Regulation of differentiation of sheep subcutaneous and abdominal preadipocytes in culture

Soret, B.; Hj, Lee; Finley, Eric; Sc, Lee; Vernon, R G

doi:10.1677/joe.0.1610517

Cited by 55 publications

(65 citation statements)

References 39 publications

(48 reference statements)

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“…This is consistent with there being depot-specific responses of preadipocytes to exogenous factors that regulate preadipocyte differentiation (Soret et al 1999). In our study, PR adipose tissue was susceptible to the programming effects of maternal nutrient intake during the first and second trimesters of gestation, whereas OM adipose tissue was only susceptible during the second trimester.…”

Section: Fetal Programming Of Bovine Adipose Tissuesupporting

confidence: 91%

Heifer nutrient intake during early- and mid-gestation programs adult offspring adiposity and mRNA expression of growth-related genes in adipose depots

Micke

Sullivan²,

McMillen³

et al. 2011

Reproduction

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Changes in maternal nutrient intake during gestation alter IGF receptor abundance and leptin (LEP) mRNA expression in fetal adipose tissue. It is not known whether such changes persist into adult life and whether they are associated with an effect on phenotype. We investigated the effect of high (240%) and low (70%) levels of recommended daily crude protein intake for beef heifers during the first and second trimesters of gestation on singleton progeny (nZ68): subcutaneous (SC) adipose tissue depth at rump (P8) and rib (RF) sites from 65 until 657 days of age; plasma leptin concentrations from birth until 657 days and expression of IGF1 and IGF2, their receptors (IGF1R and IGF2R) and LEP mRNA in perirenal (PR), omental (OM) and SC adipose tissue at 680 days of age. High-protein diets during the first trimester increased LEP and IGF1 mRNA in PR of males and females, respectively, compared with low-protein diets, and decreased IGF1R mRNA in SC of all progeny but increased RF depth of males between 552 and 657 days. High-protein diets compared with low-protein diets during the second trimester increased IGF1R mRNA in PR and OM of all progeny; LEP mRNA in PR of males; and IGF2 and IGF2R mRNA in OM of all progeny. Conversely, LEP mRNA in OM and IGF2 mRNA in PR of all progeny were decreased following exposure to high-compared with low-protein diets during the second trimester. Heifer diet during gestation has permanent sex-and depot-specific effects on the expression of adipogenic and adipocytokine genes and offspring adiposity.

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Section: Fetal Programming Of Bovine Adipose Tissuesupporting

confidence: 91%

Heifer nutrient intake during early- and mid-gestation programs adult offspring adiposity and mRNA expression of growth-related genes in adipose depots

Micke

Sullivan²,

McMillen³

et al. 2011

Reproduction

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“…This is supported by the central role of PPARg in the control of the terminal differentiation of pre-adipocytes isolated from the stromalvascular fraction in bovine WAT Torii et al, 1998) and ovine BAT (Soret et al, 1999). However, CEBP-a expression was either not detected or did not parallel the adipogenic process (Ohsaki et al, 2007) in cattle.…”

Section: Dynamics Of At Growthmentioning

confidence: 89%

Ontogenesis of muscle and adipose tissues and their interactions in ruminants and other species

Bonnet

Cassar-Malek

Chilliard

et al. 2010

Animal

105

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The lean-to-fat ratio, that is, the relative masses of muscle and adipose tissue, is a criterion for the yield and quality of bovine carcasses and meat. This review describes the interactions between muscle and adipose tissue (AT) that may regulate the dynamic balance between the number and size of muscle v. adipose cells. Muscle and adipose tissue in cattle grow by an increase in the number of cells (hyperplasia), mainly during foetal life. The total number of muscle fibres is set by the end of the second trimester of gestation. By contrast, the number of adipocytes is never set. Number of adipocytes increases mainly before birth until 1 year of age, depending on the anatomical location of the adipose tissue. Hyperplasia concerns brown pre-adipocytes during foetal life and white pre-adipocytes from a few weeks after birth. A decrease in the number of secondary myofibres and an increase in adiposity in lambs born from mothers severely underfed during early pregnancy suggest a balance in the commitment of a common progenitor into the myogenic or adipogenic lineages, or a reciprocal regulation of the commitment of two distinct progenitors. The developmental origin of white adipocytes is a subject of debate. Molecular and histological data suggested a possible transdifferentiation of brown into white adipocytes, but this hypothesis has now been challenged by the characterization of distinct precursor cells for brown and white adipocytes in mice. Increased nutrient storage in fully differentiated muscle fibres and adipocytes, resulting in cell enlargement (hypertrophy), is thought to be the main mechanism, whereby muscle and fat masses increase in growing cattle. Competition or prioritization between adipose and muscle cells for the uptake and metabolism of nutrients is suggested, besides the successive waves of growth of muscle v. adipose tissue, by the inhibited or delayed adipose tissue growth in bovine genotypes exhibiting strong muscular development. This competition or prioritization occurs through cellular signalling pathways and the secretion of proteins by adipose tissue (adipokines) and muscle (myokines), putatively regulating their hypertrophy in a reciprocal manner. Further work on the mechanisms underlying cross-talk between brown or white adipocytes and muscle fibres will help to achieve better understanding as a prerequisite to improving the control of body growth and composition in cattle.

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“…Their interest was demonstrated by the fact that their adipose conversion process exhibits some differences when compared to that of cell lines. These studies were essentially performed with rat preadipose cells but preadipocytes from murine, rabbit, ovine, porcine, bovine and human species were also successfully cultured [27,67,92,107,113,152,174,182,216]. These cells can be isolated from different fat depots, as well as from animals of different ages and/or different physiological state [92,104,139].…”

Section: Discussionmentioning

confidence: 99%

The adipose conversion process: regulation by extracellular and intracellular factors

Boone

Mourot

Gregoire³

et al. 2000

Reprod. Nutr. Dev.

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-White adipose tissue regulates lipid metabolism and acts as a secretory organ. Because of its importance for human health and animal production, many studies have attempted to better understand its development at the cellular and molecular levels by culturing preadipose cells in vitro. This synthesis article describes our current knowledge, acquired by this approach, concerning the regulation of the different steps of the adipocyte differentiation program by extracellular (hormones, cytokines, growth factors, retinoids and fatty acids) and intracellular agents (second messengers and transcription factors). The discrepant effects that have been observed for some of these factors are also discussed. This information is very important in the perspective of a better control of fat deposits in human and breeding species.preadipocyte / differentiation / hormonal agent / second messenger / transcription factor Résumé -Le processus d'adipoconversion : sa régulation par des facteurs extracellulaires et intracellulaires. Le tissu adipeux blanc régule le métabolisme lipidique et agit comme un organe de sécrétion. Étant donné son importance pour la santé humaine et la production animale, de nombreuses études ont tenté de mieux comprendre son développement aux niveaux cellulaire et moléculaire en utilisant des cultures de préadipocytes. Cet article de synthèse décrit notre connaissance actuelle, issue de cette approche, concernant la régulation des différentes étapes du programme de la diffé-renciation adipocytaire par des facteurs extracellulaires (hormones, cytokines, facteurs de croissance, rétinoïdes et acides gras) et intracellulaires (seconds messagers et facteurs de transcription). Les effets divergents observés pour certains de ces facteurs sont également discutés. Ces informations sont très importantes dans la perspective d'un meilleur contrôle des dépôts adipeux chez l'humain et les espèces d'élevage.préadipocyte / différenciation / hormone / second messager / facteur de transcription Reprod. Nutr. Dev. 40 (2000) 325-358 325

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Regulation of differentiation of sheep subcutaneous and abdominal preadipocytes in culture

Cited by 55 publications

References 39 publications

Heifer nutrient intake during early- and mid-gestation programs adult offspring adiposity and mRNA expression of growth-related genes in adipose depots

Heifer nutrient intake during early- and mid-gestation programs adult offspring adiposity and mRNA expression of growth-related genes in adipose depots

Ontogenesis of muscle and adipose tissues and their interactions in ruminants and other species

The adipose conversion process: regulation by extracellular and intracellular factors

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