Hydrogen peroxide sensor HPCA1 is an LRR receptor kinase in Arabidopsis

Wu, Fei-Hua; Chi, Yuan; Jiang, Zhi; Xu, Yuanyuan; Xie, Ling; Huang, Feifei; Wan, Di; Ni, Jun; Yuan, Fang; Wu, Xiaomei; Zhang, Yanyan; Wang, Li; Rui, Yong; Byeon, Benjamin; Wang, Wenhua; Zhang, Shu; Sima, Matthew; Chen, Suping; Zhu, Minghua; Pei, Jessica; Johnson, Douglas M.; Zhu, Shan; Cao, Xiaoyang; Pei, Christopher; Zai, Zijing; Liu, Yihao; Liu, Tianyi; Swift, Gary B.; Zhang, Weiguo; Yu, Min; Hu, Zhangli; Siedow, James N.; Chen, Xian; Pei, Zhe

doi:10.1038/s41586-020-2032-3

Cited by 352 publications

(284 citation statements)

References 84 publications

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“…Interestingly, when salt-grown plants that had been sprayed with ascorbic acid were exposed to the darkness, they showed a delay in the stomatal closure. These findings are consistent with the role of H 2 O 2 as a modulator of stomata functioning [48].…”

Section: Redox-related Proteinssupporting

confidence: 90%

Sugar Beet (Beta vulgaris) Guard Cells Responses to Salinity Stress: A Proteomic Analysis

Rasouli

Kiani‐Pouya

et al. 2020

IJMS

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Soil salinity is a major environmental constraint affecting crop growth and threatening global food security. Plants adapt to salinity by optimizing the performance of stomata. Stomata are formed by two guard cells (GCs) that are morphologically and functionally distinct from the other leaf cells. These microscopic sphincters inserted into the wax-covered epidermis of the shoot balance CO2 intake for photosynthetic carbon gain and concomitant water loss. In order to better understand the molecular mechanisms underlying stomatal function under saline conditions, we used proteomics approach to study isolated GCs from the salt-tolerant sugar beet species. Of the 2088 proteins identified in sugar beet GCs, 82 were differentially regulated by salt treatment. According to bioinformatics analysis (GO enrichment analysis and protein classification), these proteins were involved in lipid metabolism, cell wall modification, ATP biosynthesis, and signaling. Among the significant differentially abundant proteins, several proteins classified as “stress proteins” were upregulated, including non-specific lipid transfer protein, chaperone proteins, heat shock proteins, inorganic pyrophosphatase 2, responsible for energized vacuole membrane for ion transportation. Moreover, several antioxidant enzymes (peroxide, superoxidase dismutase) were highly upregulated. Furthermore, cell wall proteins detected in GCs provided some evidence that GC walls were more flexible in response to salt stress. Proteins such as L-ascorbate oxidase that were constitutively high under both control and high salinity conditions may contribute to the ability of sugar beet GCs to adapt to salinity by mitigating salinity-induced oxidative stress.

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Section: Redox-related Proteinssupporting

confidence: 90%

Sugar Beet (Beta vulgaris) Guard Cells Responses to Salinity Stress: A Proteomic Analysis

Rasouli

Kiani‐Pouya

et al. 2020

IJMS

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“…ROS signalling widely exists in prokaryotic and eukaryotic organisms such as bacteria, yeast, animals and plants. Recently, an LRR receptor kinase HPCA1 was identified as H 2 O 2 in Arabidopsis [37]. However, no common regulatory pathway has been identified so far.…”

Section: Discussionmentioning

confidence: 99%

“…In Arabidopsis , hundreds of genes are annotated as “ROS signalling related” [34–36]. Recently, an LRR receptor kinase HPCA1 was identified as H 2 O 2 in Arabidopsis [37], however, very few ROS responsive DNA motifs were experimentally verified in plants [38–41]. Besides, the transcription factors that regulate the motif are yet to be identified.…”

Section: Introductionmentioning

confidence: 99%

The Identification of A ROS Responsive Motif that is regulated by snoRNP inArabidopsis

Cheng

Sun

Guo

2020

Preprint

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A GGGCC motif (site II like motif) was identified from the upstream sequences of reactive oxygen species (ROS) and light induced genes in Arabidopsis. This motif is highly enriched within the −50 to −250 bp region of the induced genes, and it is also specifically distributed in the same region in mouse and human genome. EMSA experiments revealed that several nuclear factors (NFs) bind to this motif, and the binding activities altered under H2O2 treatment. Two C/D family snoRNP proteins, namely fibrillarin 2 and NOP56, were identified from the site II like motif binding NFs. Several C/D family snoRNA, including R63, U24a and Z15, were also cloned from the motif binding NFs. These data suggest new regulatory roles of snoRNP in Arabidopsis.

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“…RSSR exchange between PRDX2 and STAT3 represses STAT3 transcriptional activity [53]. RSSR of plasma membrane bound HPLAC1 enables plants to sense and respond to extracellular H 2 O 2 [76].…”

Section: Disulfide Bonds (Rssr)mentioning

confidence: 99%

Immunological Techniques to Assess Protein Thiol Redox State: Opportunities, Challenges and Solutions

Cobley

Husi

2020

Antioxidants

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To understand oxidative stress, antioxidant defense, and redox signaling in health and disease it is essential to assess protein thiol redox state. Protein thiol redox state is seldom assessed immunologically because of the inability to distinguish reduced and reversibly oxidized thiols by Western blotting. An underappreciated opportunity exists to use Click PEGylation to realize the transformative power of simple, time and cost-efficient immunological techniques. Click PEGylation harnesses selective, bio-orthogonal Click chemistry to separate reduced and reversibly oxidized thiols by selectively ligating a low molecular weight polyethylene glycol moiety to the redox state of interest. The resultant ability to disambiguate reduced and reversibly oxidized species by Western blotting enables Click PEGylation to assess protein thiol redox state. In the present review, to enable investigators to effectively harness immunological techniques to assess protein thiol redox state we critique the chemistry, promise and challenges of Click PEGylation.

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Hydrogen peroxide sensor HPCA1 is an LRR receptor kinase in Arabidopsis

Cited by 352 publications

References 84 publications

Sugar Beet (Beta vulgaris) Guard Cells Responses to Salinity Stress: A Proteomic Analysis

Sugar Beet (Beta vulgaris) Guard Cells Responses to Salinity Stress: A Proteomic Analysis

The Identification of A ROS Responsive Motif that is regulated by snoRNP inArabidopsis

Immunological Techniques to Assess Protein Thiol Redox State: Opportunities, Challenges and Solutions

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