(−)Epigallocatechin gallate hampers collagen destruction and collagenase activation in ultraviolet-B-irradiated human dermal fibroblasts: Involvement of mitogen-activated protein kinase

Bae, Ji‐Young; Choi, Jung‐Suk; Choi, Yong‐Kook; Shin, Sungwon; Kang, Sang‐Wook; Han, Seoung Jun; Kang, Young‐Hee

doi:10.1016/j.fct.2007.09.112

Cited by 137 publications

(123 citation statements)

References 35 publications

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“…15) Numerous studies have shown that polyphenols, such as epigallocatechin gallate (EGCG), 16) resveratrol (trans-3,5,4′-trihydroxystilbene), 17) and quercetin (3,5,7,3′,4′-pentahydroxyflavon), 18) prevent UVinduced skin ageing.…”

Section: 11)mentioning

confidence: 99%

“…In recent years, naturally occurring compounds, such as phenolic acids, flavonoids, and high molecular weight polyphenols, have gained considerable attention as beneficial protective agents. 15) Numerous studies have shown that polyphenols, such as epigallocatechin gallate (EGCG), 16) resveratrol (trans-3,5,4′-trihydroxystilbene), 17) and quercetin (3,5,7,3′,4′-pentahydroxyflavon), 18) prevent UVinduced skin ageing.…”

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confidence: 99%

“…13,14) Antioxidants are regarded as promising agents that reduce such oxidative stress. In recent years, naturally occurring compounds, such as phenolic acids, flavonoids, and high molecular weight polyphenols, have gained considerable attention as beneficial protective agents.15) Numerous studies have shown that polyphenols, such as epigallocatechin gallate (EGCG), 16) resveratrol (trans-3,5,4′-trihydroxystilbene), 17) and quercetin (3,5,7,3′,4′-pentahydroxyflavon), 18) prevent UVinduced skin ageing.Passion fruit (Passiflora edulis SimS.) is a vine species of the passion flower family (Passifloraceae) indigenous to the tropical regions of America and is known as a medicinal herb.…”

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The Protective Effects of Piceatannol from Passion Fruit (<i>Passiflora edulis</i>) Seeds in UVB-Irradiated Keratinocytes

Maruki‐Uchida

Kurita

Sugiyama

et al. 2013

Biological & Pharmaceutical Bulletin

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The use of naturally occurring botanicals with substantial antioxidant activity to prevent photoageing is receiving increasing attention. We have previously identified piceatannol and scirpusin B, which is a dimer of piceatannol, as strong antioxidants that are present in passion fruit (Passiflora edulis) seeds. In the present study, the effects of passion fruit seed extract, piceatannol, and scirpusin B on human keratinocytes were investigated. The passion fruit seed extract and piceatannol upregulated the glutathione (GSH) levels in keratinocytes in a dose-dependent manner, indicating that piceatannol is an active component of the passion fruit seed extract in keratinocytes. The pretreatment with piceatannol also supressed the UVB-induced generation of reactive oxygen species (ROS) in the keratinocytes. In addition, the transfer of the medium from the UVBirradiated keratinocytes to non-irradiated fibroblasts enhanced matrix-metalloproteinase (MMP)-1 activity, and this MMP-1 induction was reduced when the keratinocytes were pretreated with piceatannol. These results suggest that piceatannol attenuates the UVB-induced activity of MMP-1 along with a reduction of ROS generation in keratinocytes. Thus, piceatannol and passion fruit seed extract containing high amounts of piceatannol are potential anti-photoageing cosmetic ingredients.Key words piceatannol; passion fruit seed; keratinocyte; ultraviolet; reactive oxygen species; matrix-metalloproteinase Solar UV irradiation damages human skin causing it to age prematurely, a process referred to as photoageing, and to develop the characteristics of thickened epidermis and increased melanogenesis.1-3) In contrast, intrinsic (chronological) ageing is characterised by skin atrophy accompanied by a loss of elasticity and reduced metabolic activity. [4][5][6][7][8] A hallmark of photoageing is the disorganisation of collagen, the major structural component of the skin. Collagen is produced mainly by dermal fibroblasts and is degraded by the matrix metalloproteinases (MMPs) that are secreted by various cells, including keratinocytes, fibroblasts, and inflammatory cells.2,8,9) UV irradiation, particularly UVB (290-320 nm) and UVA (315-400 nm), induces the expression of matrix metalloproteinase-1 (MMP-1) in the fibroblasts, which is mainly responsible for the degradation of the dermal collagen in the ageing process of human skin. Therefore, UV-induced MMP-1 is considered to contribute substantially to the connective tissue damage that causes severe collagen deficiency and wrinkling during photoageing.2,8) Although keratinocytes do not produce MMP-1 in response to UVB irradiation, damaged epidermal keratinocytes indirectly play important roles in the release of MMP-1. It has been reported that the culture medium of UVB-irradiated keratinocytes stimulates MMP-1 release from fibroblasts more efficiently than the direct irradiation of the fibroblasts. 10,11)The primary mechanism by which UV irradiation initiates molecular responses in human skin is through the photochromic genera...

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Section: 11)mentioning

confidence: 99%

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The Protective Effects of Piceatannol from Passion Fruit (<i>Passiflora edulis</i>) Seeds in UVB-Irradiated Keratinocytes

Maruki‐Uchida

Kurita

Sugiyama

et al. 2013

Biological & Pharmaceutical Bulletin

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“…By hydrogen bonding and hydrophobic interactions with the collagenase, EGCG might lead to conformational changes or to masking of the catalytic region of MMP-2 24,25 . EGCG can also inhibit the activation of MMP-8 26 , which in turn was shown to affect the remineralisation of artificially demineralised dentin 27 .…”

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Chlorhexidine and green tea extract reduce dentin erosion and abrasion in situ

Magalhães

Wiegand

Ríos

et al. 2009

Journal of Dentistry

126

119

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OBJECTIVES: This in situ/ex vivo study aimed to analyse the impact of possible MMPinhibitors (chlorhexidine and green tea extract) on dentin wear induced by erosion or erosion plus abrasion. METHODS: Twelve volunteers took part in this cross-over and double-blind study performed in 4 phases of each 5 days. Bovine dentin samples were worn in palatal appliances and subjected to extraoral erosion (4 times/day, Coca-Cola, 5 min) or erosion plus abrasion (2 times/day, fluoride-free toothpaste and electrical toothbrush, 15s/sample). Immediately after each erosion, the appliances were reinserted in the mouth and the oral cavity was rinsed for 60s with: 250 ppm F solution (SnF(2)/AmF, pH 4.5, Meridol-Gaba, Switzerland), 0.12% chlorhexidine digluconate (0.06% chlorhexidine, pH 6.0, Periogard-Colgate, Brazil), 0.61% green tea extract solution (OM24, 100% Camellia Sinensis leaf extract, catechin concentration: 30+/-3%, pH 7.0, Omnimedica, Switzerland) or deionized water (pH 6.0, control). Dentin loss was assessed by profilometry (microm). The data were analysed by two-way repeated measures ANOVA and Bonferroni post hoc test. RESULTS: There was a significant difference between the conditions (EroxEro+Abr, p<0.001) and among the solutions (p<0.001). All solutions (F: 1.42+/-0.34; 1.73+/-0.50, chlorhexidine: 1.15+/-0.26; 1.59+/-0.32, green tea: 1.06+/-0.30; 1.54+/-0.55) significantly reduced the dentin wear when compared to control (2.00+/-0.55; 2.41+/-0.83) for both conditions. There were not significant differences among green tea extract, chlorhexidine and F solutions. CONCLUSIONS: Thus, the possible MMP-inhibitors tested in this study seem to be a promising preventive measure to reduce dentin erosion-abrasion, but their mechanism of action needs to be investigated in further studies. Chlorhexidine and green tea extract reduce dentin erosion and abrasion in situ. 2Chlorhexidine and green tea extract reduce dentin erosion and abrasion in situ ABSTRACT Objectives: This in situ/ex vivo study aimed to analyse the impact of possible MMP-inhibitors (chlorhexidine and green tea extract) on dentin wear induced by erosion or erosion plus abrasion. Methods:Twelve volunteers took part in this crossover and double-blind study performed in 4 phases of each 5 days. Bovine dentin samples were worn in palatal appliances and subjected to extraoral erosion (4 times/day, Coca-Cola, 5 min) or erosion plus abrasion (2 times/day, fluoridefree toothpaste and electrical toothbrush, 15s/sample). Immediately after each erosion, the appliances were reinserted in the mouth and the oral cavity was rinsed for 60 s with: 250 ppm F solution (SnF 2 /AmF, pH 4.5, Meridol-Gaba, Switzerland), 0.12% chlorhexidine digluconate (0.06% chlorhexidine, pH 6.0, Periogard-Colgate, Brazil), 0.61% green tea extract solution (OM24 ® , 100% Camellia Sinensis leaf extract, catechin concentration:30±3%, pH 7.0, Omnimedica, Switzerland) or deionized water (pH 6.0, control). Dentin loss was assessed by profilometry (µm). The data were analysed by two-way repeated m...

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“…Exposure to ultraviolet (UV) irradiation from the sun causes extrinsic aging, which can induce photoaging (Bae et al 2008); however, photoaging is mainly due to UVB irradiation exposureand is characterized by an altered pigmentation, inflammation and collagen degradation (Bennett 2008;Ahn et al 2013). Chronic exposure of skin to UVB irradiation also causes the production of matrix metalloproteinases (MMPs), resulting in the alteration of collagenous skin tissues (Ahn et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Potential prevention effects of Rubus occidentalis seed on UVB-induced MMP-1 production and procollagen degradation in CCD-986sk cells

Kim

Park

Son

2016

Journal of Applied Biological Chemistry

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UV exposure induces matrix metalloproteinases (MMPs) and extracellular matrix-degrading enzymes expression. We studied the protective effect of Rubus occidentalis seed against UVB-generated skin photoaging using human fibroblast cells (CCD-986sk). We used an ELISA kit to measure the supernatents of procollagen type I and MMP-1 in CCD-986sk cells after they were exposed to UVB irradiation. The CCD-986sk cells that were used with RC-E/E after the UVB irradiation caused higher levels of type I procollagen and lesser levels of MMP-1 compared with the control group. Furthermore, the RC-E/E treated group showed lesser MMP-1 levels and higher procollagen type I levels than the untreated counterpart. Therefore, it can be concluded that Rubus occidentalis seed can prevent from skin photoaging.

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(−)Epigallocatechin gallate hampers collagen destruction and collagenase activation in ultraviolet-B-irradiated human dermal fibroblasts: Involvement of mitogen-activated protein kinase

Cited by 137 publications

References 35 publications

The Protective Effects of Piceatannol from Passion Fruit (<i>Passiflora edulis</i>) Seeds in UVB-Irradiated Keratinocytes

The Protective Effects of Piceatannol from Passion Fruit (<i>Passiflora edulis</i>) Seeds in UVB-Irradiated Keratinocytes

Chlorhexidine and green tea extract reduce dentin erosion and abrasion in situ

Potential prevention effects of Rubus occidentalis seed on UVB-induced MMP-1 production and procollagen degradation in CCD-986sk cells

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