Virulence and potential pathogenicity of coccoid<i>Helicobacter pylori</i>induced by antibiotics

She, Fei Fei; Su, Dong Hui; Lin, Jian; Zhou, Lin

doi:10.3748/wjg.v7.i2.254

Cited by 27 publications

(16 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…26 The coccoid form of the bacteria was also shown to contribute to the development of relapses following antimicrobial therapy in H. pylori infection. 27 Furthermore, in our study, we induced the coccoid form of H. pylori by supplementing the culture medium with tetracycline hydrochloride, a method that has been shown to reduce CoA transferase activity and increase α-ketoglutarate oxidoreductase activity of the induced coccoid bacteria. 20 Such changes in enzymatic activities involved in bacterial energy metabolism not only indicate that the bacteria remain viable during the transformation from spiral to coccoid form, but also suggest that target-specific antibiotics effective in inhibiting one form of the bacteria may not be effective in another.…”

Section: Resultsmentioning

confidence: 94%

Antibacterial Activities of Liposomal Linolenic Acids against Antibiotic-Resistant Helicobacter pylori

et al. 2012

View full text Add to dashboard Cite

Helicobacter pylori (H. pylori) infection with its vast prevalence is responsible for various gastric diseases including gastritis, peptic ulcers, and gastric malignancy. While effective, current treatment regimens are challenged by a fast-declining eradication rate due to the increasing emergence of H. pylori strains resistant to existing antibiotics. Therefore, there is an urgent need to develop novel antibacterial strategies against H. pylori. In this study, we developed a liposomal nanoformulation of linolenic acid (LipoLLA) and evaluated its bactericidal activity against resistant strains of H. pylori. Using a laboratory strain of H. pylori, we found that LipoLLA was effective in killing both spiral and coccoid forms of the bacteria via disrupting bacterial membranes. Using a metronidazole-resistant strain of H. pylori and seven clinically isolated strains, we further demonstrated that LipoLLA eradicated all strains of the bacteria regardless of their antibiotic resistance status. Furthermore, under our experimental conditions, the bacteria did not develop drug resistance when cultured with LipoLLA at various sub-bactericidal concentrations, whereas they rapidly acquired resistance to both metronidazole and free linolenic acid (LLA). Our findings suggest that LipoLLA is a promising antibacterial nanotherapeutic to treat antibiotic-resistant H. pylori infection.

show abstract

Section: Resultsmentioning

confidence: 94%

Antibacterial Activities of Liposomal Linolenic Acids against Antibiotic-Resistant Helicobacter pylori

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Al igual que la forma bacilar, la forma cocoide expresa importantes genes de virulencia, como ureA, ureB, hpaA, vacA y cagA, cagE y BabA 35,53,54 . Esta expresión ocurre por un largo tiempo, y probablemente juegue un rol importante en la enfermedad crónica y severa del estómago.…”

Section: Virulencia Y Patogenicidad De La Forma Cocoideunclassified

Rol de las formas cocoides de Helicobacter pylori en la infección y la recrudescencia

Sarem

Corti²

2016

Gastroenterología y Hepatología

View full text Add to dashboard Cite

“…Coccoid H. pylori is characterized by low virulence (including changes in cell adhesion, urease enzyme activity, cellular vacuolating cytotoxin, etc.) and a weak inflammatory response; therefore, it cannot be removed easily . H. pylori that presents in gastric cancer is usually in the coccoid form, and the coccoid H. pylori detection rate in gastric cancer tissue is significantly higher than that of in ulceration .…”

Section: Introductionmentioning

confidence: 99%

Proliferative and apoptotic effects of gastric epithelial cells induced by coccoid Helicobacter pylori

Han

Chen

et al. 2012

J. Basic Microbiol.

View full text Add to dashboard Cite

Helicobacter pylori exhibit morphology convertion in a spiral or coccoid form. This study aims to reveal the impact of coccoid H. pylori on the proliferation and apoptosis of gastric epithelial cells. The cagA and vacA genes of H. pylori were detected by semi-quantitative RT-PCR. Proliferation and apoptosis were analyzed by the CCK-8 colorimetric method and TUNEL assay, respectively. Egr-1 mRNA and PCNA expression affected by the ERK1/2-specific inhibitor were detected by RT-PCR and immunochemistry. At low density of infection (MOI < 125:1), coccoid H. pylori exerted a stronger effect on proliferation and a weaker effect on apoptosis than did spiral form. The ERK1/2-specific inhibitor significantly blocked the increased expression in Egr-1 and PCNA induced by coccoid H. pylori. Expression of vacA and cagA in coccoid H. pylori decreased compared with the spiral form, whereas vacA decreased more than cagA. The difference of proliferation and apoptosis may be related to the unequal decreased expression of vacA and cagA in coccoid H. pylori. Activation of the ERK1/2-Egr-1-PCNA signal transduction pathway may play an important role in coccoid H. pylori-induced cell proliferation. Long latency of the coccoid form of H. pylori in gastric tissue may be associated with gastric cancer caused by H. pylori.

show abstract

Virulence and potential pathogenicity of coccoidHelicobacter pyloriinduced by antibiotics

Abstract: The virulence and the proteins with molecular weight over M(r)74000 in coccoid H.pylori decrease, but no deletion exists in amplification fragments from ureA, ureB, hpaA, vacA and cagA genes, suggesting that coccoid H.pylori may have potential pathogenicity.

Cited by 27 publications

References 31 publications

Antibacterial Activities of Liposomal Linolenic Acids against Antibiotic-Resistant Helicobacter pylori

Antibacterial Activities of Liposomal Linolenic Acids against Antibiotic-Resistant Helicobacter pylori

Rol de las formas cocoides de Helicobacter pylori en la infección y la recrudescencia

Proliferative and apoptotic effects of gastric epithelial cells induced by coccoid Helicobacter pylori

Contact Info

Product

Resources

About