Real-Time PCR studies regarding the borrelia burgdorferi, francisella tularensis, tick borne encephalitis virus (TBEv) and crimeean congo hemorrhagic fever virus (CCHFv) occurrence in the Romanian ticks

Vladimirescu, A.; Dumitrescu, G.; Ionescu, L; Necşulescu, Marius; Moraru, Valentina; Popescu, D.; Bicheru, Simona; Daneş, Doina; Baraitareanu, D.; Ciulacu-Purcarea, V.; Nicolescu, Gabriela

doi:10.1016/j.ijid.2016.02.448

Cited by 6 publications

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“…platys Ab)(Table 4). Despite several Romanian reports concerning the circulation of Ehrlichia canis and B. burgdorferi in dogs(Kiss et al, 2011;Mircean et al, 2012;Morar et al, 2015;Enache & Coprean, 2015) and in tickvectors(Vladimirescu et al, 2016), in our study non Ehrlichia and Borrelia species were detected in…”

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confidence: 92%

Vector-Borne Pathogens-Testing in a Romanian Canine Blood Bank

Ionescu

Rădulescu

Florea

et al. 2018

CBUP

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INTRODUCTION: Canine blood banking in veterinary medicine is an expanding market. Once the demand for blood products increased all over the world, canine blood banks have focused attention on the risk of spreading diseases through blood transfused products. The need to preserve a healthy donor-pool, free of blood-borne infectious diseases, mainly in endemic areas, led to the implementation of appropriate protocols for screening canine blood donors using specific tests. OBJECTIVES: The aim of this study was to evaluate the presence of Anaplasma phagocytophilum/Anaplasmaplatys, Echrlichiacanis/Echrlichiaewingii, Dirofilariaimmitis and Borrelia burgdorferi using the enzyme immunoassay technology (EIA) among the donors of a Romanian canine blood bank, from January 2015 to December 2016. METHODS: Blood samples from 575 donors were collected and 1253 tests were performed with SNAP 4DX Plus® (IDEXX Laboratories, Fremont, CA) to reveal the presence of D. immitis antigens and the antibodies toward A. phagocytophilum and/or A. platys, E. canis and/or E. ewingii and B. burgdorferi. RESULTS: The results of this holistic approach show that all blood samples provided negative results for B. burgdorferi and E.canis/E. ewingii (0/1253), while 0.87% (11/1253) samples provided positive results for A. phagocytophilum/A. platys and 6.94% (87/1253) for D. immitis. CONCLUSION: The next studied topic would be to compare the results provided by the EIA technology with results of real time PCR and qPCR regarding these vector-borne pathogens. UDC Classification: 616.9; DOI: http://dx.

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confidence: 92%

Vector-Borne Pathogens-Testing in a Romanian Canine Blood Bank

Ionescu

Rădulescu

Florea

et al. 2018

CBUP

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“…All 14 cases originated in 5 counties in northwestern Romania; incidence was low (<0.5/100,000 inhabitants) in Bihor, Bistrița-Năsăud, Cluj, and Mureș and moderate (0.5–4.0/100,000 inhabitants) in Sălaj. In a recent survey done by collecting I. ricinus ticks from vegetation, livestock, and reptiles in central and southeastern Romania, the prevalence the TBE virus estimated by using molecular methods was <1% ( 13 ). Coipan et al evaluated the seropositivity for TBE in 51 patients who had neurologic signs and were admitted to a hospital in Sibiu County, Romania; specific TBE antibodies were found in samples from 38 (75%) patients ( 14 ).…”

Section: Discussionmentioning

confidence: 99%

Tick-Borne Encephalitis in Sheep, Romania

Salát¹,

Mihalca²,

Mihaiu³

et al. 2017

Emerg. Infect. Dis.

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TBE in Romania

Chiţimia-Dobler¹,

Hristea²,

Erber³

et al. 2021

Tick-Borne Encephalitis - The Book

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Based on an epidemiological survey,1 human TBEV neuroinfections may have an endemic emergent course, and natural foci are in full territorial expansion. Identified risk areas are Tulcea district, Transylvania, at the base of the Carpathian Mountains and the Transylvanian Alps.2,3 TBE has been a notifiable disease since 1996. Surveillance of TBE is not done at the country level, only regionally in some counties (northern/central/western part, close to Hungary). The passive surveillance system was implemented in 2008. However, there is no regular screening and the relative risk of contracting this disease is unknown. In 1999, an outbreak of TBE in humans was recorded with a total of at least 38 human cases.4 The probable cause of the outbreak was goat milk and raw goat milk products. Subsequent studies to detect TBEV in ticks in the affected regions resulted in a non -specified number of TBEV isolates, which were described as belonging to the European subtype of TBEV. A publication of the neighboring Republic of Moldova described the existence of the Far-eastern subtype of TBEV just at the border to Romania.5

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Real-Time PCR studies regarding the borrelia burgdorferi, francisella tularensis, tick borne encephalitis virus (TBEv) and crimeean congo hemorrhagic fever virus (CCHFv) occurrence in the Romanian ticks

Cited by 6 publications

References 0 publications

Vector-Borne Pathogens-Testing in a Romanian Canine Blood Bank

Vector-Borne Pathogens-Testing in a Romanian Canine Blood Bank

Tick-Borne Encephalitis in Sheep, Romania

TBE in Romania

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