Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica

Abstract

Limnophila aromatica is commonly used as a spice and a medicinal herb in Southeast Asia. In this study, water and various concentrations (50%, 75%, and 100%) of methanol, ethanol, and acetone in water were used as solvent in the extraction of L. aromatica. The antioxidant activity, total phenolic content, and total flavonoid content of the freeze-dried L. aromatica extracts were investigated using various in vitro assays. The extract obtained by 100% ethanol showed the highest total antioxidant activity, reducing power and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity. The same extract also exhibited the highest phenolic content (40.5 mg gallic acid equivalent/g of defatted L. aromatica) and the highest flavonoid content (31.11 mg quercetin equivalent/g of defatted L. aromatica). The highest extraction yield was obtained by using 50% aqueous acetone. These results indicate that L. aromatica can be used in dietary applications with a potential to reduce oxidative stress.

Chemical Composition, Toxicity and Antifungal Activities of Megaphrynium macrostachyum (K. Schum) Leaf Extract against Foodborne Fungi

“…The percentage yield of the aqueous extract was the highest among the four solvents analyzed and this corroborated earlier work by Do et al (2014) on the extraction yield of Limnophila aroamtica and this is also in agreement with the works of Dellavallle et al (2011) and Nurain et al (2013) that identified aqueous extracts with higher yield compared with other test solvents. Francois et al (2015) in their study showed that aqueous extracts had higher yield compared with the ethanol, benzene and chloroform extracts and this suggests a higher proportion of water-soluble plant components.…”

Free radicals scavenging activity and reducing power of two Algerian Sahara medicinal plants extracts

“…High solubility of phenols in polar solvents provides high concentration of these compounds in the extracts obtained using polar solvents for the extraction. The concentration of flavonoids in plant extracts depends on the polarity of solvents used in the extract preparation [14,15] . The results of [16] have shown that the polyphenol contents of the methanol extract that is greater than the aqueous extract although the study is carried out on the Capparis spinosa L. Therefore, many studies demonstrated that polar solvents give higher yields than apolar solvents, since the polar solvents have the ability to spread within the plant powder, reaching the vegetable matrix and therefore recover the possible metabolites.…”

Potencial alelopático do extrato etanólico de Anacardium humile (cajuzinho-do-cerrado) na germinação e formação de plântulas de alface, tomate e fedegoso

“…A análise foi executada por interpolação da absorbância das amostras contra uma curva de calibração (y = 0,1350x -185,00; R² = 0,99), construída com padrões de ácido gálico (GAE 10 a 300 μg mL -1 ), e os resultados expressos em equivalentes de ácido gálico (mg/g). Para quantificação dos flavonoides, a quercetina (QE = 0,5 mg mL -1 ) foi o padrão para construir a curva de calibração nas concentrações de 0,04; 0,2; 0,4; 2; 4; 8; 12; 16; e, 20 μg mL -1 (y = 0,06 x + 678,9 R² = 0,90), com análises realizadas por espectrofotometria no comprimento de onda de 420 nm, em cubetas de quartzo, com seu conteúdo expresso em equivalentes de quercetina (mg/g) (Do et al 2014).…”