On the karyotypic origin and evolution of cancer cells

Nicholson, Joshua M.; Duesberg, Peter H.

doi:10.1016/j.cancergencyto.2009.06.008

Cited by 56 publications

(55 citation statements)

References 59 publications

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“…and pipetted with plastic pipette tips onto microscope slides following published procedures. 50,51 Slides with suitable metaphase chromosomes were then hybridized with chromosomespecific, color-coded DNA probes as described by the manufacturer (MetaSystems) and by us previously in references 50 and 51.…”

Section: Methodsmentioning

confidence: 99%

“…Since cancers are species with flexible karyotypes, they can generate new subspecies, such as metastases, by spontaneous karyotypic rearrangements, independent of mutation. 17,[47][48][49][50][51][52] This flexibility of cancer karyotypes is a consequence of an equilibrium between two competing forces: (1) inherited imbalances of normal mitosis genes destabilizing cancer karyotypes. These imbalances reflect the very karyotypic rearrangements that generated cancers from normal cells.…”

Section: Origin Of Metastasesmentioning

confidence: 99%

“…To test the karyotypic relationships between A13A, A13B and A13D, we have compared their karyographs. 17,50 Karyographs are three-dimensional tables that list the numbers of intact chromosomes and number designations of marker chromosomes on the x-axis, the cancer-specific copy numbers of each chromosome on the y-axis, and the number of about 20 metaphases arrayed per table on the z-axis. By comparing 20 karyograms at once, karyographs immediately distinguish clonal from non-clonal chromosomes.…”

Section: Origin Of Metastasesmentioning

confidence: 99%

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Origin of metastases: Subspecies of cancers generated by intrinsic karyotypic variations

et al. 2012

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Section: Methodsmentioning

confidence: 99%

Section: Origin Of Metastasesmentioning

confidence: 99%

Section: Origin Of Metastasesmentioning

confidence: 99%

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Origin of metastases: Subspecies of cancers generated by intrinsic karyotypic variations

et al. 2012

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“…Except four abnormal chromosomes [del(2), del(11), t(15;19) and t(3;20)] are found in common, the other ten derived chromosomes: A1[del (1q) (12)] appeared in present study are not found in previous A549 karyotype. As shown previously, many cancer cells could quickly change their karyotypes during the process of culturing in vitro (Dano et al, 2001;Nicholson & Duesberg, 2009). The differences between A549 karyotypes previously published and presented here may be due to different passages of subculturing.…”

Section: Comparison With Previously Published A549mentioning

confidence: 64%

Characterization of Two Human Lung Adenocarcinoma Cell Lines by Reciprocal Chromosome Painting

Peng¹,

Wang²,

Su³

et al. 2010

Zoological Research

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Lung cancer is a leading cause of cancer death worldwide. Some lung cancer patients correlate with a gas of radon besides smoking. To search for common chromosomal aberrations in lung cancer cell lines established from patients induced by different factors, a combined approach of chromosome sorting, forward and reverse chromosome painting was used to characterize karyotypes of two lung adenocarcinoma cell lines: A549 and GLC-82 with the latter line derived from a patient who has suffered long-term exposure to environmental radon gas pollution. The chromosome painting results revealed that complex chromosomal rearrangements occurred in these two lung adenocarcinoma cell lines. Thirteen and twenty-four abnormal chromosomes were identified in A549 and GLC-82 cell lines, respectively. Almost half of abnormal chromosomes in these two cell lines were formed by non-reciprocal translocations, the others were derived from deletions and duplication/or amplification in some chromosomal regions. Furthermore, two apparently common breakpoints, HSA8q24 and 12q14 were found in these two lung cancer cell lines.

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“…It has been suggested that adverse outcome associated with CIN may be related to high tumor cell heterogeneity driving the ability of tumors to adapt to environmental stress, including drug treatment (Chandhok and Pellman 2009;Nicholson and Duesberg 2009). Here we found different degrees of sensitivity to chemotherapeutic drugs between MB cell lines.…”

Section: Discussionmentioning

confidence: 99%

Chromosomal heterogeneity and instability characterize pediatric medulloblastoma cell lines and affect neoplastic phenotype

et al. 2013

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Chromosomal heterogeneity is a hallmark of most tumors and it can drive critical events as growth advantages, survival advantages, progression and karyotypic evolution. Medulloblastoma (MB) is the most common malignant central nervous system tumor in children. This work attempted to investigate chromosomal heterogeneity and instability profiles of two MB pediatric cell lines and their relationship with cell phenotype. We performed GTG-banding and cytokinesis-block micronucleus cytome assays, as well as morphological characterization, cell population doubling time, colony-forming efficiency, and chemo-sensitivity assays in two pediatric MB cell lines (UW402 and UW473). Both MB cells showed a high chromosomal heterogeneity. UW473 cells showed *2 fold higher both clonal-and non-clonal chromosomal alterations than UW402 cells. Besides, UW473 showed two clonal-groups well-differentiated by ploidy level (\2n[and\4n[) and also presented a significantly higher number of chromosomal instability biomarkers. These results were associated with high morphological heterogeneity and survival advantages for UW473 and proliferation advantages for UW402 cells. Moreover, UW473 was significantly more sensitive to methotrexate, temozolomide and cisplatin while UW402 cells were more sensitive to doxorubicin. These data suggest that distinct different degrees of karyotypic heterogeneity and instability may affect neoplasic phenotype of MB cells. These findings bring new insights into cell and tumor biology.

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On the karyotypic origin and evolution of cancer cells

Cited by 56 publications

References 59 publications

Origin of metastases: Subspecies of cancers generated by intrinsic karyotypic variations

Origin of metastases: Subspecies of cancers generated by intrinsic karyotypic variations

Characterization of Two Human Lung Adenocarcinoma Cell Lines by Reciprocal Chromosome Painting

Chromosomal heterogeneity and instability characterize pediatric medulloblastoma cell lines and affect neoplastic phenotype

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