Immunoassays for inhibin and its subunits Further applications of the synthetic peptide approach

Age-dependent inhibin B concentration in relation to FSH and semen sample qualities: a study in 2448 men

“…During recent decades, the inhibin family was the hormone group considered to be relevant to reproduction and was therefore studied intensively and characterized (Meachem et al 2001). The development of specific immunoassays for the biologically active, dimeric glycoprotein form of inhibin (Groome & O'Brien 1993) proved that inhibin B is the only inhibin protein present in male serum , Hayes et al 2001. McCullagh (1932) postulated the existence of inhibin as a nonsteroidal substance from the testis capable of affecting pituitary function in 1932.…”

Second-trimester maternal serum inhibin-A screening for fetal Down syndrome in Asian women

“…This elevation is similar to that reported by Wald et al (1996), Cuckle et al (1996), Spencer et al (1996) and D' Antona et al (1998), but is lower than that reported by Aitken et al Wallace et al (1996), Wenstrom et al (1997a) and Haddow et al (1998) (Table 4). In all these series, inhibin-A was assayed using the method described by Groome and O'Brien (1993), but with different modifications and calibrator preparations, making it difficult to compare results between centres. This highlights the importance of the development of reliable and commercially available assays.…”

“…Serum inhibin-A assay was performed by a commercially available solid phase sandwich enzyme-linked immunosorbent assay (Serotec, Oxford, UK) (Groome and O'Brien, 1993) at the laboratory of the University of Hong Kong over four weeks, without knowing whether the sample was from a case or a control. The relation between inhibin-A and gestational age (weeks) in the controls was modelled using a quadratic regression equation on individual data, applied to a logarithmic transformation of inhibin-A, since inhibin-A was log-normally distributed.…”

Second-Trimester Dimeric Inhibin-a in Down's Syndrome Screening

“…The commercial assay will detect nonspecifically all forms of circulating inhibin, dimeric and free alpha subunits, whether fully or partially processed (Schneyer et al, 1990). More recently, a new specific assay for dimeric inhibin-A has been developed (Groome and O'Brien, 1993;Groome et al, 1994) and in studies of a first-trimester and two second-trimester (Cuckle et al, 1995;Wallace et al, 1996) series of Down's cases, levels of inhibin-A have been shown to be elevated. In order to assess the value of dimeric inhibin-A as a potential marker of Down's syndrome in the second trimester, we have studied this marker in a large series of affected and unaffected cases.…”