Lipoic acid inhibits leptin secretion and Sp1 activity in adipocytes

Prieto-Hontoria, Pedro L.; Pérez-Matute, Patricia; Fernández‐Galilea, Marta; Martínéz, J. Alfredo; Moreno-Aliaga, María J.

doi:10.1002/mnfr.201000534

Cited by 38 publications

(22 citation statements)

References 63 publications

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“…Previous studies have demonstrated the ability of LA to regulate the production of several adipokines involved in energy metabolism such as leptin [13,28,29].…”

Section: Discussionmentioning

confidence: 99%

Effects of lipoic acid on apelin in 3T3-L1 adipocytes and in high-fat fed rats

et al. 2011

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Lipoic acid (LA) is an antioxidant with therapeutic properties on several diseases like diabetes and obesity. Apelin is a novel adipokine with potential beneficial actions on glucose metabolism and insulin resistance. The aim of this study was to examine in 3T3-L1 adipocytes the effects of LA on apelin gene expression and secretion, as well as elucidate the signaling pathways involved. We also tested the regulation of adipose apelin gene expression by LA supplementation in a model of high-fat diet-induced obesity. LA increased apelin secretion but not apelin gene expression in 3T3-L1 adipocytes. The AMPK inhibitor Compound C induced an increase in LA-stimulated apelin production, and, on the contrary, the AMPK activator AICAR completely reversed the LA stimulatory effects on apelin secretion, also inducing a significant reduction in apelin mRNA levels in this in vitro model. Apelin mRNA levels were increased in those animals fed with the high-fat diet, while the caloric restriction decreased apelin mRNA to control levels. However, apelin gene expression was not significantly modified in rats treated with LA compared with the obese group. The current data suggest the ability of LA to modulate apelin secretion by adipocytes. However the insulin-sensitizing effect of LA in vivo is not related to changes in apelin gene expression in our model of diet-induced obesity.

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“…Previous studies have demonstrated the ability of LA to regulate the production of several adipokines involved in energy metabolism such as leptin [13,28,29].…”

Section: Discussionmentioning

confidence: 99%

Effects of lipoic acid on apelin in 3T3-L1 adipocytes and in high-fat fed rats

et al. 2011

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“…Parameters corresponding to OLIP group (co-treatment with LA) have been previously reported [36,42]. Values are mean ±SEM.…”

Section: Discussionmentioning

confidence: 99%

“…Metabolic parameters (body weight gain, WAT and liver weight, liver triglyceride content, plasma triglyceride and free fatty acids) were determined as previously described [33,34,42,43].…”

Section: Reagentsmentioning

confidence: 99%

Essential role of Nrf2 in the protective effect of lipoic acid against lipoapoptosis in hepatocytes

Valdecantos

Prieto-Hontoria

Pardo

et al. 2015

Free Radical Biology and Medicine

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“…Previous studies have demonstrated the ability of LA to modulate ERK, JNK, and Akt signaling pathways ( 8,9,29 ) as well as AMPK activity ( 30,31 ) in different cell types. Moreover, LA stimulates cAMP production in purifi ed human NK cells ( 32 ) and modulates the production of PGE 2 in osteoblasts ( 33 ).…”

Section: Analysis Of Mrna Levelsmentioning

confidence: 99%

Effects of lipoic acid on lipolysis in 3T3-L1 adipocytes

Fernández‐Galilea

Pérez-Matute

Prieto-Hontoria

et al. 2012

Journal of Lipid Research

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This article is available online at http://www.jlr.org properties. In fact, LA is able to directly scavenge reactive oxygen species (ROS) and regenerate endogenous antioxidants, such as glutathione and vitamins E and C ( 1, 2 ). Moreover, several studies have described potential beneficial effects of LA on obesity and its associated comorbidities, such as insulin resistance, type 2 diabetes, or fatty liver diseases. Thus, in rodents LA has been shown to cause profound weight loss by reducing food intake and enhancing energy expenditure ( 3 ) as well as by inducing a reduction on intestinal sugar absorption ( 4 ). More recently, two clinical trials in humans reported that LA caused signifi cant reductions of body weight, body mass index, blood pressure, and abdominal circumference in obese subjects ( 5, 6 ). LA also improved insulin sensitivity and plasma lipid profi le possibly through amelioration of oxidative stress and chronic infl ammatory status in obese patients with impaired glucose tolerance ( 7 ). Previous studies have provided strong evidence that LA is able to deeply affect adipose tissue development and function by the inhibition of adipogenesis ( 8 ), the regulation of the secretion of several adipokines such as leptin ( 9 ) and apelin ( 10 ), and by the promotion of mitochondrial biogenesis ( 11 ).In this context, previous studies suggested that LA seems to stimulate the lipolytic response in an in vitro model of broiler chicken adipocytes ( 12 ). However, the molecular mechanisms that mediate these effects remain unclear. Lipolysis is a complex process that is highly regulated and Lipoic acid (LA), or 1,2-dithiolane-3-pentaenoic acid, is a naturally occurring compound that contains two thiol groups with diverse benefi cial effects on health. The biological effects of LA were primarily associated with its antioxidant This work was supported, in part,

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Lipoic acid inhibits leptin secretion and Sp1 activity in adipocytes

Abstract: Abbreviations: FBS, foetal bovine serum; LA, lipoic acid; LASY, lipoic acid synthase; PF, pair-fed; PP1, protein phosphatase 1; SE, standard error; WAT, white adipose tissue.Keywords: Lipoic acid, Obesity, Leptin, Sp1 transcription factor, PI3K.

Cited by 38 publications

References 63 publications

Effects of lipoic acid on apelin in 3T3-L1 adipocytes and in high-fat fed rats

Effects of lipoic acid on apelin in 3T3-L1 adipocytes and in high-fat fed rats

Essential role of Nrf2 in the protective effect of lipoic acid against lipoapoptosis in hepatocytes

Effects of lipoic acid on lipolysis in 3T3-L1 adipocytes

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