STAMP2 is required for human adipose-derived stem cell differentiation and adipocyte-facilitated prostate cancer growth in vivo

Abstract

Six Transmembrane Protein of Prostate 2 (STAMP2) has been implicated in both prostate cancer (PCa) and metabolic disease. STAMP2 has unique anti-inflammatory and pro-metabolic properties in mouse adipose tissue, but there is limited information on its role in human metabolic tissues. Using human adipose-derived stem cells (ASCs), we report that STAMP2 expression is dramatically upregulated during adipogenesis. shRNA-mediated STAMP2 knockdown in ASCs significantly suppresses adipogenesis and interferes with optimal expression of adipogenic genes and adipocyte metabolic function. Furthermore, ASC-derived adipocyte-mediated stimulation of prostate tumor growth in nude mice is significantly reduced upon STAMP2 knockdown in ASC adipocytes. These results suggest that STAMP2 is crucial for normal ASC conversion into adipocytes and their metabolic function, as well as their ability to facilitate PCa growth in vivo.

RETRACTED ARTICLE: Long non-coding RNA HIF1A-AS2 facilitates adipose-derived stem cells (ASCs) osteogenic differentiation through miR-665/IL6 axis via PI3K/Akt signaling pathway

“…Recently, adipose-derived stem cells (ASCs) have gained extensive attention on their application in tissue engineering [ 33 ]. Scientists argued ASCs’ own multipotential in differentiation [ 3 ]; apart from adipose cells, they can also play a role in angiogenesis and soft tissue regeneration [ 34 , 35 ], and as a prospective alternative autologous cell-based therapy to bone marrow stem cells [ 36 ], ASC transplant has been successfully applied in bone regeneration [ 37 ]. Evidence suggested that ASCs were analogous to BMSCs in many characteristics: morphology, transcriptome profiles, immunophenotype, and multilineage differentiation function [ 38 – 40 ].…”

Cancer Stem Cells: Powerful Targets to Improve Current Anticancer Therapeutics

“…Conditioned culture media obtained from obese-altered ASC were capable of generating MCF7 breast cancer cell metastasis through the upregulation of ABCB1 and SERPINE1 genes [136]. In prostate cancer, ASC stimulates adipocyte-induced prostate tumor growth through the activation of STAMP2 expression [137]. A recent study demonstrated that coculturing ASC pretreated with paclitaxel inhibited breast cancer proliferation and survival in vitro and in vivo , indicating the potential of this strategy to avoid cancer relapse [138].…”

STEAP4: its emerging role in metabolism and homeostasis of cellular iron and copper

“…At the same time, elevated levels of STEAP4 in prostate cancer cells might also help meet the increased need for iron in rapidly multiply cells, signifying its role in cell growth and maintenance (Korkmaz et al 2005). Thus, while STEAP4 may have a beneficial role in protection from inflammatory stress in a host of chronic metabolic and inflammatory diseases (Grunewald et al 2012; Gomes et al 2012; Wu et al 2015; Jin et al 2015; Tamura & Chiba 2009; Lindstad et al 2010, 2016), its mis-expression may also promote cancer cell proliferation and cancer progression, adding to the complexity in understanding the role of STEAP4 in health and disease.…”