Sap1 Promotes the Association of the Replication Fork Protection Complex With Chromatin and Is Involved in the Replication Checkpoint in <i>Schizosaccharomyces pombe</i>

Noguchi, Chiaki; Noguchi, Eishi

doi:10.1534/genetics.106.065334

Cited by 34 publications

(43 citation statements)

References 57 publications

(94 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We also examined DNA damage-repair foci formation by monitoring the homologous recombination (HR) factor Rad52. Consistent with previous work (22), ∼55% of sap1-1 cells contained one or more Rad52 foci, whereas 11% of WT cells contained a single Rad52 focus (Fig. S4C).…”

Section: Nuclear Peripheral Association Of Regions Containing Replicasupporting

confidence: 92%

“…S4D). Taken together, our study and others suggest that cells carrying a mutation in Sap1 experience problems with replication progression, ultimately resulting in the accumulation of DNA damage (22).…”

Section: Nuclear Peripheral Association Of Regions Containing Replicasupporting

confidence: 76%

“…Sap1 is an abundant nuclear protein that binds specific DNA elements distributed across the genome (17,18). Multiple functions for Sap1 have been proposed, including a role in replication fork blocking (19)(20)(21), replication checkpoint activation (22), and genome organization (17). To gain further insight, we examined Sap1 subcellular localization.…”

Section: Resultsmentioning

confidence: 99%

“…To explore this possibility, we used a partial lossof-function mutant, sap1-1 (22). At restrictive temperature, sap1-1 showed loss of Sap1 nuclear signal (Fig.…”

Section: Significancementioning

confidence: 99%

See 3 more Smart Citations

Shelterin components mediate genome reorganization in response to replication stress

Mizuguchi

Taneja

Matsuda

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The dynamic nature of genome organization impacts critical nuclear functions including the regulation of gene expression, replication, and DNA damage repair. Despite significant progress, the mechanisms responsible for reorganization of the genome in response to cellular stress, such as aberrant DNA replication, are poorly understood. Here, we show that fission yeast cells carrying a mutation in the DNA-binding protein Sap1 show defects in DNA replication progression and genome stability and display extensive changes in genome organization. Chromosomal regions such as subtelomeres that show defects in replication progression associate with the nuclear envelope in sap1 mutant cells. Moreover, highresolution, genome-wide chromosome conformation capture (Hi-C) analysis revealed prominent contacts between telomeres and chromosomal arm regions containing replication origins proximal to binding sites for Taz1, a component of the Shelterin telomere protection complex. Strikingly, we find that Shelterin components are required for interactions between Taz1-associated chromosomal arm regions and telomeres. These analyses reveal an unexpected role for Shelterin components in genome reorganization in cells experiencing replication stress, with important implications for understanding the mechanisms governing replication and genome stability.T he 3D organization of the eukaryotic genome creates specialized microenvironments that play important roles in various nuclear processes (1). In addition to regulation of gene expression, proper execution of the DNA replication program and DNA damage repair also involve dynamic organization of the genome (2, 3). Indeed, computational analyses indicate spatial segregation of replication origins based on their timing of firing during S-phase (4), suggesting that besides local chromatin structure, the 3D organization of chromosomes is an important contributing factor in the spatiotemporal control of replication (5, 6). Despite important advances, understanding the connections between diverse chromosomal events and genome organization remains an important challenge.The complexities of genome organization have been studied in several model organisms, including the fission yeast Schizosaccharomyces pombe, for which the 3D organization has been explored at high resolution (7,8). This simple eukaryote contains the basic chromosomal elements of more complex systems, including partitioning of the genome into euchromatin and heterochromatin domains. The S. pombe genome conforms to a Rabl organization pattern in which centromere clusters and telomere clusters are anchored at opposing sides of the nuclear periphery (9, 10). In addition, two key elements shape genome architecture: cohesin-dependent locally crumpled 50-to 100-kb repeating elements called "globules" on chromosome arms and the constraints imposed by the compaction of the chromatin fiber by heterochromatin domains such as at pericentromeric regions (8). The relatively small size of the S. pombe genome combined with the conserved genome...

show abstract

Section: Nuclear Peripheral Association Of Regions Containing Replicasupporting

confidence: 92%

Section: Nuclear Peripheral Association Of Regions Containing Replicasupporting

confidence: 76%

Section: Resultsmentioning

confidence: 99%

“…To explore this possibility, we used a partial lossof-function mutant, sap1-1 (22). At restrictive temperature, sap1-1 showed loss of Sap1 nuclear signal (Fig.…”

Section: Significancementioning

confidence: 99%

See 2 more Smart Citations

Shelterin components mediate genome reorganization in response to replication stress

Mizuguchi

Taneja

Matsuda

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…2 A). Other notable proteins included Sap1, which has a role in DNA replication and mating-type switching (31,32), telomere associated proteins (such as Ccq1 and Rap1) implicated in heterochromatin assembly (22,33), and components of the microtubule organizing center known as the spindle pole body (SPB) (Fig. 2 A and Fig.…”

Section: Swi6mentioning

confidence: 99%

Diverse roles of HP1 proteins in heterochromatin assembly and functions in fission yeast

Fischer

Cui

Dhakshnamoorthy

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

133

175

View full text Add to dashboard Cite

A vector system for genomic FLAG epitope‐tagging in Schizosaccharomyces pombe

Noguchi

Garabedian

Malik

et al. 2008

Biotechnology Journal

Self Cite

View full text Add to dashboard Cite

The fission yeast Schizosaccharomyces pombe is a popular model organism to study various cellular processes, although research tools available for S. pombe are relatively inadequate. To facilitate genetic and biochemical investigation in S. pombe, we report here a system of vectors for genomic FLAG epitope-tagging. These vectors enable us to amplify gene-targeting fragments for integration into specific loci of the S. pombe genome. All vectors in this report were designed to express FLAG epitope-tagged proteins from their endogenous genomic loci. Vectors for N-terminal FLAG epitope-tagging allow us to control protein expression levels using the wild-type nmt1 promoter, its weaker derivatives, and the urg1 promoter. These vectors are available with various antibiotic markers including kanMX6, hphMX6, natMX6 and bleMX6, and the his3(+) marker. Vectors for C-terminal FLAG epitope-tagging were designed to express FLAG-fusion proteins under the control of their native promoters at their own genomic loci, allowing us to characterize protein functions under physiological conditions. These vectors are available with kanMX6, hphMX6, nat-MX6 and bleMX6 markers. The series of vectors described in this report should prove useful for protein studies in fission yeast.

show abstract

Sap1 Promotes the Association of the Replication Fork Protection Complex With Chromatin and Is Involved in the Replication Checkpoint in Schizosaccharomyces pombe

Cited by 34 publications

References 57 publications

Shelterin components mediate genome reorganization in response to replication stress

Shelterin components mediate genome reorganization in response to replication stress

Diverse roles of HP1 proteins in heterochromatin assembly and functions in fission yeast

A vector system for genomic FLAG epitope‐tagging in Schizosaccharomyces pombe

Contact Info

Product

Resources

About