Hypoxia is defined by low oxygen concentration in organs, tissues, and cells. Maintaining oxygen homeostasis represents the essential cellular metabolic process for the structural integrity of tissues in different pathological conditions, including severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. Considering the role of hypoxia-inducible factor-1 as the regulator of cellular response to hypoxia and its involvement in angiogenesis, erythropoiesis, glucose metabolism, inflammation, we propose hypoxic preconditioning (HPC) as a novel prevention therapeutic approach on healthy contacts of patients with coronavirus disease-2019 (COVID-19). To date, several studies revealed the beneficial effects of HPC in ischemia, kidney failure, and in pulmonary function recovery of patients who underwent lung surgery. HPC increases the expression of factors that promote cell survival and angiogenesis, induces an anti-inflammatory outcome, triggers coordinated hypoxia responses that promote erythropoiesis, and mobilizes the circulating progenitor cells. Furthermore, the mesenchymal stem cells (MSC) exposed to HPC show improvement of their regenerative capacities and increases the effectiveness of stem cell therapy in different pathologies, including COVID-19. In conclusion, HPC should be considered as an approach with beneficial outcomes and without significant side effects when the organism is severely exposed to the same stressor. HPC appears as a trigger to mechanisms that improve and maintain tissue oxygenation and repair, a main goal in different pathologies, including COVID-19 or other respiratory conditions.

Producing parts by 3D printing based on the material extrusion process determines the formation of air gaps within layers even at full infill density, while external pores can appear between adjacent layers making prints permeable. For the 3D-printed medical devices, this open porosity leads to the infiltration of disinfectant solutions and body fluids, which might pose safety issues. In this context, this research purpose is threefold. It investigates which 3D printing parameter settings are able to block or reduce permeation, and it experimentally analyzes if the disinfectants and the medical decontamination procedure degrade the mechanical properties of 3D-printed parts. Then, it studies acetone surface treatment as a solution to avoid disinfectants infiltration. The absorption tests results indicate the necessity of applying post-processing operations for the reusable 3D-printed medical devices as no manufacturing settings can ensure enough protection against fluid intake. However, some parameter settings were proven to enhance the sealing, in this sense the layer thickness being the most important factor. The experimental outcomes also show a decrease in the mechanical performance of 3D-printed ABS (acrylonitrile butadiene styrene) instruments treated by acetone cold vapors and then medical decontaminated (disinfected, cleaned, and sterilized by hydrogen peroxide gas plasma sterilization) in comparison to the control prints. These results should be acknowledged when designing and 3D printing medical instruments.

This paper investigates the impact of several factors related to manufacturing, design, and post-processing on the dimensional accuracy of holes built in the additively manufactured parts obtained by material extrusion process (MEX). Directly fabricated holes in the 3D prints are commonly used for joining with other parts by means of mechanical fasteners, thus producing assemblies or larger parts, or have other functional purposes such as guiding the drill in the case of patient-personalized surgical guides. However, despite their spread use and importance, the relationship between the 3D-printed holes’ accuracy and printing settings is not well documented in the literature. Therefore, in this research, test parts were manufactured by varying the number of shells, printing speed, layer thickness, and axis orientation angles for evaluating their effect on the dimensional accuracy of holes of different diameters. In the same context of limited existing information, the influence of material, 3D printer, and slicing software is also investigated for determining the dimensional accuracy of hole-type features across different manufacturing sites, a highly relevant aspect when using MEX to produce spare or end-use parts in a delocalized production paradigm. The results of this study indicated that the layer thickness is the most relevant influence factor for the diameter accuracy, followed by the number of shells around the holes. Considering the tested values, the optimal set of values found as optimizing the accuracy and printing time was 0.2 mm layer thickness, two shells, and 50 mm/s printing speed for the straight holes. Data on the prints manufactured on different MEX equipment and slicers indicated no statistically significant difference between the diameters of the holes. The evaluation of 3D-printed polylactic acid test parts mimicking a surgical template device with inclined holes showed that the medical decontamination process had more impact on the holes’ dimensional variability than on their dimensional accuracy.

Additively manufactured wrist–hand orthoses (3DP-WHOs) offer several advantages over traditional splints and casts, but their development based on a patient’s 3D scans currently requires advanced engineering skills, while also recording long manufacturing times as they are commonly built in a vertical position. A proposed alternative involves 3D printing the orthoses as a flat model base and then thermoforming them to fit the patient’s forearm. This manufacturing approach is faster, cost-effective and allows easier integration of flexible sensors as an example. However, it is unknown whether these flat-shaped 3DP-WHOs offer similar mechanical resistance as the 3D-printed hand-shaped orthoses, with a lack of research in this area being revealed by the literature review. To evaluate the mechanical properties of 3DP-WHOs produced using the two approaches, three-point bending tests and flexural fatigue tests were conducted. The results showed that both types of orthoses had similar stiffness up to 50 N, but the vertically built orthoses failed at a maximum load of 120 N, while the thermoformed orthoses could withstand up to 300 N with no damages observed. The integrity of the thermoformed orthoses was maintained after 2000 cycles at 0.5 Hz and ±2.5 mm displacement. It was observed that the minimum force occurring during fatigue tests was approximately −95 N. After 1100–1200 cycles, it reached −110 N and remained constant. The outcomes of this study are expected to enhance the trust that hand therapists, orthopedists, and patients have in using thermoformable 3DP-WHOs.

The increasing radiofrequency (RF) electromagnetic radiation pollution resulting from the development and use of technologies utilizing RF has sparked debate about the possible biological effects of said radiation. Of particular concern is the potential impact on the brain, due to the close proximity of communication devices to the head. The main aim of this study was to examine the effects of long-term exposure to RF on the brains of mice in a real-life scenario simulation compared to a laboratory setting. The animals were exposed continuously for 16 weeks to RF using a household Wi-Fi router and a laboratory device with a frequency of 2.45 GHz, and were compared to a sham-exposed group. Before and after exposure, the mice underwent behavioral tests (open-field test and Y-maze); at the end of the exposure period, the brain was harvested for histopathological analysis and assessment of DNA methylation levels. Long-term exposure of mice to 2.45 GHz RF radiation increased their locomotor activity, yet did not cause significant structural or morphological changes in their brains. Global DNA methylation was lower in exposed mice compared to sham mice. Further research is needed to understand the mechanisms behind these effects and to understand the potential effects of RF radiation on brain function.

Effective wound handling involves understanding the processes caused by several factors, such as the type of wound treated, the healing process, the general health of the patient (e.g., the existence of other diseases), the social environment, and the chemical and physical properties of available dressings. For the treatment of shot or burn wounds, the multilayer matrices that will be made must provide efficient oxygen permeability, but most importantly to simulate the structural and biological characteristics of the extracellular skin matrix (ECM). In order to achieve, by mechano-textile processing technologies, the textile structures that can be used as a base layer in the multilayer matrix, raw materials based on cotton, Lenpur, and bamboo mixed with zinc oxide were selected and analysed in accredited testing laboratories, from the point of view of the physical-mechanical characteristics. The results obtained in the case of the experimental program were analysed from a statistical point of view, the description of the statistical populations being made using a specialized program that allowed the calculation of the distribution parameters: mean, median, and standard deviation. To determine the outliers, for all the characteristics of the experimented variants the Q-Q Plot graphs were used. The information obtained as a result of the statistical analysis will be used to design the textile layers of the multilayer matrix using statistical techniques to create probabilistic prediction models that model the dependent variable Y is the hygroscopicity, depending on the independent variables x1 – linear density, x2 – twist/ply, x3 – breaking force, x4 – elongation at break

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The international situation requires a strengthening of the national security measures, including in the field of CBRN and public health. The upgraded microbiology laboratories from DM/ MND must be operated at full capacity for the operationalization of an integrated Platform for the research and expertise of biological war and bioterrorism agents. This is necessary for reasons of national security, for CBRN defense and for public health, in the context of biological agents of 3 and 4 risk groups’ epidemics. The existing upgraded objectives should be operationalized in order to meet the established scope: scientific research and expertise of biological agents and biological weapons, a laboratory for in vitro analysis and a bio-base for in vivo analysis. The highly secure lab allows working with any high-risk agents: biological, genetic, chemical, radiological, etc., being provided with a special room for the insertion/removal of equipment and their decontamination. Following an increase in the capability requirements concerning laboratories, we have provided in the design concept new technical parameters of the platform and the integration of new compartments with related activities of toxicology, pathology, neuro-psycho-pharmacology, bio-pharmacy, micropharmaceutical, specific testing activities, etc. Creating the integrated platform and its operationalization is necessary in order to meet the requirement of the national security strategy as a collective CBRN defense/protection facility and military-medical scientific research for CBRN medical protection.