Meat traceability using DNA markers: application to the beef industry

Arana, A.; Soret, B.; Lasa, Íñigo; Alfonso, L.

doi:10.1016/s0309-1740(01)00206-6

Cited by 59 publications

(42 citation statements)

References 3 publications

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“…Average heterozygocity (H) and average polymorphism information content (PIC) were calculated with the Molkin 3.0 software (Gutiérrez et al, 2005). Values of match probability (MP), defined as the probability of finding two individuals sharing by chance the same genotypic profile, were calculated according to Weir (1996) and Arana et al (2002). Match probability values were calculated using different marker sets, starting with the most polymorphic marker and adding sequentially the next most polymorphic.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, traceability is also extremely important for the protection and value enhancement of brands, especially in niche markets (Vetharaniam et al, 2009). For example, a label such as Ternera de Navarra (Beef of Navarra), a protected geographical indication (PGI) in the North of Spain, is certified by using DNA markers (Arana et al, 2002). The concept of traceability throughout the food supply chain is recognized within the European Union with the regulation (EC) No.…”

Section: Introductionmentioning

confidence: 99%

“…However, with these methods fraud cannot be completely prevented (Orru et al, 2006). On the contrary, DNA-based traceability systems or molecular traceability offer an unequivocal verification tool (Arana et al, 2002). Meat traceability studies differing in the number of microsatellites used, optimization of PCR conditions aiming at carrying out multiplex reactions and cattle breeds were recently reviewed (Rodríguez-Ramírez et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Meat traceability studies differing in the number of microsatellites used, optimization of PCR conditions aiming at carrying out multiplex reactions and cattle breeds were recently reviewed (Rodríguez-Ramírez et al, 2011). While in one study, eight microsatellites were used for the certification of Ternera de Navarra (Beef of Navarra) from the Pirenaica breed (Arana et al, 2002), other studies evaluated a larger number of microsatellites in several Italian cattle breeds (Orru et al, 2006;Dalvit et al, 2008a,b). In one study, 21 microsatellites were tested for breed identification in four native Italian cattle breeds (Dalvit et al, 2008a); the marker sets with the highest gene diversity were shown to perform best.…”

Section: Introductionmentioning

confidence: 99%

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Molecular traceability of beef from synthetic Mexican bovine breeds

Rodríguez-Ramírez¹,

Arana²,

Alfonso³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. Traceability ensures a link between carcass, quarters or cuts of beef and the individual animal or the group of animals from which they are derived. Meat traceability is an essential tool for successful identification and recall of contaminated products from the market during a food crisis. Meat traceability is also extremely important for protection and value enhancement of good-quality brands. Molecular meat traceability would allow verification of conventional methods used for beef tracing in synthetic Mexican bovine breeds. We evaluated a set of 11 microsatellites for their ability to identify animals belonging to these synthetic breeds, Brangus and Charolais/ Brahman (78 animals). Seven microsatellite markers allowed sample discrimination with a match probability, defined as the probability of finding two individuals sharing by chance the same genotypic profile, of 10 -8 . The practical application of the marker set was evaluated by testing eight samples from carcasses and pieces of meat at the slaughterhouse and at the point of sale. The DNA profiles of the two samples obtained at these two different points in the productioncommercialization chain always proved that they came from the same animal.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular traceability of beef from synthetic Mexican bovine breeds

Rodríguez-Ramírez¹,

Arana²,

Alfonso³

et al. 2011

Genet. Mol. Res.

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“…Traceability of beef requires a verifiable method to identify bovine animals, carcasses and cuts in all their packaging and transport/storage configurations at any point in the supply chain. Unique identification numbers must be applied and recorded accurately to guarantee a link between each point of processing in the supply chain (Cunningham & Meghen 2001;Arana et al 2002). The system structure of complete traceability from farm to consumer is shown in Fig.…”

Section: Radio Frequency Identification In Beef Trackingmentioning

confidence: 99%

A traceability system for beef products based on radio frequency identification technology in China

Sun

Yang

et al. 2007

New Zealand Journal of Agricultural Research

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Radio frequency identification (RFID) technology is an automatic identification and data capture (AIDC) technique and it is the representative technology for handling beef traceability. In this paper we offer a complete solution including: frequency, identifier information system, information system, data organisation, tag reclaimation and control technology, from three segments of the beef production process in China. From the farm to the slaughterhouse, electronic identification ear tag technology was used to identify the individual animal. From the slaughterhouse to the processing plant, gambrel identification was used to transfer the carcass information from the "old ear tag" to a "new ear tag". Last, gambrel RFID was converted to UCC/EAN-128 barcode labelling with a wireless electronic scale. The whole solution was evaluated successfully in Beijing.

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