Effects of betaine on viability, apoptosis, function protein expression and oxidative status in heat-stressed IEC-6 cells

Sun, Shiang; Yin, Qirun; Li, Bing; Deng, Yafei; Li, Jing; Xiong, Yi; He, Suqin

doi:10.1016/j.jtherbio.2022.103348

Cited by 3 publications

(1 citation statement)

References 26 publications

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“…20 In recent years, studies on the application of betaine as a drug have been discussed in terms of its action at the molecular level or its role as a surfactant, whereas studies on its formulation are scarce. 21,22 The only study of betaine as a preparation is in tablets for the treatment of anti-hyperhomocysteinemia diseases. 23 There are no published reports on the preparation of betaine for injection or for the use of betaine via nebulised inhalation (Inh.)…”

Section: Introductionmentioning

confidence: 99%

Preparation of betaine injection and its therapeutic effect in pulmonary arterial hypertension

Che,

Xia,

Liu

et al. 2023

Basic Clin Pharma Tox

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Pulmonary arterial hypertension (PAH) is a life‐threatening disease characterised by elevated pulmonary pressure, right ventricular failure (RVF) and ultimately death. Aggressive treatment of RVF is considered an important therapeutic strategy to treat PAH. Previous studies have indicated that betaine may be may a promising therapeutic approach for PAH‐induced RVF. Therefore, in this study, betaine solution for injection was prepared and characterised using various techniques. The therapeutic efficacy of three different methods of administration (intragastric, nebulised inhalation and intravenous injection) were comprehensively evaluated in terms of pharmacokinetics, tissue distribution and pharmacodynamics. The pharmacokinetic results demonstrated that betaine injection administered via nebulised inhalation significantly prolonged betaine's half‐life and increased its internal circulation time compared to the intragastric and intravenous routes. Biodistribution experiments verified that the betaine formulation accumulated in the lung tissue when administered via inhalation. The results of the pharmacodynamic analysis further confirmed that right ventricular systolic pressure, mean pulmonary artery pressure and right ventricular hypertrophy index increased in the model group and that inhaled betaine suppressed these pathological changes to a level comparable to those observed in the control group. Taken together, these results indicate that betaine administered by inhalation is a promising strategy for the treatment of PAH‐induced RVF.

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Section: Introductionmentioning

confidence: 99%

Preparation of betaine injection and its therapeutic effect in pulmonary arterial hypertension

Che,

Xia,

Liu

et al. 2023

Basic Clin Pharma Tox

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Protective influence of supplementary betaine against heat stress by regulating intestinal oxidative status and microbiota composition in broiler chickens

Al Sulaiman,

Abudabos,

Alhotan

2023

Int J Biometeorol

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Tributyrin displayed the potential nutrient mechanism by improving the growth of rumen bacteria of weaned lambs rather than via releasing butyric acid

Wang,

Chen,

et al. 2024

Preprint

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Background Tributyrin (TB), a feed additive, has been widely proved to display a benefit in stimulating the gastrointestinal tract development of livestock. For a long time, the benefit of TB is regarded as the prodrug of butyric acid. However, more and more studies recently showed that supplementing TB could enhance gastrointestinal microbiotas accompanied with the benefit. Here, we investigated the potential nutrient mechanism of TB based on in vitro ICE-6 intestinal epithelial cell culture in the absence of bacteria and in vivo experiment with involvement of rumen bacteria. Results In vitro experiment showed that butyric acid linearly increased both the late and early apoptotic ICE-6 intestinal epithelial cells, but similar effects were not observed in the same concentrations of TB. In vivo experiment showed that supplementing TB increased dry matter intake of lambs and daily retentions of dietary serine, cysteine and methionine. Besides, lambs fed TB had higher faecal excretions of C12:0, C13:0, C18:2n6c, C18:3n3 and C20:0 but lower C16:1n9c, C17:0 and C18:1n9c compared with that of lambs provided no TB. The correlation analysis showed that daily retentions of dietary serine, methionine and cysteine were positively correlated with the relative abundances of ruminal Fibrobacter, Butyrivibrio, Bifidobacterium and Firmicutes, respectively. In addition, the relative abundance of rumen Clostridium was positively correlated with the daily decreases of C6:0, C18:1n9c, C18:2n6c and the daily increases of C13:0, C15:0 and C18:0, respectively. Meanwhile, the relative abundance of rumen Fibrobacter was positively correlated with the daily decreases of C6:0, C16:0, C18:1n9c, C18:2n6c, C18:3n3, C20:0, C22:0, C24:0 while was negatively correlated with the daily increases of C14:0, C15:0, C16:1n9c and C18:0, respectively. Furthermore, the relative abundance of rumen Butyrivibrio was negatively correlated with the daily increases of C14:0, C16:1n9c, C17:0 while was positively correlated with the daily decreases of C16:0, respectively. Conclusions Our both in vitro and in vivo experiments revealed that supplementing TB could display the nutrient mechanism by improving the growth of rumen bacteria rather than via releasing butyric acid.

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Effects of betaine on viability, apoptosis, function protein expression and oxidative status in heat-stressed IEC-6 cells

Cited by 3 publications

References 26 publications

Preparation of betaine injection and its therapeutic effect in pulmonary arterial hypertension

Preparation of betaine injection and its therapeutic effect in pulmonary arterial hypertension

Protective influence of supplementary betaine against heat stress by regulating intestinal oxidative status and microbiota composition in broiler chickens

Tributyrin displayed the potential nutrient mechanism by improving the growth of rumen bacteria of weaned lambs rather than via releasing butyric acid

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