Clinical Ketosis-Associated Alteration of Gene Expression in Holstein Cows

Wu, Zhoulin; Chen, Shiyi; Qin, Chao; Jia, Xianbo; Deng, Feilong; Wang, Jie; Lai, Songjia

doi:10.3390/genes11020219

Cited by 12 publications

(7 citation statements)

References 55 publications

(62 reference statements)

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“…These pathways identified at a metabolomic level will ultimately improve our understanding of ketosis. At a proteomic level, KEGG pathway analysis indicated that these proteins were involved in disease-related pathways, such as amebiasis, vitamin digestion and absorption, phagosome, legionellosis, and ECM-receptor interaction; part of these results are in accordance with our previous work based on transcriptomic analysis (Wu et al, 2020) and work from others (Xu and Wang, 2008;Trevisi and Minuti, 2018). It was difficult to uncover molecular mechanisms for ketosis during the transition period although the level of each compound and the abundance of each protein could be determined.…”

Section: Discussionsupporting

confidence: 90%

“…All experimental procedures involved in this study were approved by the Institutional Animal Care and Use Committee of Sichuan Agricultural University (DKY-B20171906). The current study is a continuation of previous research, where the differentially expressed genes and pathways associated with ketosis were investigated using these animals (Wu et al, 2020). The animals and experimental design were fully described in the original article.…”

Section: Experimental Animals and Blood Serum Samplesmentioning

confidence: 99%

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Metabolomic and Proteomic Profiles Associated With Ketosis in Dairy Cows

Chen

et al. 2020

Front. Genet.

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Ketosis is a common metabolic disease in dairy cows during early lactation. However, information about the metabolomic and proteomic profiles associated with the incidence and progression of ketosis is still limited. In this study, an integrated metabolomics and proteomics approach was performed on blood serum sampled from cows diagnosed with clinical ketosis (case, ≥ 2.60 mmol/L plasma β-hydroxybutyrate; BHBA) and healthy controls (control, < 1.0 mmol/L BHBA). Samples were taken 2 weeks before parturition and 2 weeks after parturition from 19 animals (nine cases, 10 controls). All serum samples (n = 38) were subjected to Liquid Chromatography-Mass Spectrometry (LC-MS) based metabolomic analysis, and 20 samples underwent Data-Independent Acquisition (DIA) LC-MS based proteomic analysis. A total of 97 metabolites and 540 proteins were successfully identified, and multivariate analysis revealed significant differences in both metabolomic and proteomic profiles between cases and controls. We investigated clinical ketosis-associated metabolomic and proteomic changes using statistical analyses. Correlation analysis of statistically significant metabolites and proteins showed 78 strong correlations (correlation coefficient, R ≥ 0.7) between 38 metabolites and 25 proteins, which were then mapped to pathways using IMPaLA. Results showed that ketosis altered a wide range of metabolic pathways, such as metabolism, metabolism of proteins, gene expression and post-translational protein modification, vitamin metabolism, signaling, and disease related pathways. Findings presented here are relevant for identifying molecular targets for ketosis and biomarkers for ketosis detection during the transition period.

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Section: Discussionsupporting

confidence: 90%

Section: Experimental Animals and Blood Serum Samplesmentioning

confidence: 99%

Metabolomic and Proteomic Profiles Associated With Ketosis in Dairy Cows

Chen

et al. 2020

Front. Genet.

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“…However, there were 95 DEGs among postpartum and prepartum sick cows, which revealed depressed variations of the gene expression in the transition interval compared to healthy cows (428 DEGs). Functional examination shows that DEGs linked with ketosis were the fundamental reason for biological stress response, ion homeostasis, amino acids metabolism, energy signaling, and disease-related processes [ 86 ].…”

Section: Strategies To Alleviate Biological Stress During the Transit...mentioning

confidence: 99%

Nutrigenomic Interventions to Address Metabolic Stress and Related Disorders in Transition Cows

Hassan

Nadeem

Javed

et al. 2022

BioMed Research International

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For dairy cattle, the period involving a shift from late pregnancy to early lactation termed transition or periparturient is an excruciating phase. Health-related disorders are likely to happen in this time frame. Timely postpartum and metabolic adjustments to this new physical state demands correct management strategies to fulfill the cow’s needs for a successful transition to this phase. Among the management strategies, one of the most researched methods for managing transition-related stress is nutritional supplementation. Dietary components directly or indirectly affect the expression of various genes that are believed to be involved in various stress-related responses during this phase. Nutrigenomics, an interdisciplinary approach that combines nutritional science with omics technologies, opens new avenues for studying the genome’s complicated interactions with food. This revolutionary technique emphasizes the importance of food-gene interactions on various physiological and metabolic mechanisms. In animal sciences, nutrigenomics aims to promote the welfare of livestock animals and enhance their commercially important qualities through nutritional interventions. To this end, an increasing volume of research shows that nutritional supplementation can be effectively used to manage the metabolic stress dairy cows undergo during the transition period. These nutritional supplements, including polyunsaturated fatty acids, vitamins, dietary amino acids, and phytochemicals, have been shown to modulate energy homeostasis through different pathways, leading to addressing metabolic issues in transition cows.

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“…A volcano plot visualizing the expression of DEGs was created using the “ggplots 2” package (v. 3.3.2). A heatmap was further produced using the centered and scaled FPKM values of DEGs in the “Pheatmap” package (v.1.0.12) ( Wu et al, 2020 ). The DEGs in the SMX-treated group was submitted for the functional enrichment analyses of gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) pathway.…”

Section: Methodsmentioning

confidence: 99%

Sulfamethoxazole-Altered Transcriptomein Green Alga Raphidocelis subcapitata Suggests Inhibition of Translation and DNA Damage Repair

Guo

Zhang

et al. 2021

Front. Microbiol.

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Occurrence of sulfonamide antibiotics has been reported in surface waters with the exposures ranging from < 1 ng L–1 to approximately 11 μg L–1, which may exert adverse effects on non-target algal species, inhibiting algal growth and further hindering the delivery of several ecosystem services. Yet the molecular mechanisms of sulfonamide in algae remain undetermined. The aims of the present work are: (1) to test the hypothesis whether sulfamethoxazole (SMX) inhibits the folate biosynthesis in a model green alga Raphidocelis subcapitata; and (2) to explore the effects of SMX at an environmentally relevant concentration on algal health. Here, transcriptomic analysis was applied to investigate the changes at the molecular levels in R. subcapitata treated with SMX at the concentrations of 5 and 300 μg L–1. After 7-day exposure, the algal density in the 5 μg L–1 group was not different from that in the controls, whereas a marked reduction of 63% in the high SMX group was identified. Using the adj p < 0.05 and absolute log2 fold change > 1 as a cutoff, we identified 1 (0 up- and 1 downregulated) and 1,103 (696 up- and 407 downregulated) differentially expressed genes (DEGs) in the 5 and 300 μg L–1 treatment groups, respectively. This result suggested that SMX at an environmentally relevant exposure may not damage algal health. In the 300 μg L–1 group, DEGs were primarily enriched in the DNA replication and repair, photosynthesis, and translation pathways. Particularly, the downregulation of base and nucleotide excision repair pathways suggested that SMX may be genotoxic and cause DNA damage in alga. However, the folate biosynthesis pathway was not enriched, suggesting that SMX does not necessarily inhibit the algal growth via its mode of action in bacteria. Taken together, this study revealed the molecular mechanism of action of SMX in algal growth inhibition.

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Clinical Ketosis-Associated Alteration of Gene Expression in Holstein Cows

Cited by 12 publications

References 55 publications

Metabolomic and Proteomic Profiles Associated With Ketosis in Dairy Cows

Metabolomic and Proteomic Profiles Associated With Ketosis in Dairy Cows

Nutrigenomic Interventions to Address Metabolic Stress and Related Disorders in Transition Cows

Sulfamethoxazole-Altered Transcriptomein Green Alga Raphidocelis subcapitata Suggests Inhibition of Translation and DNA Damage Repair

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