Comparison of two histopathologic methods for evaluating subcutaneous reaction to mineral trioxide aggregate

Abstract

Objectives: One of the most important factors for suitable materials for pulp therapy is biocompatibility. Two histopathologic methods of Cox and Federation Dentaire International (FDI) were used to evaluate inflammation. In Cox method, density of inflammatory cells, tissue reactions like fibrosis, vascular responses like congestion and fibrin extravasation have been used to evaluate inflammatory reactions. The aim of this study was to compare the accuracy of pathologists’ interpretations using two different methods. Study design: Three pathologists observed the degree of inflammation in 225 histopathologic sections. These sections showed inflammation in subcutaneous connective tissue of rats adjacent to polyethylene tubes, filled with white or gray mineral trioxide aggregate. Empty tubes served as controls. Samples were harvested after 7-, 15-, 30-, 60-, and 90-days. All pathologists examined the sections under a light microscope (Carl Zeiss, Oberkochen, Germany) at ×400 magnifications. Chi-Square test was used to evaluate the difference between inflammation grades when one pathologist used two methods. Cohen’s Kappa value was used to measure agreement of three pathologists to recognize the degrees of inflammations when using one of the methods. Results: There were no significant differences between the two methods when one of the pathologist used these methods to report the degree of inflammation (p=0.054). However, two other pathologists reported significant differences between two methods (p=0.005, p=0.001). In the FDI method, there was an acceptable agreement between first and second, and first and third pathologist in terms of the degree of inflammation, and intermediate agreement existed between the second and third pathologist. With the Cox method, no agreement among the pathologists could be found. Conclusion: The results of three pathologists in terms of rating inflammation with the FDI method showed better agreement than with the Cox method. Therefore, FDI method is more reliable than the Cox method to evaluate inflammation. Key words: Biocompatibility, connective tissue, infammation, mineral trioxide aggregate.

Morphological and immunohistochemical analysis of the biocompatibility of resin‐modified cements

“…The monomers are capable of triggering significant cytotoxicity and interfere directly in the biocompatibility of these composites (Ahrari, Tavakkol Afshari, Poosti, & Brook, ; Mathisen et al, ). The cements RS and RMO contain BisGMA, a monomer that presents low degree of conversion values, and they consequently tend to remain residual, even after complete polymerization (Costa et al, ; Lacerda‐Santos et al, ; Vosoughhosseini et al, ). Whereas the cement TP contains the monomer 2‐hydroxy‐1,3‐dimethacryloxypropane which, in spite of having a good degree of conversion, may also trigger cytotoxicity to oral tissues (Malkoc, Corekci, Botsali, Yalçin, & Sengun, ).…”

“…In the analysis quantifying the macrophages present in the tissue reactions, a higher level of intensity of these cells could be observed in the time intervals of 7 and 15 days. This initial inflammatory response reflects the tissue attempts to induce the process of degradation of the materials by means of the MOs recruited to the surrounding tissue and in the direction toward the surface of the cements (Vosoughhosseini et al, ). The activated MOs play an important role in the phagocytosis of these foreign materials, recruitment of fibroblasts, and in the release of inflammatory mediators (Kochanowski et al, ).…”

Effect of Yellow Propolis on Biocompatibility of Cements: Morphological and Immunohistochemistry Analysis

“…MOs are responsible for potentiating the proinflammatory response 41 ; they act in the presentation of antigens, phagocytosis, recruitment of fibroblasts, 42 degradation and/or isolation of the cement 10 and substances of a toxic nature. 43 The effects of the factor cement, its components, and alcohol on tissue response, appear to have been more significant in the cements with 10% EEP, because at the higher concentrations of EEP the inflammatory and healing conditions were similar to those of the Controls.…”

Evaluation in vivo of biocompatibility of differents resin-modified cements for bonding orthodontic bands

“…Although this methodology does not faithfully reproduce the conditions in the oral cavity, it provides important preliminary information about the biologic properties of dental materials and characteristics of tissue reactions (Aminozarbian et al 2012, Boaventura et al 2012, Yang et al 2012, Viola et al 2012). The histological parameters were evaluated after time intervals of 7, 15 and 30 days, as observed in other studies (Cunha et al 2011, Lacerda-Santos et al 2014, Santos et al 2014b, Vosoughhosseini et al 2012.…”