Microbial production of 2,3-butanediol for industrial applications

Song, Chul-Hwa; Park, Jong Myoung; Chung, Sang Chul; Lee, Sang Yup; Song, Hyohak

doi:10.1007/s10295-019-02231-0

Cited by 117 publications

(79 citation statements)

References 106 publications

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“…In the following, we will demonstrate the applicability and the performance benefits of these extensions by realistic example calculations of relevant E. coli strain designs for production of 2,3-butanediol (2,3-BDO). 2,3-BDO is a bulk chemical whose biobased production received much attention in the field of metabolic engineering [48,49]. The spectrum of its industrial application covers a broad range from cosmetics and nutrition to bioplastic [50].…”

Section: Resultsmentioning

confidence: 99%

An extended and generalized framework for the calculation of metabolic intervention strategies based on minimal cut sets

2020

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The concept of minimal cut sets (MCS) provides a flexible framework for analyzing properties of metabolic networks and for computing metabolic intervention strategies. In particular, it has been used to support the targeted design of microbial strains for bio-based production processes. Herein we present a number of major extensions that generalize the existing MCS approach and broaden its scope for applications in metabolic engineering. We first introduce a modified approach to integrate gene-protein-reaction associations (GPR) in the metabolic network structure for the computation of gene-based intervention strategies. In particular, we present a set of novel compression rules for GPR associations, which effectively speedup the computation of gene-based MCS by a factor of up to one order of magnitude. These rules are not specific for MCS and as well applicable to other computational strain design methods. Second, we enhance the MCS framework by allowing the definition of multiple target (undesired) and multiple protected (desired) regions. This enables precise tailoring of the metabolic solution space of the designed strain with unlimited flexibility. Together with further generalizations such as individual cost factors for each intervention, direct combinations of reaction/gene deletions and additions as well as the possibility to search for substrate co-feeding strategies, the scope of the MCS framework could be broadly extended. We demonstrate the applicability and performance benefits of the described developments by computing (gene-based) Escherichia coli strain designs for the bio-based production of 2,3-butanediol, a chemical, that has recently received much attention in the field of metabolic engineering. With our extended framework, we could identify promising strain designs that were formerly unpredictable, including those based on substrate co-feeding.

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Section: Resultsmentioning

confidence: 99%

An extended and generalized framework for the calculation of metabolic intervention strategies based on minimal cut sets

2020

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“…To select a strain for efficient BDO production from whey, we first assessed strains that can utilize lactose and produce BDO. K. pneumonia, E. cloacae, B. licheniformis, and K. oxytoca can produce BDO from glucose [16]. E. coli BL21-pETRABC carrying the BDO pathway gene cluster from E. cloacae can also efficiently bio-transform glucose into BDO [26].…”

Section: Selection Of K Oxytoca Pdl-0 For Bdo Production From Lactosementioning

confidence: 99%

“…One common method for BDO synthesis is performed under harsh conditions (160–220 °C, 50 bar) with a C 4 hydrocarbon fraction of cracked gases as the substrate [ 9 , 10 ]. However, due to shortage of fossil fuels and increasing global environmental concerns, green production of BDO through microbial fermentation is desirable [ 11 – 16 ]. Renewable resources such as rice waste biomass, sugarcane bagasse hydrolysate, and kenaf core biomass have been used in fermentative production of BDO [ 17 – 19 ].…”

Section: Introductionmentioning

confidence: 99%

Efficient 2,3-butanediol production from whey powder using metabolically engineered Klebsiella oxytoca

et al. 2020

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Background: Whey is a major pollutant generated by the dairy industry. To decrease environmental pollution caused by the industrial release of whey, new prospects for its utilization need to be urgently explored. Here, we investigated the possibility of using whey powder to produce 2,3-butanediol (BDO), an important platform chemical. Results: Klebsiella oxytoca strain PDL-0 was selected because of its ability to efficiently produce BDO from lactose, the major fermentable sugar in whey. After deleting genes pox, pta, frdA, ldhD, and pflB responding for the production of by-products acetate, succinate, lactate, and formate, a recombinant strain K. oxytoca PDL-K5 was constructed. Fedbatch fermentation using K. oxytoca PDL-K5 produced 74.9 g/L BDO with a productivity of 2.27 g/L/h and a yield of 0.43 g/g from lactose. In addition, when whey powder was used as the substrate, 65.5 g/L BDO was produced within 24 h with a productivity of 2.73 g/L/h and a yield of 0.44 g/g. Conclusion: This study demonstrated the efficiency of K. oxytoca PDL-0 for BDO production from whey. Due to its non-pathogenicity and efficient lactose utilization, K. oxytoca PDL-0 might also be used in the production of other important chemicals using whey as the substrate.

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“…To select a strain for e cient BDO production from whey, we rst assessed strains that can utilize lactose and produce BDO. K. pneumonia, E. cloacae, B. licheniformis, and K. oxytoca can produce BDO from glucose [16]. E. coli BL21-pETRABC carrying the BDO pathway gene cluster from E. cloacae can also e ciently bio-transform glucose into BDO [26].…”

Section: Selection Of K Oxytoca Pdl-0 For Bdo Production From Lactosementioning

confidence: 99%

Efficient 2,3-butanediol production from whey powder using metabolically engineered Klebsiella oxytoca

Meng

Cao

Zhang

et al. 2020

Preprint

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Background: Whey is a major pollutant generated by the dairy industry. To decrease environmental pollution caused by the industrial release of whey, new prospects for its utilization need to be urgently explored. Here, we investigated the possibility of using whey powder to produce 2,3-butanediol (BDO), an important platform chemical. Results: Klebsiella oxytoca strain PDL-0 was selected because of its ability to efficiently produce BDO from lactose, the major fermentable sugar in whey. After deleting genes pox , pta , frdA , ldhD , and pflB responding for the production of by-products acetate, succinate, lactate, and formate, a recombinant strain K. oxytoca PDL-K5 was constructed. Fed-batch fermentation using K. oxytoca PDL-K5 produced 74.9 g/L BDO with a productivity of 2.27 g/L/h and a yield of 0.43 g/g from lactose. In addition, when whey powder was used as the substrate, 65.5 g/L BDO was produced within 24 h with a productivity of 2.73 g/L/h and a yield of 0.44 g/g. Conclusion: This study demonstrated the efficiency of K. oxytoca PDL-0 for BDO production from whey. Due to its non-pathogenicity and efficient lactose utilization, K. oxytoca PDL-0 might also be used in the production of other important chemicals using whey as the substrate.

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Microbial production of 2,3-butanediol for industrial applications

Cited by 117 publications

References 106 publications

An extended and generalized framework for the calculation of metabolic intervention strategies based on minimal cut sets

An extended and generalized framework for the calculation of metabolic intervention strategies based on minimal cut sets

Efficient 2,3-butanediol production from whey powder using metabolically engineered Klebsiella oxytoca

Efficient 2,3-butanediol production from whey powder using metabolically engineered Klebsiella oxytoca

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