencing diversity analysis revealed that three−way hybrid lamb significantly increased rumen microbial ACE and Chao1 indices compared to Hu lamb. Meanwhile, the abundance of Verrucomicrobiota and Synergistota significantly increased at the phylum level. Correlation analysis showed that Prevotella had the highest proportion, while Rikenellaceae_RC9_gut_group correlated most closely with others genus. The microbial communities isovaleric acid molar concentration and proportion were strongly correlated. In addition, there were significant differences in correlations between microbial communities and isobutyric acid, butyric acid and valeric acid content, and their molar proportion, but they were not significantly correlated with digestive enzymes. From the functional enrichment analysis, it was found that hybrid progeny were mainly enriched in the pyruvate metabolism, microbial metabolism in diverse environments, carbon metabolism, and quorum sensing pathways. In contrast, the Hu sheep were primarily enriched in the cysteine and methionine, amino sugar and nucleotide sugar, and biosynthesis of secondary metabolite pathways. These results suggest that hybridization can play a role in regulating organismal metabolism and improve animal production performance by influencing the structure and characteristics of microbial communities.

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Association between rumen microbiota and marbling grade in Hu sheep

Cited by 4 publications

References 42 publications

Impact of rumen microbiome on cattle carcass traits

Impact of rumen microbiome on cattle carcass traits

Associations between the rumen microbiota and carcass merit and meat quality in beef cattle

Relationship between Rumen Microbial Differences and Phenotype Traits among Hu Sheep and Crossbred Offspring Sheep

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