Skeletal Muscle Stem Cells from Animals I. Basic Cell Biology

Dodson, Michael V.; Hausman, Gary; Guan, Leluo; Du, Min; Rasmussen, Theodore P.; Poulos, Sylvia P.; Mir, P. S.; Bergen, Werner G.; Fernyhough, Melinda E.; McFarland, Douglas C.; Rhoads, R; Soret, B.; Reecy, James M.; Velleman, Sandra G.; Jiang, Zhihua

doi:10.7150/ijbs.6.465

Cited by 52 publications

(47 citation statements)

References 103 publications

(88 reference statements)

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“…Interest in using cattle for research studies has increased during the past few years for other reasons. Sufficient (isolatable) adipose tissue is present in all adipose depots of cattle for cell isolation and comparative evaluation (Dodson et al, 2010b) and a broadening database of research tools is rapidly being developed for bovine adipose research models. Furthermore, previous studies have indicated that the physiology of adipocytes derived from cattle may be different from other well-understood adipocyte models (such as 3T3-L1) (Fernyhough et al, 2007;Taniguchi et al, 2008a,b;Dodson et al, 2010a,c;Poulos et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Adenovirus-mediated interference of FABP4 regulates mRNA expression of ADIPOQ, LEP and LEPR in bovine adipocytes

Wei¹,

Liu²,

Wang³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Fatty acid binding protein 4 (FABP4) is an important adipocyte gene, with roles in fatty acid transport and fat deposition in animals as well as human metabolic syndrome. However, little is known about the functional regulation of FABP4 at the cellular level in bovine. We designed and selected an effective shRNA (small hairpin RNA) against bovine FABP4, constructed a corresponding adenovirus (AD-FABP4), and then detected its influence on mRNA expression of four differentiation-related genes (PPARᵧ, CEBPA, CEBPB, and SREBF1) and three lipid metabolism-related genes (ADIPOQ, LEP and LEPR) of adipocytes. The FABP4 mRNA content, derived from bovine adipocytes, decreased by 41% (P < 0.01) after 24 h and 66% (P < 0.01) 495©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 12 (1): 494-505 (2013) shRNA interference of FABP4 in bovine adipocytes after 72 h of AD-FABP4 infection. However, lower mRNA content of FABP4 did not significantly alter levels of differentiation-related gene expression at 24 h following AD-FABP4 treatment of bovinederived preadipocytes (P = 0.54, 0.78, 0.89, and 0.94, respectively). Meanwhile, knocking down (partially silencing) FABP4 significantly decreased ADIPOQ (P < 0.05) and LEP (P < 0.01) gene expression after 24 h of AD-FABP4 treatment, decreased ADIPOQ (P < 0.01) and LEP (P < 0.01) gene expression, but increased LEPR mRNA expression (P < 0.01) after a 72-h treatment of bovine preadipocytes. We conclude that FABP4 plays a role in fat deposition and metabolic syndrome by regulating lipid metabolism-related genes (such as ADIPOQ, LEP and LEPR), without affecting the ability of preadipocytes to differentiate into adipocytes.

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Section: Introductionmentioning

confidence: 99%

Adenovirus-mediated interference of FABP4 regulates mRNA expression of ADIPOQ, LEP and LEPR in bovine adipocytes

Wei¹,

Liu²,

Wang³

et al. 2013

Genet. Mol. Res.

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“…[14][15][16] With the capacity to differentiate into cells of both mesenchymal and nonmesenchymal lineage, the potential application in regenerative medicine has been widely recognized.…”

Section: Mscsmentioning

confidence: 99%

Mesenchymal stromal cells for treatment of the acute respiratory distress syndrome: The beginning of the story

Morrison

McAuley

Krasnodembskaya

2015

Journal of the Intensive Care Society

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In spite of decades of research, the acute respiratory distress syndrome (ARDS) continues to have an unacceptably high mortality and morbidity. Mesenchymal stromal cells (MSCs) present a promising candidate for the treatment of this condition and have demonstrated benefit in preclinical models. MSCs, which are a topic of growing interest in many inflammatory disorders, have already progressed to early phase clinical trials in ARDS. While a number of their mechanisms of effect have been elucidated, a better understanding of the complex actions of these cells may pave the way for MSC modifications, which might enable more effective translation into clinical practice.

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“…Adipose depots are composed of numerous types of cells, including mature adipocytes, preadipocytes, adipofibroblasts, vascular cells, and (perhaps) as-yet-unidentified cells (2,3,(6)(7)(8)(9)(10)(11)(12). Mesenchymal stem cells may become committed to an adipocyte phenotype prior to expressing a differentiated phenotype (2,3,6,13), and cells that are committed to forming lipid-assimilating cells (14)(15)(16) express a variety of sequence-specific intracellular, membrane, and expressed protein markers (2,3,6,9,15,16). Such markers include the peroxisome proliferator-activated receptor (PPAR) family, the most studied of which is PPARg (17).…”

Section: Introductionmentioning

confidence: 99%

Preadipocyte and Adipose Tissue Differentiation in Meat Animals: Influence of Species and Anatomical Location

Hausman¹,

Basu

Wei

et al. 2014

Annu. Rev. Anim. Biosci.

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Early in porcine adipose tissue development, the stromal-vascular (SV) elements control and dictate the extent of adipogenesis in a depot-dependent manner. The vasculature and collagen matrix differentiate before overt adipocyte differentiation. In the fetal pig, subcutaneous (SQ) layer development is predictive of adipocyte development, as the outer, middle, and inner layers of dorsal SQ adipose tissue develop and maintain layered morphology throughout postnatal growth of SQ adipose tissue. Bovine and ovine fetuses contain brown adipose tissue but SQ white adipose tissue is poorly developed structurally. Fetal adipose tissue differentiation is associated with the precocious expression of several genes encoding secreted factors and key transcription factors like peroxisome proliferator activated receptor (PPAR)γ and CCAAT/-enhancer-binding protein. Identification of adipocyte-associated genes differentially expressed by age, depot, and species in vivo and in vitro has been achieved using single-gene analysis, microarrays, suppressive subtraction hybridization, and next-generation sequencing applications. Gene polymorphisms in PPARγ, cathepsins, and uncoupling protein 3 have been associated with back fat accumulation. Genome scans have mapped several quantitative trait loci (QTL) predictive of adipose tissue-deposition phenotypes in cattle and pigs.

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Skeletal Muscle Stem Cells from Animals I. Basic Cell Biology

Cited by 52 publications

References 103 publications

Adenovirus-mediated interference of FABP4 regulates mRNA expression of ADIPOQ, LEP and LEPR in bovine adipocytes

Adenovirus-mediated interference of FABP4 regulates mRNA expression of ADIPOQ, LEP and LEPR in bovine adipocytes

Mesenchymal stromal cells for treatment of the acute respiratory distress syndrome: The beginning of the story

Preadipocyte and Adipose Tissue Differentiation in Meat Animals: Influence of Species and Anatomical Location

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