Lipidomic analysis of twenty‐seven prostanoids and isoprostanes by liquid chromatography/electrospray tandem mass spectrometry

Masoodi, Mojgan; Nicolaou, Anna

doi:10.1002/rcm.2697

Cited by 128 publications

(122 citation statements)

References 21 publications

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“…The sensitivity was almost equivalent to the detection limits reported in other articles: 0.3 ± 0.1 pg on column for PGE2 and PGD2, 28 10 pg on column for PGD2, 5 pg on column for PGF2α and TXB2, 50 pg on column for 6-keto PGF1α, 33 and 4 pg on column for LTB4. 34 To evaluate the validity of the calibration curve, spike-and-recovery tests were performed.…”

Section: Quantitative Validationsupporting

confidence: 77%

A Simple and Highly Sensitive Quantitation of Eicosanoids in Biological Samples Using Nano-flow Liquid Chromatography/ Mass Spectrometry

Ando

Satomi

2018

ANAL. SCI.

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A simple sample preparation method for eicosanoid was developed by the combination of deproteinization and nanoLC-ESI-MS/MS. Eicosanoids are a group of bioactive lipid mediators, present in trace amounts in the body. Therefore, an analytical method for eicosanoids requires superior sensitivity. The method described in this report, which takes advantage of the highly sensitive power of nanoLC-ESI-MS/MS, enabled a simplification of the sample-preparation process. Eicosanoid extraction was performed just by homogenization in methanol with subsequent phospholipid removal, and then the liquid phase was directly subjected to nanoLC-ESI-MS/MS analysis without a condensation process. The quantitation range achieved 0.01 -100 ng/mL for thromboxane B2, and 0.05 -100 ng/mL for prostaglandin E2, prostaglandin D2, prostaglandin F2, leukotriene B4, 6-keto prostaglandin F1α and 11-dehydro thromboxane B2. Rat brain sample analyses demonstrated the feasibility of the quantification of those seven eicosanoids from biological samples.

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Section: Quantitative Validationsupporting

confidence: 77%

A Simple and Highly Sensitive Quantitation of Eicosanoids in Biological Samples Using Nano-flow Liquid Chromatography/ Mass Spectrometry

Ando

Satomi

2018

ANAL. SCI.

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“…MS detection was performed with repetitive cycles of MRM transitions for important preselected metabolites followed by a full scan from m/z 100 to 1000. Catecholamines and eicosanoids were measured by offline and online SPE-LC-MS/MS (21)(22). Absolute quantification was performed by means of stable isotope-labeled standards.…”

Section: Metabolite Profiling Analysismentioning

confidence: 99%

Quality Markers Addressing Preanalytical Variations of Blood and Plasma Processing Identified by Broad and Targeted Metabolite Profiling

Kamlage¹,

Maldonado²,

Bethan³

et al. 2014

Clinical Chemistry

160

174

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BACKGROUND:Metabolomics is a valuable tool with applications in almost all life science areas. There is an increasing awareness of the essential need for high-quality biospecimens in studies applying omics technologies and biomarker research. Tools to detect effects of both blood and plasma processing are a key for assuring reproducible and credible results. We report on the response of the human plasma metabolome to common preanalytical variations in a comprehensive metabolomics analysis to reveal such high-quality markers.

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“…Extracts were subsequently purified by SPE (Masoodi and Nicolaou 2006) (Table 1). MassHunter software (Agilent Technologies Inc., CA, USA) was used for HPLC system control, data aquistion and data processing.…”

Section: Eicosanoid Analysismentioning

confidence: 99%

Stress and expression of cyclooxygenases (cox1, cox2a, cox2b) and intestinal eicosanoids, in Atlantic salmon, Salmo salar L.

Olsen

Svardal

Eide

et al. 2011

Fish Physiol Biochem

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Havforskningsinstituttets institusjonelle arkiv Brage IMR - Institutional repository of the Institute of Marine Research b r a g e i m rDette er forfatters siste versjon av den fagfellevurderte artikkelen, vanligvis omtalt som postprint. I Brage IMR er denne artikkelen ikke publisert med forlagets layout fordi forlaget ikke tillater dette. Du finner lenke til forlagets versjon i Brage-posten. Det anbefales at referanser til artikkelen hentes fra forlagets side. Ved lenking til artikkelen skal det lenkes til post i Brage IMR, ikke direkte til pdf-fil.This is the author's last version of the article after peer review and is not the publisher's version, usually referred to as postprint. You will find a link to the publisher's version in Brage IMR. It is recommended that you obtain the references from the publisher's site.Linking to the article should be to the Brage-record, not directly to the pdf-file. Foto: Leif NøttestadFish Physiology and Biochemistry Stress and expression of cyclooxygenases (cox1, cox2a, cox2b) and intestinal eicosanoids, in Atlantic salmon, Salmo salar.--Manuscript Draft-- Abstract: Prostaglandin H synthetases (cyclooxygensases) catalyze the initial reactions leading to prostanoids in animals. They form interesting links between diet and fish physiology as the type and nature of eicosanoids are affected by dietary lipid sources. Their expression is likely to be affected by tissues and also altered environmental conditions leading to altered amount and ratio of eicosanoids. These mechanisms are however poorly understood in fish. In the present study, Atlantic salmon Salmo salar (1000g, 10°C, seawater) were subjected to acute chasing stress. Liver, kidney, spleen, gill, muscle, midgut and hindgut were extracted before and 1h post stress and analyzed for mRNA expression of cox1, cox2a and cox2b. Intestinal samples were furthermore sampled over 24h for both cox expression and eicosanoid content. Results show a highly variable but consecutively expression of cox1, cox2a, cox2b in most tissues analyzed. Low levels were only found for cox2a in liver and cox2b in liver and kidney. The study reveals the general trend that cox1 is about 10 times the level of cox2b which again is about 10 times the level of cox2a. Cox2b shows the highest level of expression in the gills indicating a possible higher requirement for this protein in gills. Imposing stress to the fish induce a temporal increase in the expression of cox2a in the midgut while the gene expression of the other genes is not affected in any of the tissues analyzed. There is however a general tendency to increased expression of both cox2 genes that merits further studies. Stress had a profound effect on the intestinal eicosanoid content which showed a general decrease in midgut sections after stress that persisted for at least 24h. Response to Reviewers:We have checked the manuscript for spelling erros. For the notations of genes and proteins as suggested by the referee, we have doen the following, which is the current convention on labelling. We ...

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Lipidomic analysis of twenty‐seven prostanoids and isoprostanes by liquid chromatography/electrospray tandem mass spectrometry

Cited by 128 publications

References 21 publications

A Simple and Highly Sensitive Quantitation of Eicosanoids in Biological Samples Using Nano-flow Liquid Chromatography/ Mass Spectrometry

A Simple and Highly Sensitive Quantitation of Eicosanoids in Biological Samples Using Nano-flow Liquid Chromatography/ Mass Spectrometry

Quality Markers Addressing Preanalytical Variations of Blood and Plasma Processing Identified by Broad and Targeted Metabolite Profiling

Stress and expression of cyclooxygenases (cox1, cox2a, cox2b) and intestinal eicosanoids, in Atlantic salmon, Salmo salar L.

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