Diphyllin Improves High-Fat Diet-Induced Obesity in Mice Through Brown and Beige Adipocytes

Duan, Ya Nan; Ge, Xiang; Jiang, Hao; Zhang, Hongjie; Zhao, Yu; Li, Jin Long; Zhang, Wei; Li, Jing Ya

doi:10.3389/fendo.2020.592818

Cited by 11 publications

(3 citation statements)

References 64 publications

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“…Here, we found that the expression of MFAP5 was significantly down-regulated during adipocyte differentiation and maturation. We verified the sequencing results and demonstrated the function of MFAP5 in adipogenesis by silencing or overexpression in C3H10 and 3T3-L1 cells [29][30][31][32] . As mesenchymal stem cells, C3H10 cells are more primitive than 3T3-L1 cells and have multidirectional differentiation ability.…”

Section: Discussionsupporting

confidence: 62%

Microfibrillar-associated protein 5 suppresses adipogenesis by inhibiting essential coactivator of PPARγ

Zhang

Sun

et al. 2023

Sci Rep

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Femoral head necrosis is responsible for severe pain and its incidence is increasing. Abnormal adipogenic differentiation and fat cell hypertrophy of bone marrow mesenchymal stem cells increase intramedullary cavity pressure, leading to osteonecrosis. By analyzing gene expression before and after adipogenic differentiation, we found that Microfibril-Associated Protein 5 (MFAP5) is significantly down-regulated in adipogenesis whilst the mechanism of MFAP5 in regulating the differentiation of bone marrow mesenchymal stem cells is unknown. The purpose of this study was to clarify the role of MAFP5 in adipogenesis and therefore provide a theoretical basis for future therapeutic options of osteonecrosis. By knockdown or overexpression of MFAP5 in C3H10 and 3T3-L1 cells, we found that MFAP5 was significantly down-regulated as a key regulator of adipogenic differentiation, and identified the underlying downstream molecular mechanism. MFAP5 directly bound to and inhibited the expression of Staphylococcal Nuclease And Tudor Domain Containing 1, an essential coactivator of PPARγ, exerting an important regulatory role in adipogenesis.

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Section: Discussionsupporting

confidence: 62%

Microfibrillar-associated protein 5 suppresses adipogenesis by inhibiting essential coactivator of PPARγ

Zhang

Sun

et al. 2023

Sci Rep

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“…The ratio of oleic acid to palmitic acid in the diet can determine the concentration of postprandial thrombosis and fibrinolytic factors in men 47 . There is no direct evidence that cleistanthin B and diphyllin are related to thrombosis, but they have progressive effects in inducing cell apoptosis and improving obesity 48 , 49 . Unfortunately, quercetin, apigenin, luteolin, cynaroside, scopoletin, palmitic acid, cleistanthin B, and diphyllin are not unique compounds in Justicia, but it is undeniable that they contribute to the anti-thrombotic effect of Justicia.…”

Section: Discussionmentioning

confidence: 99%

Reveals of candidate active ingredients in Justicia and its anti-thrombotic action of mechanism based on network pharmacology approach and experimental validation

Hong

Zhang

et al. 2021

Sci Rep

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Thrombotic diseases seriously threaten human life. Justicia, as a common Chinese medicine, is usually used for anti-inflammatory treatment, and further studies have found that it has an inhibitory effect on platelet aggregation. Therefore, it can be inferred that Justicia can be used as a therapeutic drug for thrombosis. This work aims to reveal the pharmacological mechanism of the anti-thrombotic effect of Justicia through network pharmacology combined with wet experimental verification. During the analysis, 461 compound targets were predicted from various databases and 881 thrombus-related targets were collected. Then, herb-compound-target network and protein–protein interaction network of disease and prediction targets were constructed and cluster analysis was applied to further explore the connection between the targets. In addition, Gene Ontology (GO) and pathway (KEGG) enrichment were used to further determine the association between target proteins and diseases. Finally, the expression of hub target proteins of the core component and the anti-thrombotic effect of Justicia’s core compounds were verified by experiments. In conclusion, the core bioactive components, especially justicidin D, can reduce thrombosis by regulating F2, MMP9, CXCL12, MET, RAC1, PDE5A, and ABCB1. The combination of network pharmacology and the experimental research strategies proposed in this paper provides a comprehensive method for systematically exploring the therapeutic mechanism of multi-component medicine.

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“…Duan et al found that diphyllin intervention improved the glucolipid metabolism and insulin sensitivity, as evidenced by the increased thermogenesis in BAT and beige adipose tissues, as well as the enhanced BAT generation and WAT browning. It was speculated that diphyllin acted through inhibiting V-ATPase and intracellular autophagy (Duan et al, 2020). Besides, it is worth mentioning that aryl naphthalene lignans are widely found in various dietary or medicinal plants and have multiple biological activities.…”

Section: Promoting Bat Activity and Wat Browningmentioning

confidence: 99%

New insights of active constituents from herbs and nutraceuticals and their mechanisms in obesity-induced insulin resistance

Lin

et al. 2023

Preprint

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Insulin resistance (IR) is a main etiology of various metabolic diseases such as type 2 diabetes mellitus (T2DM), hypertension, hyperlipidemia and coronary heart disease. IR is a multi-factorial etiology disease regulated by various mechanisms. Obesity, a significant public health problem worldwide, is considered as a high-risk factor to induce IR and T2DM. Thus, inhibiting obesity-induced IR is one of the key therapeutic strategies to improve T2DM. Natural products from herbs and nutraceuticals have shown promising efficacy on obesity-induced IR and T2DM owing to its multi-component and multi-target characteristics. However, the active constituents and the action mechanisms are still not yet well elucidated. In this review, the natural constituents with protective capacities against IR were summarized. The active constituents include crude extracts such as polyphenols, saponins, alkaloids, terpenoids, phenylpropanoids, as well as monomeric compounds such as curcumin, berberine, capsaicin, naringenin, quercetin, betaine and isoliquiritigenin. The action mechanisms of these active constituents could be mainly divided into 4 categories: ameliorating metabolic abnormality, inhibiting inflammation, reversing gut microbiota dysbiosis, regulating micro RNAs (miRNAs). Their precise roles in regulating PI3K/AKT, AMPK, PPARs, SIRT1 and NF-κB pathways, brown adipose tissue activity and white adipose tissue browning, NLRP3 inflammasome activity, gut microbiota structure and composition and miRNA expression were discussed. The sources, chemical structures, experimental models, pharmacological effects and action targets of these active constituents were also summarized. This review provides new active constituents, therapeutic targets and reasoning for the clinical application of herbs and nutraceuticals in the treatment of IR and T2DM.

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Diphyllin Improves High-Fat Diet-Induced Obesity in Mice Through Brown and Beige Adipocytes

Cited by 11 publications

References 64 publications

Microfibrillar-associated protein 5 suppresses adipogenesis by inhibiting essential coactivator of PPARγ

Microfibrillar-associated protein 5 suppresses adipogenesis by inhibiting essential coactivator of PPARγ

Reveals of candidate active ingredients in Justicia and its anti-thrombotic action of mechanism based on network pharmacology approach and experimental validation

New insights of active constituents from herbs and nutraceuticals and their mechanisms in obesity-induced insulin resistance

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