Effect of systemic pH on pHi and lactic acid generation in exhaustive forearm exercise

Hood, Virginia L.; Schubert, Conrad von; Keller, Ulrich; Müller, S.

doi:10.1152/ajprenal.1988.255.3.f479

Cited by 46 publications

(47 citation statements)

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“…A decrease in pH is observed following inflammation, hematomas and isometric exercise (Hood et al, 1988;Issberner et al, 1996;Pan et al, 1988;Revici et al, 1949). In fact, there is a positive correlation between pain and local acidity (Issberner et al, 1996).…”

Section: Acid Pain and Asicsmentioning

confidence: 99%

“…A decrease in pH is observed following inflammation, hematomas and isometric exercise (Hood et al, 1988;Issberner et al, 1996;Pan et al, 1988;Revici et al, 1949), and decreases in pH in muscle produce pain in humans (Issberner et al, 1996). Peripheral initiators of muscle pain are virtually unknown, but are likely key to development of chronic pain after muscle insult.…”

Section: Introductionmentioning

confidence: 99%

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ASIC3 in muscle mediates mechanical, but not heat, hyperalgesia associated with muscle inflammation

Sluka

Radhakrishnan

Benson

et al. 2007

Pain

166

157

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Peripheral initiators of muscle pain are virtually unknown, but likely key to development of chronic pain after muscle insult. The current study tested the hypothesis that ASIC3 in muscle is necessary for development of cutaneous mechanical, but not heat hyperalgesia induced by muscle inflammation. Using mechanical and heat stimuli, we assessed behavioral responses in ASIC3−/− and ASIC3+/+ mice after induction of carrageenan muscle inflammation. ASIC3−/−mice did not develop cutaneous mechanical hyperalgesia after muscle inflammation when compared to ASIC3+/ + mice; heat hyperalgesia developed similarly between groups. We then tested if the phenotype could be rescued in ASIC3−/− mice by using a recombinant herpes virus vector to express ASIC3 in skin (where testing occurred) or muscle (where inflammation occurred). Infection of mouse DRG neurons with ASIC3-encoding virus resulted in functional expression of ASICs. Injection of ASIC3-encoding virus into muscle or skin of ASIC3−/− mice resulted in ASIC3 mRNA in DRG and protein expression in DRG and the peripheral injection site. Injection of ASIC3-encoding virus into muscle, but not skin, resulted in development of mechanical hyperalgesia similar to that observed in ASIC3+/+ mice. Thus, ASIC3 in primary afferent fibers innervating muscle is critical to development of hyperalgesia that results from muscle insult.

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Section: Acid Pain and Asicsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

ASIC3 in muscle mediates mechanical, but not heat, hyperalgesia associated with muscle inflammation

Sluka

Radhakrishnan

Benson

et al. 2007

Pain

166

157

View full text Add to dashboard Cite

show abstract

“…Low pH can elicit pain, and decreases in tissue pH occur in a number of biological settings (Hood et al, 1988;Pan et al, 1988;Issberner et al, 1996;Reeh and Steen, 1996). In humans, intramuscular infusion of acidic buffer causes painful sensations and increased sensitivity to mechanical stimulation (Steen and Reeh, 1993;Issberner et al, 1996).…”

Section: Muscle-derived Mechanical Secondary Hyperalgesiamentioning

confidence: 99%

Neurotrophin-3 Reverses Chronic Mechanical Hyperalgesia Induced by Intramuscular Acid Injection

Gandhi

Ryals²,

Wright³

2004

J. Neurosci.

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Injection of acid into the gastrocnemius muscle results in a persistent, mechanical hyperalgesia of the hindpaw (Sluka et al., 2001). Here, the ability of neurotrophins to alter the development of this secondary hyperalgesia was assessed using transgenic mice and exogenous neurotrophin administration. Acid-induced hyperalgesia was measured in wild-type and transgenic mice that overexpress neurotrophin-3 (NT-3) in muscle (myo/NT-3 mice). Mechanical and thermal sensitivity of the hindpaws were assessed after injections of acidic saline, pH 4, into the right medial gastrocnemius. Wild-type mice exhibited mechanical but not thermal hyperalgesia in both paws 1 d after acid injection. In contrast, myo/NT-3 mice developed a transient mechanical hyperalgesia in both paws that disappeared by 2-3 d. The reversal of hyperalgesia in myo/NT-3 mice could be mimicked by intramuscular administration of exogenous NT-3 to acid-injected mice but not by other neurotrophins. The route of NT-3 administration appears critical, because intrathecal or intraperitoneal delivery were ineffective. The hyperalgesia could only be reversed by NT-3 treatment concurrent with acid injection and not after the emergence of hyperalgesia. The acid-induced hyperalgesia did not redevelop after the termination of NT-3 treatment, suggesting that NT-3 permanently reversed the hyperalgesia. Consistent with the behavioral data, paw palpation of acid-injected mice significantly increased Fos expression in the spinal cord of wild-type but not myo/NT-3 or NT-3-injected mice. The attenuation of hyperalgesia suggests that NT-3 may be a modulator of muscle-derived pain, and NT-3 may suppress events that lead to secondary hyperalgesia triggered by insult to muscle afferents.

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“…Energy supply, under anaerobic conditions, is provided by both PCr hydrolysis and anaerobic glycolysis, the latter resulting in lactate acidosis. In isolated muscle, the external acidosis has been shown to decrease, and alkalosis to increase, the net lactic acid production (Hood et al, 1988). These findings have been mainly ascribed to the pHi dependence of the phosphofructokinase enzyme (Trivedi and Danforth, 1966).…”

Section: Proton Loading and Effluxmentioning

confidence: 99%

“…In general, owing to the presence of pH-sensitive ionizing groups, enzymes are active only over a limited range of pH with a definite optimum. Moreover, extracellular acidosis has been shown to play a key role in metabolic processes (Hood et al, 1988). Also, severe hypoxia and anoxia may modulate the ATP demand by the cell (Hochachka, 1986a,b).…”

mentioning

confidence: 99%

Effects of temperature and extracellular pH on metabolites: kinetics of anaerobic metabolism in resting muscle by 31P- and 1H-NMR spectroscopy

Vezzoli

Gussoni

Greco

et al. 2003

Journal of Experimental Biology

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Muscle metabolism is susceptible to a variety of stressing agents affecting particularly the overall rate of energy turnover. As is well known, temperature is a key environmental determinant of the activity of cellular multienzyme systems, such as muscle. In fact, this variable determines the kinetics and flux throughout biochemical pathways, particularly metabolic rates. Another key environmental determinant of cellular multienzyme system activity is intracellular pH. In general, owing to the presence of pH-sensitive ionizing groups, enzymes are active only over a limited range of pH with a definite optimum. Moreover, extracellular acidosis has been shown to play a key role in metabolic processes (Hood et al., 1988). Also, severe hypoxia and anoxia may modulate the ATP demand by the cell (Hochachka, 1986a,b). Skeletal muscle is known to be one of the most hypoxia-tolerant tissues and, in O2-limiting conditions, can survive by means of a metabolic depression, thereby saving substrates and reducing deleterious end-product formation.Ectothermic vertebrates, such as the frog, that normally experience rapid and large variations in body temperature (T) and consequent acid-base state changes, based on physicochemical adaptations (Boutilier, 2001), appear to tolerate such changes with minimal metabolic disturbances. The effects of temperature variations on acid-base balance regulation mechanisms have been well documented (Reeves, 1969). The 'in vivo' blood pH of most studied ectothermic species decreases as temperature increases with a slope close to -0.018·pH·units·°C -1 (Malan et al., 1976). It has been suggested that ∆pH/∆T changes of such an extent can keep the histidine groups of proteins at an almost constant dissociation state (alphastat hypothesis; Reeves, 1972 Environmental stress, such as low temperature, extracellular acidosis and anoxia, is known to play a key role in metabolic regulation. The aim of the present study was to gain insight into the combined temperature-pH regulation of metabolic rate in frog muscle, i.e. an anoxia-tolerant tissue. The rate of exergonic metabolic processes occurring in resting isolated muscles was determined at 15°C and 25°C as well as at extracellular pH values higher (7.9), similar (7.3) and lower (7.0) than the physiological intracellular pH. 31 P and 1 H nuclear magnetic resonance spectroscopy high-resolution measurements were carried out at 4.7·T in isolated frog (Rana esculenta) gastrocnemius muscle during anoxia to assess, by means of reference compounds, the concentration of all phosphate metabolites and lactate. Intra-and extracellular pH was also determined.In the range of examined temperatures (15-25°C), the temperature dependence of anaerobic glycolysis was found to be higher than that of PCr depletion (Q 10 =2.3). High-energy phosphate metabolism was confirmed to be the initial and preferential energy source. The rate of phosphocreatine hydrolysis did not appear to be affected by extracellular pH changes. By contrast, independent of the intracellular pH value, at ...

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Effect of systemic pH on pHi and lactic acid generation in exhaustive forearm exercise

Cited by 46 publications

References 0 publications

ASIC3 in muscle mediates mechanical, but not heat, hyperalgesia associated with muscle inflammation

ASIC3 in muscle mediates mechanical, but not heat, hyperalgesia associated with muscle inflammation

Neurotrophin-3 Reverses Chronic Mechanical Hyperalgesia Induced by Intramuscular Acid Injection

Effects of temperature and extracellular pH on metabolites: kinetics of anaerobic metabolism in resting muscle by 31P- and 1H-NMR spectroscopy

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