Efficient 2,3-butanediol production from whey powder using metabolically engineered Klebsiella oxytoca

Meng, Wensi; Cao, Menghao; Zhang, Wen; Lü, Chuanjuan; Yang, Chunyu; Gao, Chao; Xu, Ping; Ma, Cuiqing

doi:10.1186/s12934-020-01420-2

Cited by 29 publications

(20 citation statements)

References 43 publications

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“…To evaluate the influence of pH on SSF of liquefied cassava (340 g L −1 ) by K. oxytoca, the cassava solutions adjusted at various pHs (5.5, 6.0, and 6.5) were allowed to ferment at the optimal temperature of 37 °C. 29 Fig. 5 shows the profiles of residual glucose and 2,3-BDO production in the cassava medium with various pHs.…”

Section: Effect Of Ph On Saccharification and Ssfmentioning

confidence: 99%

Integrated process development for the efficient and industrial production of 2,3‐butanediol and bioethanol using dried cassava chips as commercial biomass

Moon¹,

Chung²,

Min

2021

J of Chemical Tech & Biotech

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BACKGROUND: For the efficient use of cassava as industrial material in the fermentation business, an integrated process was developed to produce 2,3-butanediol (2,3-BDO) and bioethanol (BE) by a highly efficient and economical method. After separating the liquefied cassava into a solid and a liquid part, the liquid part was used to produce 2,3-BDO, and the solid part was then used to produce BE. RESULTS:The integrated process efficiently fermented 1000 g of dried cassava to 288 g of 2,3-BDO (120.1 g L −1 titer and 2.39 L fermented broth) and 57 g of BE (38.4 g L −1 titer and 1.49 L fermented broth). Also, fermentable sugar of cassava was 100% available for this process, and the byproduct of BE production, condensed distillers solubles (CDS), was used as a potential nutrient source for the economical production of 2,3-BDO.CONCLUSION: An integrated process with simultaneous saccharification and fermentation (SSF), pH shift, and rpm shift was developed to enhance 2,3-BDO production and to reduce the production cost of 2,3-BDO and BE. The strategy of employing cassava as a starchy biomass-based biorefinery resource for 2,3-BDO and BE production was successfully performed.

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Section: Effect Of Ph On Saccharification and Ssfmentioning

confidence: 99%

Integrated process development for the efficient and industrial production of 2,3‐butanediol and bioethanol using dried cassava chips as commercial biomass

Moon¹,

Chung²,

Min

2021

J of Chemical Tech & Biotech

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“…Recent studies like those performed by Meng et al. (2020) and Alvarez‐Guzmán et al. (2020) used whey powder to obtain 2,3‐BD.…”

Section: Introductionmentioning

confidence: 99%

Fermentation of whey and its permeate using a genetically modified strain of Escherichia coli K12 MG1655 to produce 2,3‐butanediol

Fernández‐Gutiérrez

Veillette

Ramírez

et al. 2021

Environmental Quality Mgmt

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Whey is generated during cheese manufacturing, and permeate whey is obtained after whey deproteinization. Both effluents contain lactose, which can cause environmental issues if they are released into the environment. The aim of the present study was the valorization of lactose contained in whey and permeate whey via fermentation into 2,3‐butanediol (2,3‐BD) with genetically modified strain of Escherichia coli K12 MG1655. Both effluents were first fermented at various dilution ratios with the culture medium M9 (50:50, 75:25, and 100:0, v/v). The fermentation of undiluted effluents produced the highest 2,3‐BD yield (0.43 g/g lactose) at 72 h. Afterwards, the effects of initial pH, inoculum size and agitation rate on 2,3‐BD yield in flask fermentation of undiluted effluents were studied. The agitation rates of 50 and 200 rpm resulted in lower 2,3‐BD yields compared with 100 rpm (67% lower). The effect of aeration (2.5 vvm) on 2,3‐BD yield was tested in a 2 L bioreactor, where a 2,3‐BD yield of 0.40 g/g lactose was obtained after 24 h. In the present study, it was demonstrated that both whey and permeate whey can be used to produce 2,3‐BD, reaching near 80% of the theoretical yield after 24 h of fermentation.

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“…Klebsiella oxytoca ha sido utilizada con fines biotecnológicos en diversas áreas, presenta potencial biorremediador, pues degrada hidrocarburos [11], compuestos orgánicos hidrofóbicos [12], sustancias tóxicas como el cianuro [13], así como insecticidas organoclorados [14], nitrilos [15], ácidos benzoicos [16], colorantes azoicos [17] y el fármaco diclofenaco [18]. Además, se ha reportado la producción de bioelectricidad en conjunto con la degradación de ácidos grasos [19], ha sido capaz de llevar a cabo la desulfuración termofílica de dibenzotiofenos [20], entre otras muchas aplicaciones [21] [22] [23].…”

Section: Introductionunclassified

Optimización de las condiciones de crecimiento de la bacteria endófita Klebsiella oxytoca en matraz y en biorreactor

Schmidt-Durán

Chacón-Cerdas

2021

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Las bacterias endófitas son aquellas que viven en el interior de las células o los tejidos de plantas superiores sin causar daño. Klebsiella oxytoca es una bacteria endófita promotora del crecimiento vegetal y con efecto biocontrolador, razón por la cual, el objetivo del presente estudio fue determinar las condiciones óptimas de crecimiento de esta bacteria, en matraz y en biorreactor, obtenida de plantas de higo (Ficus carica) en estudios anteriores. Para esto, se evaluó el crecimiento de la biomasa de K. oxytoca en caldo nutritivo bajo diferentes condiciones de agitación y temperatura en matraz, determinando que las condiciones a 200 rpm y 30 °C fueron las que produjeron una mayor biomasa. Posteriormente, utilizando medio caldo nutritivo en el biorreactor de 3,7 L, se determinó que una agitación de 500 rpm presenta valores de KLa más altos y tiempos de fermentación menores que si se trabaja a 750 rpm. Además, bajo una agitación de 500 rpm, se determinó que medios basados en residuos agroindustriales pueden funcionar como fuentes de crecimiento alternativas para este microorganismo. Por último, el tratamiento de caldo nutritivo a 500 rpm fue modelado mediante el modelo logístico y la ecuación de Pirt, obteniendo, en ambos casos, coeficientes de determinación superiores a 0,95.

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Efficient 2,3-butanediol production from whey powder using metabolically engineered Klebsiella oxytoca

Cited by 29 publications

References 43 publications

Integrated process development for the efficient and industrial production of 2,3‐butanediol and bioethanol using dried cassava chips as commercial biomass

Integrated process development for the efficient and industrial production of 2,3‐butanediol and bioethanol using dried cassava chips as commercial biomass

Fermentation of whey and its permeate using a genetically modified strain of Escherichia coli K12 MG1655 to produce 2,3‐butanediol

Optimización de las condiciones de crecimiento de la bacteria endófita Klebsiella oxytoca en matraz y en biorreactor

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