OpenComet: An automated tool for comet assay image analysis

Gyori, Benjamin M.; Venkatachalam, Gireedhar; Thiagarajan, P. S.; Hsu, David; Clément, Marie‐Véronique

doi:10.1016/j.redox.2013.12.020

Cited by 532 publications

(370 citation statements)

References 28 publications

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“…An increase of the fluorescence intensity in the tail of the comet means that the number of breaks is increased and so more damage exist (Gyori et al, 2014). …”

Section: Methodsologies Available To Screen Anticancer Activitymentioning

confidence: 99%

Anticancer effects of seaweed compounds fucoxanthin and phloroglucinol, alone and in combination with 5-fluorouracil in colon cells

Lopes-Costa

Abreu

Gargiulo

et al. 2017

Journal of Toxicology and Environmental Health, Part A

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“…An increase of the fluorescence intensity in the tail of the comet means that the number of breaks is increased and so more damage exist (Gyori et al, 2014). …”

Section: Methodsologies Available To Screen Anticancer Activitymentioning

confidence: 99%

Anticancer effects of seaweed compounds fucoxanthin and phloroglucinol, alone and in combination with 5-fluorouracil in colon cells

Lopes-Costa

Abreu

Gargiulo

et al. 2017

Journal of Toxicology and Environmental Health, Part A

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“…The slides were then washed by prechilled distilled water, fixed by 70% ethanol, air-dried, and stained with Vista Green DNA dye. Images were acquired by a Nikon Ti-S microscope using a FITC filter, and the percentage of tail DNA of individual cells was determined by the OpenComet plugin of ImageJ [32]. The graph and twotailed Student's t-test were performed using the GraphPad Prism 7 software.…”

Section: Comet Assaymentioning

confidence: 99%

Canonical microRNAs Enable Differentiation, Protect Against DNA Damage, and Promote Cholesterol Biosynthesis in Neural Stem Cells

Liu

Zhang

Khodadadi‐Jamayran

et al. 2017

Stem Cells and Development

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Neural stem cells (NSCs) have the capacity to differentiate into neurons, astrocytes, and oligodendrocytes, and therefore represent a promising donor tissue source for treating neurodegenerative diseases and repairing injuries of the nervous system. However, it remains unclear how canonical microRNAs (miRNAs), the subset of miRNAs requiring the Drosha-Dgcr8 microprocessor and the type III RNase Dicer for biogenesis, regulate NSCs. In this study, we established and characterized Dgcr8 -/-NSCs from conditionally Dgcr8-disrupted mouse embryonic brain. RNA-seq analysis demonstrated that disruption of Dgcr8 in NSCs causes a complete loss of canonical miRNAs and an accumulation of pri-miRNAs. Dgcr8-/-NSCs can be stably propagated in vitro, but progress through the cell cycle at reduced rates. When induced for differentiation, Dgcr8-/-NSCs failed to differentiate into neurons, astrocytes, or oligodendrocytes under permissive conditions. Compared to Dgcr8 +/-NSCs, Dgcr8 -/-NSCs exhibit significantly increased DNA damage. Comparative RNA-seq analysis and gene set enrichment analysis (GSEA) revealed that Dgcr8 -/-NSCs significantly downregulate genes associated with neuronal differentiation, cell cycle progression, DNA replication, protein translation, and DNA damage repair. Furthermore, we discovered that Dgcr8 -/-NSCs significantly downregulate genes responsible for cholesterol biosynthesis and demonstrated that Dgcr8 -/-NSCs contain lower levels of cholesterol. Together, our data demonstrate that canonical miRNAs play essential roles in enabling lineage specification, protecting DNA against damage, and promoting cholesterol biosynthesis in NSCs.

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“…Para cada individuo se analizaron 100 células por placa (total dos placas). Las imágenes de los cometas fueron fotografiadas y analizadas con el programa Open Comet (14).…”

Section: Resultsunclassified

“…For each individual, 100 cells per plate (total two plates) were analyzed. The images of the comets were photographed and analyzed with the Open Comet program (14).…”

Section: Methodsmentioning

confidence: 99%

Determinación mediante prueba cometa del daño genético causado por uso de ivermectina en vacas cebú (Bos taurus indicus)

2017

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Objective. The objective of the work was evaluate the damage genetic caused by the use of ivermectin (IVM) in cows zebu to concentrations of 1% and 3.15% through the test comet. Material and methods. 15 cows, were taken with age between 3 and 4 years old, average weight of 350 kg, body condition between 3 and 3.5. Three experimental groups with five animals per group, which were exposed to the concentration of IVM to 1% to 3.15% more group control (without application of IVM) were used. Animal blood sample was performed by venipuncture jugular or medial flow with vacutainer ® needle, extracting 8 ml of blood. The blood samples it was collected at 9, 18 and 27 days post-treatment. Results. The display of the comets is made by using fluorescence microscope, the cells were evaluated by means of visual log and the Comet image software. Evidenced the presence of nuclei with DNA migration in all analyzed plates. The values of classification of comets indicate cells with high levels of damage (grade 3: cells with high damage). The rate of DNA damage of the treatment to 1% to 3.15% was significant, to relate to the control group. Conclusions. The results obtained in this study demonstrate the likely genotoxic potential of the use of IVM in cattle.Keywords: Genetic damage, ADN, mammals, drug (Source: CAB). RESUMENObjetivo. El objetivo del trabajo fue evaluar el daño genético causado por el uso de Ivermectina (IVM) en vacas cebú (Bos taurus indicus). Materiales y Métodos. Se tomaron quince vacas, con edad entre tres y cuatro años, peso promedio de 350 kilogramos, condición corporal entre 3 y 3,5. Se utilizaron tres grupos experimentales con cinco animales por grupo, los cuales se expusieron a la concentración de IVM al 1% y 3,15% más un grupo control (sin aplicación de IVM). La muestra sanguínea por animal fue realizada mediante punción venosa yugular o caudal medial con aguja para vacutainer ®, extrayéndose 8 ml de sangre. Las muestras sanguíneas fueron recolectadas a los 9, 18 y 27 días postratamiento. Resultados. La visualización de los cometas se realizaron con microscopio de fluorescencia, las células fueron evaluadas por medio de registro visual y por el software Comet INTRODUCCIÓNEl uso de productos de origen químicos, para mantener el ganado vacuno sano y con altos rendimientos productivos, se basa en la necesidad de satisfacer la creciente demanda mundial de productos de origen animal. Los fármacos se aplican en forma habitual y con fines profilácticos a los animales de producción, para el tratamiento de enfermedades parasitarias. Se tiene poco conocimiento de estudios realizados sobre la genotoxicidad (definida como daño en el ADN) y/o citotoxicidad de la IVM en individuos (vectores, mamíferos y humanos), para los cuales el compuesto es disponibilizado para el control parasitario (1,2). Sin embargo, existe amplia literatura in vivo asociada a la evaluación de las formas de administración, dosis terapéuticas (3), sensibilidad de invertebrados a la droga (4) y resistencias (5,6).Para detectar el daño geno...

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OpenComet: An automated tool for comet assay image analysis

Cited by 532 publications

References 28 publications

Anticancer effects of seaweed compounds fucoxanthin and phloroglucinol, alone and in combination with 5-fluorouracil in colon cells

Anticancer effects of seaweed compounds fucoxanthin and phloroglucinol, alone and in combination with 5-fluorouracil in colon cells

Canonical microRNAs Enable Differentiation, Protect Against DNA Damage, and Promote Cholesterol Biosynthesis in Neural Stem Cells

Determinación mediante prueba cometa del daño genético causado por uso de ivermectina en vacas cebú (Bos taurus indicus)

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