The CENP-A NAC/CAD kinetochore complex controls chromosome congression and spindle bipolarity

McClelland, Sarah E.; Borusu, Satyarebala; Amaro, Ana C.; Winter, Jennifer R.; Belwal, Mukta; McAinsh, Andrew D.; Meraldi, Patrick

doi:10.1038/sj.emboj.7601927

Cited by 76 publications

(145 citation statements)

References 39 publications

(85 reference statements)

Supporting

Mentioning

126

Contrasting

Unclassified

Order By: Relevance

“…3c), as expected from previous studies [29][30][31] . In contrast, CENPH colocalization with CENPA was not affected by several siRNAs targeting the CENPN-pA13 isoform selectively ( Fig.…”

Section: Doxo-regulated Ales Play a Role In Cell Cycle Regulationsupporting

confidence: 90%

See 1 more Smart Citation

A recently evolved class of alternative 3′-terminal exons involved in cell cycle regulation by topoisomerase inhibitors

et al. 2014

View full text Add to dashboard Cite

Alternative 3 0 -terminal exons, which use intronic polyadenylation sites, are generally less conserved and expressed at lower levels than the last exon of genes. Here we discover a class of human genes, in which the last exon appeared recently during evolution, and the major gene product uses an alternative 3 0 -terminal exon corresponding to the ancestral last exon of the gene. This novel class of alternative 3 0 -terminal exons are downregulated on a large scale by doxorubicin, a cytostatic drug targeting topoisomerase II, and play a role in cell cycle regulation, including centromere-kinetochore assembly. The RNA-binding protein HuR/ ELAVL1 is a major regulator of this specific set of alternative 3 0 -terminal exons. HuR binding to the alternative 3 0 -terminal exon in the pre-messenger RNA promotes its splicing, and is reduced by topoisomerase inhibitors. These findings provide new insights into the evolution, function and molecular regulation of alternative 3 0 -terminal exons.

show abstract

“…3c), as expected from previous studies [29][30][31] . In contrast, CENPH colocalization with CENPA was not affected by several siRNAs targeting the CENPN-pA13 isoform selectively ( Fig.…”

Section: Doxo-regulated Ales Play a Role In Cell Cycle Regulationsupporting

confidence: 90%

“…This is consistent with the fact that CENPN association with centromeres is stabilized by its C-terminal domain ( Supplementary Fig. 3d, 'GFP-N DC-ter') [30][31][32] .…”

Section: Doxo-regulated Ales Play a Role In Cell Cycle Regulationsupporting

confidence: 88%

A recently evolved class of alternative 3′-terminal exons involved in cell cycle regulation by topoisomerase inhibitors

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Indeed, when we depleted the CCAN proteins CENP-O or CENP-L, we found that those depletions led to an accumulation of transient monopolar spindles in about 30-40% of the cells McClelland et al, 2007;Mchedlishvili et al, in press;Toso et al, 2009). This suggested that kinetochores are not only driving chromosome movements at the local level, but have a more global effect on spindle architecture.…”

Section: Kinetochore Function Has a Global Effect On The Dynamics Of mentioning

confidence: 90%

Keeping kinetochores on track

Meraldi

2012

European Journal of Cell Biology

Self Cite

View full text Add to dashboard Cite

a b s t r a c tThe multiple functions of kinetochores are reflected in their complex composition, with over a hundred different proteins, which self-associate in several functional subcomplexes. Most of these kinetochore proteins were identified over the last 10-12 years using a combination of genetic, cell biological, biochemical, and bioinformatic approaches in various model organisms. The key challenge since then has been to determine the structural architecture of kinetochores, define the functions of its different subcomponents, and understand its regulation, both in response to the rapid changes in microtubule dynamics or to sense erroneous attachments for spindle checkpoint signalling. Here, we present some of the key advances obtained in the last six years on the biology of kinetochores, both through our work and through the work of many other groups studying this exciting structure.

show abstract

“…CENP-N depletion causes a loss of multiple other centromere components (Foltz et al 2006;McClelland et al 2007;Carroll et al 2009), so CENP-N appears to be a key building block of the centromere. Indeed, S. cerevisiae CENP-N Chl4 is required for de novo centromere formation (Mythreye and Bloom 2003).…”

Section: The Centromerementioning

confidence: 99%

“…However, CENP-N Chl4 is not required to sustain previously established centromeres, suggesting it may function redundantly at existing centromeres. In addition, centromeric CENP-N protein levels decrease before mitosis, suggesting that CENP-N may not be required to recruit kinetochore proteins (McClelland et al 2007;Hellwig et al 2011). Although CENP-N binds the CATD, this may not be necessary for CENP-N to localize to centromeres; centromeres generated by LacI-CENP-C and LacI-CENP-T fusions, which are proposed to be negative for CENP-A, are still positive for CENP-N (Gascoigne et al 2011), and CENP-N can be recruited to H3/CENP-A chimeras that lack the CATD in vitro (Guse et al 2011).…”

Section: The Centromerementioning

confidence: 99%

The Centromere: Epigenetic Control of Chromosome Segregation during Mitosis

Westhorpe

Straight

2014

Cold Spring Harb Perspect Biol

140

View full text Add to dashboard Cite

A fundamental challenge for the survival of all organisms is maintaining the integrity of the genome in all cells. Cells must therefore segregate their replicated genome equally during each cell division. Eukaryotic organisms package their genome into a number of physically distinct chromosomes, which replicate during S phase and condense during prophase of mitosis to form paired sister chromatids. During mitosis, cells form a physical connection between each sister chromatid and microtubules of the mitotic spindle, which segregate one copy of each chromatid to each new daughter cell. The centromere is the DNA locus on each chromosome that creates the site of this connection. In this review, we present a brief history of centromere research and discuss our current knowledge of centromere establishment, maintenance, composition, structure, and function in mitosis.

show abstract

The CENP-A NAC/CAD kinetochore complex controls chromosome congression and spindle bipolarity

Cited by 76 publications

References 39 publications

A recently evolved class of alternative 3′-terminal exons involved in cell cycle regulation by topoisomerase inhibitors

A recently evolved class of alternative 3′-terminal exons involved in cell cycle regulation by topoisomerase inhibitors

Keeping kinetochores on track

The Centromere: Epigenetic Control of Chromosome Segregation during Mitosis

Contact Info

Product

Resources

About