There is increasing scientific interest to understand the environmental fate of fluorotelomer alcohols (FTOHs) and fluorotelomer-based products which may break down to FTOHs. Both are expected to enter aqueous waste streams, which would be processed in a wastewater treatment plant and therein subject to microbial biodegradation. We investigated the biodegradation of 3-14C, 1H,1H,2H,2H-perfluorodecanol [CF3(CF2)6(14)CF2CH2CH2OH, 14C-8-2 FTOH] in mixed bacterial culture and activated sludge. 14CO2 and 14C-organic volatiles in the headspace of the sealed bottles and bottles with continuous air flow were analyzed up to 4 months. After sample extraction with acetonitrile, 14C-labeled biotransformation products (metabolites) were quantified by LC/ARC (on-line liquid chromatography/ accurate radioisotope counting) and identified by quadrupole time-of-flight (Q-TOF) mass spectrometry and GC/MSD (mass selective detector). Three metabolites not yet reported in the literature have been identified as CF3(CF2)6(14)CHOHCH3 (7-2 sFTOH), CF3(CF2)6(14)CH=CHCOOH (7-3 unsaturated acid or 7-3 u acid), and CF3(CF2)6(14)CH=CHCONH2 (7-3 u amide) along with five previously reported metabolites [CF3(CF2)6(14)CF2CH2CHO (8-2 FTAL), CF3(CF2)6 (14)CF2CH2COOH (8-2 acid), CF3(CF2)6(14)CF=CHCOOH (8-2 u acid), CF3(CF2)6(14)CH2CH2COOH (7-3 acid), and CF3(CF2)6(14)COOH (PFOA)]. No CF3(CF2)6(14)CF2COOH (14C-PFNA) was observed, indicating that alpha-oxidation does not take place. It was found that strong adsorption to the activated sludge and subsequent transformation, even under continuous air flow, greatly reduced partitioning of 8-2 FTOH or any transformation products to air. CF3(CF2)4COOH (PFHA; perfluorohexanoic acid) was observed and increased in mixed bacterial culture over 28 days and accounted for about 1% of the initial 14C-8-2 FTOH concentration from day 28 to day 90. 14CO2 accounted for 1% of initial 14C in activated sludge with continuous air flow at day 1 and increased over time. In closed bottles, 14CO2 in the headspace of activated sludge medium increased to 12% of the available 14C over 135 days with periodic addition of ethanol, as compared to 3% when no additional ethanol was added. These results show that replenishment of organic carbon enhanced microbial mineralization of multiple--CF2--groups in the fluorocarbon chain of 14C-8-2 FTOH. At day 90 the net increase of fluoride ion in the mixed bacterial culture was 93 microg L(-1), equivalent to 12% of total mineralization (destruction) of the 14C-8-2 FTOH. These results demonstrate that perfluorinated carbon bonds of 14C-8-2 FTOH are defluorinated and mineralized by microorganisms under conditions which may occur in a wastewater treatment plant, forming shorter fluorinated carbon metabolites.
This study investigated the biodegradation potential of 3-(14)C,1H,1H,2H,2H-perfluorodecanol [CF3(CF2)6(14)CF2CH2CH2OH, 14C-labeled 8-2 telomer B alcohol or 14C-labeled 8-2 TBA] by diluted activated sludge from a domestic wastewater treatment plant under aerobic conditions. After sample extraction with acetonitrile, biotransformation products were separated and quantified by LC/ARC (on-line liquid chromatography/accurate radioisotope counting) with a limit of quantification about 0.5% of the 14C counts applied to the test systems. Identification of biotransformation products was performed by quadrupole time-of-flight mass spectrometry. Three transformation products have been identified: CF3(CF2)6(14)CF2CH2COOH (8-2 saturated acid); CF3(CF2)6(14)CF=CHCOOH (8-2 unsaturated acid); and CF3(CF2)6(14)COOH (perfluorooctanoic acid, PFOA), representing 27, 6.0, and 2.1% of the initial 14C mass (14C counts applied) after 28 days, respectively. A transformation product, not yet reported in the literature, has also been observed and tentatively identified as CF3(CF2)6(14)CH2CH2COOH (2H,2H,3H,3H-perfluorodecanoic acid); it accounted for 2.3% of the mass balance after 28 days. The 2H,2H,3H,3H-perfluorodecanoic acid is likely a substrate for beta-oxidation, which represents one of the possible pathways for 8-2 telomer B alcohol degradation. The 8-2 saturated acid and 8-2 unsaturated acid cannot be directly used as substrates for beta-oxidation due to the proton deficiency in their beta-carbon (C3 carbon) and their further catabolism may be catalyzed by some other still unknown mechanisms. The 2H,2H,3H,3H-perfluorodecanoic acid may originate either from the major transformation product CF3(CF2)6(14)CF2CH2COOH or from other unidentified transformation products via multiple steps. Approximately 57% of the starting material remained unchanged after 28 days, likely due to its strong adsorption to the PTFE (poly(tetrafluoroethylene)) septa of the test vessels. No CF3(CF2)6(14)CF2COOH (perfluorononanoic acid) was observed, indicating that alpha-oxidation of CF3(CF2)6(14)CF2CH2COOH did not occur under the study conditions. Several 14C-labeled transformation products that have not yet been identified (each less than 1% of the mass balance) were also observed and together accounted for 7% of the total 14C mass balance after 28 days. It is not clear whether these unidentified transformation products were resulting from further metabolism of 8-2 saturated acid or 8-2 unsaturated acid. The results suggest that perfluorinated acid metabolites such as perfluorooctanoic acid account for only a very small portion of the transformation products observed. Also, the observed volatility and bioavailability of 14C-labeled 8-2 TBA for microbial degradation was markedly decreased as a result of the presence of a strongly adsorbing matrix such as PTFE in the experimental systems. It is apparent that the biological fate of 8-2 telomer B alcohol is determined by multiple degradation pathways, with neither beta-oxidation nor any other enzyme-catalyzed rea...
Experiments were carried out to determine the breakthrough of bacteria through a saturated aquifer sand at three flow velocities and three cell concentrations. Bacteria were either suspended in deionized water or 0.01 mol L -• NaCI solution. Bacterial transport was found to increase with flow velocity and cell concentration but was significantly retarded in the presence of 0.01 mol L -• NaC1. A mathematical model based on the advection-dispersion equation was formulated to describe bacterial transport and retention in porous media. The transport equations for bacteria were solved using the finite difference Crank-Nicolson scheme combined with Newton-Raphson iterations. The best fit of the numerical model to the experimental data was obtained using the downhill simplex optimization technique to minimize the sum of the squares of deviations between model predictions and experimental data by varying three parameters. This numerical model was found to describe the experimental data very well under all the experimental conditions tested. An alternative model (also based on the advection-dispersion equation) was tested against all the experimental data sets, but it did not represent the experimental data as well as the model proposed in this paper. face materials [e.g., Bitton et al., 1974; Wollum and Cassel, 1978; Smith et al., 1985; Parke et al., !986; Tan et al., 1991]. Many environmental factors such as ionic strength and flow velocity of the soil solution and properties of the porous materials have been identified to affect microbial transport in porous media in qualitative terms [e.g., Goldshrnid et al., 1973; Bitton et al., !974; Smith et al., !978; Wollum and Cassel, 1978; Gerba and Bitton, !984; McDowell-Boyer et al., 1986; Fontes et al., 1991; Gannon et al., 1991b; Gammack et al., 1992]. Complex mathematical models have also been developed to describe bacterial transport in porous media [e.g., Corapcioglu and Haridas, 1984, !985; Taylor and Jaffe, 1990]. Despite the experimental and modeling 1Now at Centre for Environmental Mechanics, CSIRO, Canben'a, Australia. efforts, few experimental studies have been designed to test those theoretical and conceptual approaches and to describe the aforementioned environmental factors quantitatively in relation to the microbial transport models developed. Nevertheless, it is generally accepted that bacterial transport can be described by the advection-dispersion equation with modifications to account for growth, decay, attachment, and detachment [e.g., Corapcioglu and Haridas, 1984, 1985; Taylor and Jaffe, 1990; Harvey and Garabedian, 1991; Hornberger et al., 1992; Tan et al., 1992]. Attachment refers to processes such as adsorption and straining that can cause retention of bacteria in a porous medium, and detachment refers to the subsequent dislodgment of bacteria from the surfaces. The attachment and detachment of bacteria are the most important and complex processes affecting bacterial transport and are arguably the least understood. Broadbased attempts have been made t...
A study was conducted to relate the properties of Enterobacter, Pseudomonas, Bacillus, Achromobacter, Flavobacterium, and Arthrobacter strains to their transport with water moving through soil. The bacteria differed markedly in their extent of transport; their hydrophobicity, as measured by adherence to n-octane and by hydrophobic-interaction chromatography; and their net surface electrostatic charge, as determined by electrostatic interaction chromatography and by measurements of the zeta potential. Transport of the 19 strains through Kendaia loam or their retention by this soil was not correlated with hydrophobicities or net surface charges of the cells or the presence of capsules. Among 10 strains tested, the presence of flagella was also not correlated with transport. Retention was statistically related to cell size, with bacteria shorter than 1.0 ,lm usually showing higher percentages of cells being transported through the soil. We suggest that more than one characteristic of bacterial cells determines whether the organisms are transported through soil with moving water.
Determinations were made of the influence of NaCl concentration, cell density, and flow velocity on the transport of Pseudomonas sp. strain KL2 through columns of aquifer sand under saturated conditions. A pulse-type boundary condition was used. The experiments were conducted by using 0.3-m-long Plexiglas columns with an internal diameter of 0.05 m. When a 1-h pulse of a 0.01 M NaCl solution containing 108 cells per ml was added at a flow rate of 10-4 m S-l, the bacterial density in the effluent never exceeded 2.2% of the density of cells added, and only 1.5% of the bacteria passed through the aquifer material. In contrast, when the bacteria were applied in distilled water, the relative cell density in the effluent approached 100%, and 60% of the bacteria were transported through the aquifer solids. Under these conditions, the breakthrough of Pseudomonas sp. strain KL2 was slower than chloride. When the flow rate was 2.0 x 10-4 m S-l, the cell density in the effluent reached 7.3% of that added in 0.01 M NaCl solution, but only 3.9% of the bacteria were transported through the aquifer particles. On the other hand, the density in the effluent approached 100% of that added in deionized water, and 77% of the added bacteria were recovered. When the density of added cells was 109 cells per ml at a flow rate of l0-' m s-1, the densities in the effluent reached 70 and 100% of those added in salt solution and deionized water, respectively, and 44 and 57% of the bacteria were transported through the aquifer solids. Replacement of the NaCl solution with deionized water caused some of the retained cells to be carried through the column. We suggest that the movement of bacteria added to sandy aquifers for bioremediation of contaminated sites may be promoted by modifying the chemical composition of the carrying solution.
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