Molecular detection of &lt;em&gt;vanA&lt;/em&gt; and &lt;em&gt;vanB&lt;/em&gt; genes among vancomycin-resistant enterococci in ICU-hospitalized patients in Ahvaz in southwest of Iran

Moosavian, Mojtaba; Ghadri, H; Samli, Zahra

doi:10.2147/idr.s177886

Cited by 23 publications

(22 citation statements)

References 21 publications

(24 reference statements)

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“…[ 16 ], Moosavian et al. [ 19 ] and Gonzalez et al. [ 20 ], Enterococcus species had a high frequency in patients' rectal samples.…”

Section: Discussionmentioning

confidence: 99%

“…applied samples from the wound, blood, cerebrospinal fluid and pleural fluid [ 1 ]. Moreover, exposure to dissimilar antibiotics, duration of antibiotic therapy, different detection methods and different sample sizes could possibly affect the prevalence rate of Enterococcus species [ 1 , 19 ]. In the current study, 95.1% of patients were under antibiotic therapy and most patients had used different antibiotics such as cephalosporins, aminoglycoside, meropenem and vancomycin for 1–5 days.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Frequency and co-colonization of vancomycin-resistant Enterococci and Candida in ICU-hospitalized children

Shirvani

Behzad

Abdollahi

et al. 2021

New Microbes and New Infections

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In the time span between January 2018 and September 2020, 205 patients were enrolled in a prospective cohort study at Mofid Children's Hospital. Demographic information and clinical data on all the participating children were collected and rectal swabs were performed for the sampling method. All samples were analysed so as to identify the presence of Enterococcus and Candida colonization by the use of conventional biochemical tests. Resistance to vancomycin in Enterococcus isolates was phenotypically identified using an E-test kit and MIC value, interpreted according to the CLSI criteria. The presence of vanA and vanB genes, which encode the resistance to vancomycin, was screened by PCR assay. Candida species were detected in 21.5% of rectal swab samples. Candida glabrata (56.8%) and Candida albicans (43.2%) were the only Candida species detected. Enterococcus species were detected in 29.3% of rectal swab samples. Out of 60 Enterococcus isolates, 33 (55%) were resistant to vancomycin. Moreover, vanA was detected in 84.8% and vanB was detected in 3% of the 33 vancomycin-resistant Enterococcus isolates. Enterococcus and Candida species were frequently detected in the <1 year and 1–3 years age groups, respectively. Central venous access catheter and brain tumour were the main reasons for hospital admissions, 32.2% and 20.1% of total admissions, respectively. Furthermore, it must be noted that the most frequent underlying medical conditions in participating patients were esophageal atresia and hydrocephalus. The results of the present study demonstrated the necessity of determining the susceptibility of Enterococcus isolates to vancomycin before prescribing antibiotics.

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“…[ 16 ], Moosavian et al. [ 19 ] and Gonzalez et al. [ 20 ], Enterococcus species had a high frequency in patients' rectal samples.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Frequency and co-colonization of vancomycin-resistant Enterococci and Candida in ICU-hospitalized children

Shirvani

Behzad

Abdollahi

et al. 2021

New Microbes and New Infections

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“…6,7 Linezolid resistance in Enterococcus species is related to a mutation in the 23S rRNA gene region as well as the presence of the plasmid-encoded chloramphenicol-florfenicol resistance (cfr) gene. 6,27,28 According to various studies, the rate of linezolid resistance in enterococci is low in Iran (0.7%), Turkey (1%), and Australia (1.3%) 17,23,29 but relatively high in Canada (13.7%). 30 In this study, all VREfm strains were susceptible to daptomycin.…”

Section: Dovepressmentioning

confidence: 99%

<p>Antibiotic Resistance and Molecular Epidemiology of Vancomycin-Resistant Enterococci in a Tertiary Care Hospital in Turkey</p>

Aşgın

Otlu

2020

IDR

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Purpose: Vancomycin-resistant enterococci (VRE) have become a global health threat in the last two decades. In this study, we aimed to determine antibiotic resistance using phenotypic and genotypic methods in VRE strains obtained from inpatients and to investigate clonal relatedness among strains. Methods: Identification and antibiotic susceptibility of 47 VRE strains obtained from inpatients at Karabuk University Hospital from 2014 to 2015 were determined using the BD Phoenix™ automated microbiology system. Vancomycin resistance genes (Van A and B) were detected by polymerase chain reaction. Clonal relatedness among the strains was evaluated by pulsed-field gel electrophoresis (PFGE). Results: All 47 VRE strains obtained from rectal (n=35), blood (n=7), and urine (n=5) samples were confirmed as Enterococcus faecium; they were resistant to ampicillin, gentamicin, vancomycin, and teicoplanin. One E. faecium isolate was intermediately resistant to linezolid. No strain was resistant to quinupristin-dalfopristin or daptomycin. Only vanA was detected among strains. According to the PFGE results, 31 of 47 strains were clonally related with a clustering rate of 66%. No common clone was detected. Conclusion: VRE infections are associated with high mortality, morbidity, and healthcare expenditures. Increasing resistance to last-line drugs, such as linezolid and daptomycin, among VRE strains is a great concern. Therefore, comprehensive measures should be performed to reduce VRE colonization. Although there was no common clone VRE outbreak, polyclonal spread was observed in our hospital. The high clustering rate indicated cross-contamination. Thus, a more effective infection control program should be implemented.

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“…Moreover, acquired resistance through lateral gene transfer to other available antimicrobial agents, including: beta-lactams, aminoglycosides (high level resistance), macrolides, glycopeptides, and oxazolidinones, makes it more challenging 10. Vancomycin resistant enterococci (VRE) have been reported as a leading cause of outbreaks of hospital-acquired infections and in ICU-hospitalized patients 11,12. Linezolid was the drug of choice for management of infections caused by VRE; however, linezolid resistant enterococci have emerged and are reported 13,14…”

Section: Introductionmentioning

confidence: 99%

<p>Emergence of multidrug resistance and extensive drug resistance among enterococcal clinical isolates in Egypt</p>

Said¹,

Abdelmegeed²

2019

IDR

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Introduction Enterococci commonly inhabit the gastrointestinal tract of both human and animals; however, they have emerged as a leading cause of several infections with substantial morbidity and mortality. Their ability to acquire resistance combined with intrinsic resistance to various antimicrobials makes treatment of enterococcal infections challenging. Materials and methods The aim of the study was to evaluate the antimicrobial resistance pattern, and assess the prevalence of multidrug resistance (MDR) and extensive drug resistance (XDR) among enterococcal isolates, collected from different clinical sources, in Mansoura University Hospitals, Egypt. Results Antibiotic sensitivity testing revealed elevated levels of resistance among enterococcal clinical isolates (N=103). All E. faecium (N=32) and 74.6% of E. faecalis isolates (N=71) were MDR, while two E. faecalis and four E. faecium isolates were XDR. High level gentamicin resistance was detected in 79.6%, most of them carried the aac(6’)-Ie-aph(2’’)-Ia gene. High level streptomycin resistance was seen in 36.9%, of which 52.6% carried the ant(6’)-Ia gene. Resistance to macrolides and lincosamides were mediated by ermB (92.2%) and msrA/B (42.7%). tetK , tetL , and tetM genes were detected among tetracyclines resistant isolates. Resistance to vancomycin was detected in 15.5%, where vanB and vanC 1 gene clusters were detected in VRE isolates. Ten isolates (9.7%) were resistant to linezolid, eight of which harbored the optrA gene. Vancomycin and linezolid resistant enterococci were more likely to exhibit strong/moderate biofilm formation than vancomycin and linezolid sensitive ones. Conclusion Elevated levels of resistance to different classes of antimicrobial agents and emergence of MDR and XDR strains pose a major threat with limited therapeutic options for infections caused by this emerging pathogen.

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Molecular detection of <em>vanA</em> and <em>vanB</em> genes among vancomycin-resistant enterococci in ICU-hospitalized patients in Ahvaz in southwest of Iran

Cited by 23 publications

References 21 publications

Frequency and co-colonization of vancomycin-resistant Enterococci and Candida in ICU-hospitalized children

Frequency and co-colonization of vancomycin-resistant Enterococci and Candida in ICU-hospitalized children

<p>Antibiotic Resistance and Molecular Epidemiology of Vancomycin-Resistant Enterococci in a Tertiary Care Hospital in Turkey</p>

<p>Emergence of multidrug resistance and extensive drug resistance among enterococcal clinical isolates in Egypt</p>

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