The mechanism of selenium-mediated salt tolerance has not been fully clarified. This study investigated the possible role of selenium (Se) in regulating maize salt tolerance. A pot experiment was conducted to investigate the role of Se (0, 1, 5 and 25 μM Na2SeO3) in photosynthesis, antioxidative capacity and ion homeostasis in maize under salinity. The results showed that Se (1 μM) relieved the salt-induced inhibitory effects on the plant growth and development of 15-day-old maize plants. Se application (1 μM) also increased the net photosynthetic rate and alleviated the damage to chloroplast ultrastructure induced by NaCl. The superoxide dismutase (SOD) and ascorbate peroxidase (APX) activities were increased, and ZmMPK5, ZmMPK7 and ZmCPK11 were markedly up-regulated in the roots of Se-treated plants, likely contributing to the improvement of antioxidant defence systems under salinity. Moreover, 1 μM Se increased K+ in the shoots while decreasing Na+ in the roots, indicating that Se up-regulates ZmNHX1 in the roots, which may be involved in Na+ compartmentalisation under salinity. The findings from this single experiment require repetition together with measurement of reactive oxygen species (ROS), but nevertheless suggest that exogenous Se alleviates salt stress in maize via the improvement of photosynthetic capacity, the activities of antioxidant enzymes and the regulation of Na+ homeostasis.

The objective of this study was to investigate the effect of Selenium (Se) supply (0,3,6,12,24 ) increased MDA content by 28%, decreased net photosynthetic rate and carboxylation efficiency by 34% and 39%, respectively. The Se concentration in the roots, stems, and leaves of the tobacco plants significantly increased with increasing Se application. A linear correlation (R = 0.95, P < 0.01) was observed between Se level and tobacco plant tissue Se concentration. This correlation indicated that the tobacco plant tissues were not saturated within the concentration range tested. The pattern of total Se concentration in the tobacco plant tissues followed the order root > leaf > stem. The Se concentration in the roots was 3.17 and 7.57 times higher than that in the leaves and stems, respectively, after treatment with 24 mg kg −1 Se. In conclusion, the present study suggested that optimal Se dose (6 mg kg −1 ) improved the plant growth mainly by enhancing photosynthesis, stomatal conductance, carboxylation efficiency and Rubisco content in the flue-cured tobacco leaves. However, the inhibition of excess Se on tobacco growth might be due to high accumulation of Se in roots and the damage of photosynthesis in leaves.

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Tobacco-rice rotation is a common farming system in south China, and many tillage practices such as straw mulching, dolomite dust, and quicklime application have been adopted to improve crop production. These agricultural management practices alter soil physical and chemical properties and affect microbial life environment and community composition. In this research, six tillage practices including no tobacco and rice straw mulching (CK), tobacco and rice straw mulching (TrSr), rice straw returning fire (TrSc), tobacco and rice straw mulching with dolomite dust (TSD), rice straw returning fire and quicklime (TSQ), and rice straw returning fire, quicklime and reduced fertilizer (TSQf) were conducted to detect changes in soil bacterial diversity and composition using Illumina sequencing. The results showed that the total number of operational taxonomic units (OTUs) from the six treatments was 2030, and the number of mutual OTUs among all samples was 550. The TrSc treatment had the highest diversity and richness, while TSQf had the lowest. Soil physio-chemical properties and microbial diversity can influence each other. Proteobacteria and Actinobacteria had the greatest proportion in all treatments. The abundance of Nitrospirae was the highest in the TrSc treatment. The TSQf treatment had the highest abundance of Firmicutes. The abundance of Nitrospira in the TrSc treatment was 2.29-fold over CK. Streptomyces affiliated with Firmicutes improved by 37.33% in TSQf compared to TSQ. TSQf treatment was considered to be the most important factor in determining the relative abundance at the genus level.

Excess N-fertilizer application and inappropriate fertilization methods have led to low N use efficiency (NUE) and high N leaching. A field experiment was performed in a typical lime concretion black soil area to compare N application methods: split surface broadcasting (SSB) and one-time root-zone fertilization (RZF) on grain yield, NUE, the fate of 15N urea and soil N loss during the 2015 and 2016 maize growing seasons. Each application method was tested at N rates of 135 and 180 kg N ha−1, and a control (CK) with no N fertilizer. The RZF treatment remarkably increased grain yield by 7.0% compared with SSB treatment under 180 kg N ha−1, and significantly increased N derived from fertilizer by 28.5%. The residual 15N in the 0–80 cm soil layer was 40.6–47.6% after harvest, 61.8–70.9% of which was retained in 0–20 cm. The RZF remarkably increased the 15N recovery in maize by 28.7%, while significantly decreased the potential N losses by 30.2% compared with SSB in both seasons. In conclusion, one-time RZF of urea is recommended for obtaining high yields, increasing NUE, and minimizing N losses in maize, which deserves more attention for developing and applying in the future.

ABSTRACT.Carotenoids are important components of plant antioxidant systems, which protect photosystems from photooxidative destruction during ultraviolet-B (UV-B) exposure. The influence of carotenoids on total antioxidant capacity (TAC) of plants has rarely been studied. In this study, tobacco (Nicotiana tabacum L., 'K326') seedlings exposed to UV-B radiation were used in order to evaluate the effects of ambient levels of UV-B radiation on carotenoid accumulation. The aim was to investigate whether carotenoids could enhance TAC as a means of UV protection. Our results showed that leaf carotenoid content in the low UV-B exposure (+9.75 mW/cm 2 ) plants was approximately 8% higher than that observed in control plants at 2-8 days of exposure. At high UV-B exposure (+20.76 mW/cm 2 ), the carotenoid content increased rapidly after 1 day's exposure (10.41% higher than the control), followed by a return to the content as in control plants. Furthermore, carotenoid content positively correlated with TAC (P = 0.024). These results suggest that carotenoids have antioxidant properties and play an important role in the antioxidant system. UV-B exposure increased the carotenoid synthesis capability of plants. The plants could deplete the carotenoids to scavenge excess ROS at high UV-B radiation levels, which protects the tobacco plant from oxidative damage caused by UV-B stress.

Improvement in fertilization methods, including the optimal matching of nutrient supply and root nutrient absorption by applying nitrogen (N) in the root zone of crop, is necessary to improve N use efficiency (NUE), maintain high stable yield cultivation of maize, and contribute toward future environmental protection. The current practice of split surface broadcasting (SSB) of N is labor-intensive and the surface broadcasting causes a large amount of N to leach into the environment, yet it does not substantially increase maize yield. Root zone fertilization (RZF) has been identified as an efficient way to solve such problems. However, information on the appropriate amount of N fertilizer under RZF for summer maize remains limited. Therefore, in this study, a two-year consecutive field experiment was conducted during 2015–2016 in Anhui province, China, to investigate the effect of N rate and application method on grain yield, nutrient uptake, and NUE of summer maize. The method chosen is not only important to increase grain yield but also critical for reducing N rate and potential loss in the maize cropping system. The experiment comprised six N rates (90, 135, 180, 225, 270, and 360 kg N hm−2) and two N application methods in both 2015 and 2016. The two N application methods included SSB and one-time RZF. Results showed that grain yield of summer maize increased first and then decreased with the increase of N rate; however, when the N rate increased to 270 kg hm−2, the grain yield increased slowly or even decreased. Compared with SSB, RZF increased grain yield by 4%, and the effect of N on grain yield was mainly related to the number of kernels per ear and 1000-seed weight. One-time RZF increased N apparent recovery efficiency by 18% (7.2 percentage points) compared with SSB and also improved the N agronomic efficiency, N physiological efficiency, and N partial factor productivity. In the comprehensive consideration of yield target, NUE, and soil N balance, the optimal N rate for summer maize in the vertisol soil of Anhui province was 180–225 kg hm−2 for one-time RZF, which reduced N fertilizer by 14% compared with the SSB. Overall, one-time RZF has great potential for green and sustainable agriculture, and thus fertilization machines are worthy of development and application in maize cropping systems.

Selenium (Se) accumulation in plant foods may be providing dietary Se to minimize the health problems related to Se deficiency. In this study, rice plants were cultivated in different naturally seleniferous soils (0.5–1.5 mg Se kg−1). Se concentration in rice plant tissues was analysed, and the distribution and translocation of Se in rice were also studied. The effect of exogenous Se on yield and Se concentration in rice grain was also investigated by spraying Na2SeO3 (15 mg L−1, 15 g ha−1). Results show that Se concentration in root, straw and grain of rice was increased with increased concentrations of Se in seleniferous soils. The root accumulated higher Se than straw and grain under the same naturally seleniferous soil. Spraying Se significantly increased Se concentration in grain, hull, brown rice and polished rice compared with spraying water. Se concentration in the grain fractions was in the following order: Bran > brown rice > whole grain > polished rice > hull. About 13.7% Se in wholegrain was discarded by milling process if about 6.9% of it was polished as bran. Se-enriched rice could be produced in naturally seleniferous soils with Se concentration from 0.5 to 1.0 mg kg−1, and this polished rice would provide enough Se (60–80 μg day−1) to satisfy the human requirement. Therefore, naturally seleniferous soils may be an effective way to produce Se-enriched rice without spraying Se fertilizer, which will be more economically feasible and environmentally friendly for without exogenous Se added to the soils or plants. However, the polished rice and brown rice, produced by spraying Na2SeO3 (15 g ha−1) or grown in soil with total Se upto 1.5 mg kg−1 was not suitable for daily human consumption, unless diluted with Se-deficient rice to meet the standard (≤0.3 mg Se kg−1). This study imparted a better understanding of the utilization of seleniferous soils and Se-enriched rice for human health and food safety.