Environmental Enrichment Alters Splenic Immune Cell Composition and Enhances Secondary Influenza Vaccine Responses in Mice

Gurfein, Blake T.; Davidenko, Olga; Premenko-Lanier, Mary; Milush, Jeffrey M.; Acree, Michael; Dallman, Mary F.; Touma, Chadi; Palme, Rupert; York, Vanessa A.; Fromentin, Gilles; Darcel, Nicolas; Nixon, Douglas F.; Hecht, Frederick M.

doi:10.2119/molmed.2013.00158

Cited by 28 publications

(24 citation statements)

References 49 publications

(52 reference statements)

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“…Thus the subpopulation distribution is much closer to C57Bl/6 mice 22 weeks of age (Pinchuk and Filipov, ). Some other variations are probably due to the difference of species or age (Avitsur et al, ; Gurfein et al, ). NK cells represent only 9% of total spleen cell population, in compliance with prior results (Avitsur et al, ), so we decided to purify this cell fraction.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of Lytic Activity by Leptin Is Independent From Lipid Rafts in Murine Primary Splenocytes

et al. 2016

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Leptin, a pleiotropic adipokine, is known as a regulator of food intake, but it is also involved in inflammation, immunity, cell proliferation, and survival. Leptin receptor is integrated inside cholesterol-rich microdomains called lipid rafts, which, if disrupted or destroyed, could lead to a perturbation of lytic mechanism. Previous studies also reported that leptin could induce membrane remodeling. In this context, we studied the effect of membrane remodeling in lytic activity modulation induced by leptin. Thus, primary mouse splenocytes were incubated with methyl-β-cyclodextrin (β-MCD), a lipid rafts disrupting agent, cholesterol, a major component of cell membranes, or ursodeoxycholic acid (UDCA), a membrane stabilizer agent for 1 h. These treatments were followed by splenocyte incubation with leptin (absence, 10 and 100 ng/ml). Unlike β-MCD or cholesterol, UDCA was able to block leptin lytic induction. This result suggests that leptin increased the lytic activity of primary spleen cells against syngenic EO771 mammary cancer cells independently from lipid rafts but may involve membrane fluidity. Furthermore, natural killer cells were shown to be involved in the splenocyte lytic activity. To our knowledge it is the first publication in primary culture that provides the link between leptin lytic modulation and membrane remodeling. J. Cell. Physiol. 232: 101-109, 2017. © 2016 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 99%

Enhancement of Lytic Activity by Leptin Is Independent From Lipid Rafts in Murine Primary Splenocytes

et al. 2016

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“…Therefore, an increase in these cells due to transport stress was expected. It has been suggested that environmental enrichment did not change (Olsson et al 2010), improved (Gurfein et al 2014), or suppressed (Hutchinson et al 2005) CD4+ and CD8+ cell proportions in lymphoid tissues of rodents. It has also been reported that environmental enrichment effectively improved the immune system in laying hens (Moe et al 2010) and Japanese quails (Nazar & Marin 2011b), but it has not been examined whether enrichment affected CD4+ and CD8+ cells in these studies.…”

Section: Discussionmentioning

confidence: 99%

The effects of environmental enrichment and transport stress on the weights of lymphoid organs, cell‐mediated immune response, heterophil functions and antibody production in laying hens

Matur

Akyazı

Eraslan

et al. 2015

Animal Science Journal

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The effects of environmental enrichment and transport stress on the immune system were investigated in laying hens. A total of 48 1-day-old chickens were used, half of the chickens were reared in conventional cages (RCC) and the rest in enriched cages (REC). Transport stress was applied in the 17th week. Liver weight decreased, spleen and bursa of Fabricius weights, white blood cell count, CD4+ and CD8+ cell proportions increased due to the transport. Environmental enrichment significantly increased antibody production and tended to increase monocyte percentage and CD8+ cell proportion. The effect of transport on, heterophil (H) and lymphocyte (L) percentages was not significant in RCC chickens. While heterophil percentage and H:L ratio increased, lymphocyte percentage decreased in REC chickens subjected to transport. Transport stress increased heterophil functions both in REC and RCC chickens, but the increase was higher in REC hens than in RCC hens. In conclusion, although environmental enrichment did not neutralize the effect of transport on lymphoid organs, it activated the non-specific immune system, cellular and the humoral branches of the specific immune system by increasing heterophil functions, CD8+ cells and antibody production, respectively. Therefore, environmental enrichment suggested for improving animal welfare may also be beneficial to improve the immune system of birds exposed to stress.

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“…Numerous studies have shown that animals living in captive environments are generally abnormal and unhealthy, as such environments change their behavior as well as immune, nervous, and endocrine functionality. Examples include their altered response to infection (Gurfein et al, 2014), altered immune response (Beura et al, 2016;Messmer et al, 2014), increased rates of obesity, Type ii diabetes, high blood pressure, and premature death (Martin et al, 2010), altered brain development (Bennett et al, 1964;Kempermann, Kuhn and Gage, 1997;Lewis et al, 2006;Rosenzweig and Bennett, 1969;Rosenzweig et al, 1962), decreased strength and endurance (During et al, 2015), altered sleep, activity patterns, and blood pressure (Martire et al, 2012), altered growth rates (Serrat, King and Lovejoy, 2008), altered organ development, metabolic, growth, and reproduction rates and behavior (Gordon, 2012), and enhanced tumor growth (Cao et al, 2010;Li et al, 2015). As such, untreated control animals do not represent healthy individuals, since they are metabolically abnormal (Martin et al, 2010).…”

Section: 3mentioning

confidence: 99%

Refinement on the Way Towards Replacement: Are We Doing What We Can?

Herrmann¹

2019

Animal Experimentation: Working Towards a Paradigm Change

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Environmental Enrichment Alters Splenic Immune Cell Composition and Enhances Secondary Influenza Vaccine Responses in Mice

Cited by 28 publications

References 49 publications

Enhancement of Lytic Activity by Leptin Is Independent From Lipid Rafts in Murine Primary Splenocytes

Enhancement of Lytic Activity by Leptin Is Independent From Lipid Rafts in Murine Primary Splenocytes

The effects of environmental enrichment and transport stress on the weights of lymphoid organs, cell‐mediated immune response, heterophil functions and antibody production in laying hens

Refinement on the Way Towards Replacement: Are We Doing What We Can?

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